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The Z-cad dual fluorescent sensor detects dynamic changes between the epithelial and mesenchymal cellular states.
Toneff MJ, Sreekumar A, Tinnirello A, Hollander PD, Habib S, Li S, Ellis MJ, Xin L, Mani SA, Rosen JM
BMC Biol. 2016 Jun 17;14(1):47. doi: 10.1186/s12915-016-0269-y.
PubMed

Plasmids from Article

ID Plasmid Purpose  
79601FUGW-d2GFP-ZEB1 3'UTRFUGW lentiviral vector with constitutively expressed destabilized GFP (d2GFP) regulated by the human ZEB1 3'UTR. When used in combination with Ecad-RFP, comprises the Z-cad dual sensor.
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79602FUGW-d2GFP-200FUGW lentiviral vector with constiutively expressed destabilized GFP (d2GFP) regulated by 5 miR-200 family binding sites
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79603pHAGE-E-cadherin-RFPpHAGE lentiviral vector with dsRed driven by the human E-cadherin promoter. When used in combination with FUGW-d2GFP-ZEB1 3' UTR, comprises the Z-cad dual sensor. Use 2nd generation packaging system
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81018FUGW-eGFP-ZEB1 3'UTRFUGW lentiviral vector with constitutively expressed eGFP regulated by the human Zeb1 3'UTR. Brighter than destabilized d2GFP sensor reported in manuscript
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81020pINDUCER13 miR-200c-141Lentiviral vector containing doxycycline inducible human miR-200c. Puromycin selectable.
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