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pDY0863 PASTEv4 pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

Plasmid

#179106

Purpose

pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

Use

CRISPR

Expression

Mammalian

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-jGCaMP8m-WPRE

Plasmid

#162375

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

Depositor

GENIE Project

Publication

Zhang et al Janelia Research Campus, Online Resource

Has service

AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8m

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

A25G F286Y

Promoter

Synapsin

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

34 Articles

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N8his-GFPenhancer-GGGGS4-LaG16

Plasmid

#140442

Purpose

We designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.

Depositor

Motoyuki Hattori

Publication

Zhang et al Sci Rep. 2020 Apr 10;10(1):6239. doi: 10

Insert

GFPenhancer-GGGGS4-LaG16

Tags

8 histidine tag

Expression

Bacterial

Promoter

T7 promotor

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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CMV-Mito4x-GCaMP6f

Plasmid

#127870

Purpose

Fluorescent reporter for mitochondrial matrix Ca2+

Depositor

Timothy Ryan

Publication

Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

Insert

4 repeats of COX8 signal peptide plus GCaMP6f

Expression

Mammalian

Promoter

CMV

Available since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pTSin EGFP

Plasmid

#127695

Purpose

Plasmid contains the entire replication competent Sindbis genome with the structural genome components replaced by EGFP in an MCS locus. See Resource Information section for annotated plasmid sequence

Depositor

Justin English, Bryan Roth

Publication

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

EGFP

Use

Synthetic Biology

Available since

July 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

Plasmid

#107941

Purpose

Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions

Depositor

Keith Joung, Benjamin Kleinstiver

Publication

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

human codon optimized enAsCas12a (E174R/S542R/K548R)

Tags

3x HA and NLS (nucleoplasmin)

Expression

Mammalian

Mutation

E174R, S542R and K548R

Promoter

CAG

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pmGFP-P2A-K0-P2A-RFP

Plasmid

#105686

Purpose

Double fluorescent stalling reporter K0

Depositor

Ramanujan Hegde

Publication

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Inserts

P2A-3xFLAG-VHPbeta
K0
P2A-mCherry

Expression

Mammalian

Promoter

CMV (from vector)

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pcDNA3.1-TFEB-WT-MYC (#647)

Plasmid

#99955

Purpose

mammalian expression

Depositor

James Brugarolas

Publication

Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

Insert

TFEB (TFEB Human)

Tags

6xHis and Myc

Expression

Mammalian

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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lentiGuide-Hygro-dTomato

Plasmid

#99376

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.

Depositor

Kristen Brennand

Publication

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Inserts

S. pyogenes sgRNA cassette
Hygro-P2A-dTomato

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a and hU6

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pmScarlet_alphaTubulin_C1

Plasmid

#85045

Purpose

In vivo visualization of microtubules (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Alpha-Tubulin (TUBA1B Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA 3.1(Hygro) myc-hPolQ-Flag

Plasmid

#73132

Purpose

To express in mammalian cells human PolQ

Depositor

Agnel Sfeir

Publication

Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

Insert

Polymerase theta (POLQ Human)

Tags

Flag and myc

Expression

Mammalian

Promoter

cmv promoter

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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PP7_mCherry

Plasmid

#61763

Purpose

Expresses PP7 coat protein fused to mCherry

Depositor

Daniel Larson

Publication

Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

Insert

PP7 coat protein fused to mCherry

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

Ubc promoter

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3/Flag-WTAP

Plasmid

#53741

Purpose

For expression of WTAP (Wilms tumor 1 associated protein) in human cells

Depositor

Chuan He

Publication

Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

Insert

Wilms tumor 1 associated protein (WTAP Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

T7

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pCAG-Autobow

Plasmid

#45182

Depositor

Joshua Sanes

Publication

Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

Insert

pCAG-Autobow

Expression

Mammalian

Promoter

CAG

Available since

Nov. 19, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pZac2.1 gfaABC1D-tdTomato

Plasmid

#44332

Depositor

Baljit Khakh

Publication

Shigetomi et al J Gen Physiol. 2013 Apr 15.

Has service

AAV5

Insert

tdTomato

Use

AAV; Adeno-associated virus ()

Expression

Mammalian

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

36 Articles

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tetO-ALN

Plasmid

#43918

Depositor

John Gearhart

Publication

Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

Insert

ASCL1-2A-LMX1B-2A-NURR1 (ASCL1, LMX1B, NR4A2 Human)

Use

Lentiviral; Doxycycline inducible

Tags

6xHis and V5

Expression

Mammalian

Mutation

Gylcine-serine-glycine (GSG) linker followed by v…

Promoter

TRE promoter, Tet-ON

Available since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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AP2u2-mCherry

Plasmid

#27672

Depositor

Christien Merrifield

Publication

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Ap2m1 (Ap2m1 Rat)

Tags

mCherry

Expression

Mammalian

Available since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pAAV-EF1a-FLEX-GTB

Plasmid

#26197

Depositor

Edward Callaway

Publication

Haubensak et al Nature. 2010 Nov 11. 468(7321):270-6.

Insert

Enhanced green fluorescent protein, TVA, rabies B19 glycoprotein

Use

AAV and Cre/Lox; Adeno-associated virus

Mutation

TVA, rabies glycoprotein are mammalian codon opti…

Available since

Nov. 11, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pLM-mCerulean-cMyc

Plasmid

#23244

Depositor

Michel Sadelain

Publication

Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

Insert

mCerulean-2A-MYC (MYC Human, synthetic fluorescent protein)

Use

Lentiviral

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pcDNA5/FRT/TO GFP

Plasmid

#19444

Depositor

Harm Kampinga

Publication

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

GFP

Expression

Mammalian

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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