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pAAV-TRE3G-CytoTape-FLAG

Plasmid

#252514

Purpose

Timestamp monomer based on CytoTape for encoding time along protein tape recorder for mouse brain in vivo applications

Depositor

Changyang Linghu

Article

Zheng et al Nature. 2026 Jan 26. doi: 10.1038/s41586

Insert

CytoTape-FLAG

Use

AAV

Expression

Mammalian

Promoter

TRE3G

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.mCherry

Plasmid

#248073

Purpose

iATPSnFR2.A95A.A119L variant with non-responsive mCherry

Depositor

Tim Brown

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.mCherry

Use

AAV

Mutation

A95A.A119L

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.tdTomato

Plasmid

#248074

Purpose

iATPSnFR2.A95A.A119L variant with non-responsive tdTomato

Depositor

Tim Brown

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.tdTomato

Use

AAV

Mutation

A95A.A119L

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.(cyto).iGlucoSnFR2.mIRFP670nano3

Plasmid

#244093

Purpose

Cytosolic expression of green glucose sensor with non-responsive mIRFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Use

AAV

Tags

mIRFP670nano3

Available Since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.(mem).iGlucoSnFR2.mIRFP670nano3

Plasmid

#244098

Purpose

Plasma membrane targeted expression of green glucose sensor with non-responsive mIRFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Use

AAV

Tags

IgG kappa leader and mIRFP670nano3

Available Since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-RiboL1-LifeCamp

Plasmid

#251728

Purpose

Expression of soma-targeted calcium lifetime sensor LifeCamp under hSyn promoter in pAAV backbone

Depositor

Bernardo Sabatini

Article

Lodder et al bioRxiv 2025.12.23.696288

Insert

RiboL1-LifeCamp

Use

AAV

Expression

Mammalian

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV Syn-V5-TurboID-CAAX_v1

Plasmid

#250175

Purpose

Membrane-TurboID variant for neuron expression

Depositor

Christina Kim

Insert

TurboID

Use

AAV

Tags

CAAX and V5

Expression

Mammalian

Promoter

hSyn

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-V5-TurboID-NES

Plasmid

#250171

Purpose

Cytosolic TurboID variant for neuron expression

Depositor

Christina Kim

Insert

TurboID

Use

AAV

Tags

NES and V5

Expression

Mammalian

Promoter

hSyn

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV Syn-V5-TurboID-CAAX_v2

Plasmid

#250176

Purpose

Membrane-TurboID variant for neuron expression

Depositor

Christina Kim

Insert

TurboID

Use

AAV

Tags

CAAX and V5

Expression

Mammalian

Promoter

hSyn

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iGlucoSnFR2.mRuby3

Plasmid

#244071

Purpose

Cytosolic expression of green glucose sensor with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mRuby3

Use

AAV

Tags

mRuby3

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-DIO-optoFGFR1-HA

Plasmid

#250235

Purpose

Cre-dependent AAV expressing light-activatable FGFR1 (optoFGFR1-HA) for temporally precise control of neuronal FGFR1 signaling

Depositor

Won Do Heo

Article

Shin et al Exp Mol Med. 2025 Aug;57(8):1818-1836. d

Insert

FGFR1 (FGFR1 Human)

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

hSyn

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-THP-Synaptophysin-pHluorin

Plasmid

#242903

Purpose

Fluorescent reporter for imaging of synaptic vesicle recycling in Dopaminergic Neurons

Depositor

Timothy Ryan

Article

Pulido et al Proc Natl Acad Sci U S A. 2025 Dec 16;12

Insert

synaptophysin plus pHluorin

Use

AAV

Tags

mouse synaptophysin

Expression

Mammalian

Promoter

rat Tyrosine hydroxylase

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iATPSnFR2.A95K.mCherry

Plasmid

#248071

Purpose

iATPSnFR2.A95K variant with non-responsive mCherry

Depositor

Tim Brown

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.mCherry

Use

AAV

Mutation

A95K

Available Since

Jan. 8, 2026

Availability

Academic Institutions and Nonprofits only

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AAV_CMV-AcrIIA5_cpGR2(GGS5)-N77

Plasmid

#246048

Purpose

Expression of AcrIIA5-cpGR2 hybrid in mammalian cells; insertion before N77; enables chemogenetic control of SauCas9; contains ITRs for AAV packaging with Rep2

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA5_cpGR2-N77

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mCherry-LATeNT

Plasmid

#240989

Purpose

Expresses mCherry-LATeNT in mammalian cells; for AAV transduction of neurons

Depositor

Alice Ting

Article

Roh et al bioRxiv 2025.01.27.635161; doi: 10.1101/

Insert

mCherry-V5-LATeNT

Use

AAV

Tags

V5 (internal)

Expression

Mammalian

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry-TeNT

Plasmid

#240990

Purpose

Expresses mCherry-TeNT in mammalian cells; for AAV transduction of neurons

Depositor

Alice Ting

Article

Roh et al bioRxiv 2025.01.27.635161; doi: 10.1101/

Insert

mCherry-V5-TeNT

Use

AAV

Tags

V5 (internal)

Expression

Mammalian

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVS1-P-6XPyltRNA-dCas9K1151TAG-IRESPylRS

Plasmid

#248695

Purpose

Expression dead CRISPR/Cas9 with unnatural amino acid incorporated at K1151 residue

Depositor

Shuibing Chen

Article

Zhu et al Proc Natl Acad Sci U S A. 2025 Oct 7;122

Insert

dead CRISPR/Cas9 K1151TAG, MbPylRS

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-hCA4-miR122

Plasmid

#244908

Purpose

AAV transgene plasmid encoding human CA4 with miR-122 target site

Depositor

Viviana Gradinaru

Article

Lin et al Cell Rep. 2025 Oct 31;44(11):116419. doi

Insert

human CA-IV

Use

AAV

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAG AAV CMV EGFP-HA

Plasmid

#242963

Purpose

Edit AAV plasmid expressing EGFP-HA, control for plasmid 242962

Depositor

Meghan Rego, Addgene Research Program

Insert

EGFP

Use

AAV

Tags

HA

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAG AAV CMV HA-EGFP

Plasmid

#242961

Purpose

AAV plasmid expressing HA-EGFP, control for plasmid 242960

Depositor

Meghan Rego, Addgene Research Program

Insert

EGFP

Use

AAV

Tags

HA

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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