165,567 results

1. pHAGE-CDKN2A

   Plasmid

   #116726

   Purpose

   Lentiviral expression of CDKN2A

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   CDKN2A (CDKN2A Human)
   

   Use

   Lentiviral

   Mutation

   WT

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Villinpromoter-blue-FlpOERT2

   Plasmid

   #67278

   Purpose

   4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context

   Depositor

   Jannik Elverløv-Jakobsen

   Article

   Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do

   Insert

   Villin-mTQ2-P2A-FlpO-ERT2
   

   Tags

   mTQ2, linked to FlpO through an P2A ribosomal ski…

   Expression

   Mammalian

   Promoter

   Villin

   Available Since

   July 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-Sport6-CD63-pHluorin

   Plasmid

   #130901

   Purpose

   Expression of CD63-pHluorin for visualization of multivesicular body-plasma membrane fusion.

   Depositor

   DM Pegtel

   Article

   Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

   Insert

   CD63-pHluorin (CD63 Human, Aequorea victoria)
   

   Tags

   pHluorin

   Expression

   Mammalian

   Mutation

   pHluorin inserted between Gln-36 and Leu-37 in ex…

   Promoter

   CMV

   Available Since

   Oct. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. EB1-tdTomato

   Plasmid

   #50825

   Purpose

   expresses EB1-tdTomato fusion in mammalian cells for labeling the growing tips of MTs

   Depositor

   Erik Dent

   Insert

   EB1 (Mapre1 Mouse)
   

   Tags

   tdTomato

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNEU-hMbPylRS-4xU6M15

   Plasmid

   #105830

   Purpose

   Plasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cells

   Depositor

   Irene Coin

   Article

   Serfling et al Nucleic Acids Res. 2017 Nov 21. pii: 464

   Inserts

   Humanized MbPylRS
   tRNAM15
   

   Expression

   Mammalian

   Mutation

   (Y271A, Y349F)MbPylRS

   Promoter

   CMV and U6

   Available Since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSG5-HA-p300

   Plasmid

   #89094

   Purpose

   mammalian expression of full-length human p300 in pSG5

   Depositor

   Elizabeth Wilson

   Article

   Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

   Insert

   p300 (EP300 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-S5E2-dTom-nlsdTom

   Plasmid

   #135630

   Purpose

   AAV vector to drive the expression of dTomato in PV cortical interneurons under the control of the E2 regulatory element

   Depositor

   Jordane Dimidschstein

   Article

   Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

   Has Service

   AAV PHP.eB, AAV1, and AAV9

   Insert

   dTom-nlsdTom
   

   Use

   AAV

   Mutation

   None

   Available Since

   Aug. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGP-pcDNA3.1 Puro-CAG-Voltron2-ST

   Plasmid

   #172910

   Purpose

   Mammalian expression of voltage sensor; soma localization targeting sequence

   Depositor

   GENIE Project

   Article

   Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

   Insert

   Voltron2-ST
   

   Tags

   soma localization targeting sequence

   Expression

   Mammalian

   Mutation

   A122D

   Promoter

   CAG

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZE27GFP

   Plasmid

   #75452

   Purpose

   Plasmid for constitutive expression of gfp

   Depositor

   James Collins

   Article

   Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

   Insert

   gfp
   

   Expression

   Bacterial

   Promoter

   placIq promoter

   Available Since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28 His6-mEGFP-D4H

    Plasmid

    #226373

    Purpose

    Production of recombinant mEGFP-D4H, a fluorescent probe binding cholesterol

    Depositor

    Catherine Tomasetto

    Article

    Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

    Insert

    D4H (pfoA Clostridium perfringens)
    

    Tags

    His6 and monomeric EGFP

    Expression

    Bacterial

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-UCOE-SFFV-Zim3-dCas9-P2A-BFP

    Plasmid

    #188767

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Promoter

    SFFV

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLXSN16E6E7

    Plasmid

    #52394

    Purpose

    The plasmid, pLXSN16E6E7, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV16 E6 and E7 genes.

    Depositor

    Denise Galloway

    Article

    Halbert et al J Virol. 1991 Jan;65(1):473-8.

    Insert

    HPV16 E6E7
    

    Use

    Retroviral

    Available Since

    May 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-Camk2-NES-Twitch-GR

    Plasmid

    #173044

    Purpose

    TnC-based calcium sensor with green-red FRET pair ratiometric readout

    Depositor

    Yiyang Gong

    Article

    Zhang et al Commun Biol. 2021 Jul 29;4(1):924. doi:

    Insert

    NES-Twitch-GR
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CamK2

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLIX403-ccdB-G418

    Plasmid

    #158561

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. PBCAG-eGFP

    Plasmid

    #40973

    Depositor

    Joseph Loturco

    Article

    Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

    Inserts

    PiggyBac transposon 3' terminal repeats
    PiggyBac 5' terminal repeats
    

    Expression

    Mammalian

    Available Since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcs2-ATF4uORF1-2-GFP-IRES-mCherry

    Plasmid

    #226105

    Purpose

    ATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR)

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Insert

    atf4 (ATF4 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains uORF1-2 of ATF4, whose stability can ser…

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CBh-mKate2-IRES-MCS (WT-WT)

    Plasmid

    #105921

    Purpose

    AAV-mediated gene expression from CBh promoter. Contains canonical left and right AAV2 ITRs

    Depositor

    Marcella Patrick

    Has Service

    AAV2

    Inserts

    mKate2
    IRES-Multiple cloning site
    

    Use

    AAV

    Mutation

    codon optimized

    Promoter

    CBh and same CBh promoter as mKate2

    Available Since

    Feb. 15, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pCas

    Plasmid

    #62225

    Purpose

    Constitutive expression of cas9 and inducible expression of lambda RED and sgR

    Depositor

    Sheng Yang

    Article

    Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

    Has Service

    Cloning Grade DNA

    Insert

    cas9
    

    Use

    CRISPR; Lambda red recombinase

    Expression

    Bacterial

    Promoter

    native cas9 promoter

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. PGC-1 alpha promoter 2kb luciferase

    Plasmid

    #8887

    Depositor

    Bruce Spiegelman

    Article

    Handschin et al Proc Natl Acad Sci U S A. 2003 Jun 10. 1

    Insert

    PGC-1 alpha promoter (Ppargc1a Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7-NSP1SA11

    Plasmid

    #89168

    Purpose

    Express Rotavirus SA11 NSP1 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 NSP1
    

    Expression

    Mammalian

    Promoter

    T7 promotor

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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