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Determine the number of genome-containing particles in an AAV prep using qPCR, SYBR Green Technology, and Addgene’s AAV Titration protocol.

Get started with Gibson Assembly Cloning!

Standard protocol for performing agarose gel electrophoresis, including tips to improve resolution and separation of bands.

Protocol to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector.

This protocol describes how to generate a monoclonal cell line from a polyclonal pool of stable cells.

Follow this protocol to produce adeno-associated virus (AAV) in HEK293 cells.