165,688 results

1. pYC-N

   Plasmid

   #207113

   Purpose

   HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus

   Depositor

   Yifan Cheng

   Article

   Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pWM_12x601_30bpLinker

   Plasmid

   #157789

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with 30 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   12x601_30bp linker
   

   Expression

   Bacterial

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CMV-Mito4x-GCaMP6f

   Plasmid

   #127870

   Purpose

   Fluorescent reporter for mitochondrial matrix Ca2+

   Depositor

   Timothy Ryan

   Article

   Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

   Insert

   4 repeats of COX8 signal peptide plus GCaMP6f
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. qTAG-AAVS1-Ef1a-Blast

   Plasmid

   #227267

   Purpose

   Empty AAVS1 targeting donor for the insertion of Blast and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMSP1E3D1

   Plasmid

   #20066

   Purpose

   Bacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   Denisov et al J Biol Chem. 2007 Mar 9. 282(10):7066-76

   Insert

   MSP1E3D1
   

   Tags

   7-His

   Expression

   Bacterial

   Mutation

   "extended" MSP1D1; contains repeats of …

   Available Since

   Jan. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pX330A_dCas9-1x4

   Plasmid

   #63598

   Purpose

   Expresses dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   humanized S. pyogenes dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBiFC-VC155

   Plasmid

   #22011

   Purpose

   Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

   Depositor

   Chang-Deng Hu

   Article

   Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

   Insert

   VC155
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Changed Alanine 206 to Lysine

   Available Since

   Sept. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MSCV

   Plasmid

   #24828

   Depositor

   Lin He

   Article

   Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Grp1-PH pEGFP-C1

   Plasmid

   #71378

   Purpose

   For analysis of PH domain localization in mammalian cells

   Depositor

   Mark Lemmon

   Article

   Kavran et al J Biol Chem. 1998 Nov 13;273(46):30497-5

   Insert

   Grp1-PH (Cyth3 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Pleckstrin homology (PH) domain; aa 247-399

   Promoter

   CMV

   Available Since

   Nov. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET MBP GFP LIC cloning vector (MBP-msfGFP)

    Plasmid

    #29750

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis, AviTag (biotin), MBP, SiriusGFP, and TEV c…

    Expression

    Bacterial

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pArk313

    Plasmid

    #200257

    Purpose

    Ark313 rep/cap

    Depositor

    Justin Eyquem

    Article

    Nyberg et al Cell. 2023 Jan 19;186(2):446-460.e19. do

    Insert

    Ark313 Rep/Cap
    

    Use

    AAV

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSAM_AraC

    Plasmid

    #91569

    Purpose

    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.

    Depositor

    Harry Mobley

    Article

    Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.

    Insert

    AraC/PBAD
    

    Expression

    Bacterial

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. T5-pAc5.1 EGFP-EcoRV

    Plasmid

    #206481

    Purpose

    Schneider cell imaging

    Depositor

    Anne Ephrussi

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Type

    Empty backbone

    Tags

    EGFP

    Expression

    Insect

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX330S-4

    Plasmid

    #58780

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHR_PGK_antiCD19_synNotch_Gal4VP64

    Plasmid

    #79125

    Purpose

    Lentiviral vector for constitutive expression of the anti-CD19 scFv synNotch Gal4DBDVP64 Receptor (PGK promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Insert

    antiCD19_synNotch_Gal4VP64
    

    Use

    Lentiviral

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJAF205

    Plasmid

    #60407

    Purpose

    Flag-tagged full length GMAP210/Trip11

    Depositor

    Gregory Pazour

    Article

    Follit et al PLoS Genet. 2008 Dec;4(12):e1000315. doi

    Insert

    GMAP210 (Trip11 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pMSP2N2

    Plasmid

    #29520

    Depositor

    Stephen Sligar

    Article

    Grinkova et al Protein Eng Des Sel. 2010 Nov . 23(11):8

    Insert

    MSP2N2
    

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    tandem MSP; His-tag on N-terminus followed by spa…

    Available Since

    May 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pWM_12x601_35bpLinker

    Plasmid

    #157790

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 35 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_35bp linker
    

    Expression

    Bacterial

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TFORF3165

    Plasmid

    #144641

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TRIM28 (TRIM28 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. VinculinTS

    Plasmid

    #26019

    Depositor

    Martin Schwartz

    Article

    Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

    Insert

    Vinculin tension sensor
    

    Expression

    Mammalian

    Available Since

    Sept. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge