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pMApra-Ptac

Plasmid

#218357

Purpose

Empty vector with lacIq-Ptac expression system

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available Since

May 2, 2024

Availability

Academic Institutions and Nonprofits only

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EZ-Tet-pLKO-Hygro

Plasmid

#85972

Purpose

Tet/Dox inducible shRNA lentivirus with hygromycin selection

Depositor

Cindy Miranti

Article

Frank et al BMC Biotechnol. 2017 Feb 28;17(1):24. do

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral and RNAi

Promoter

H1 / TetO

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

Plasmid

#179105

Purpose

pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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FUGW

Plasmid

#14883

Purpose

3rd gen lentiviral plasmid with hUbC-driven EGFP; can be used for cDNA expresion

Depositor

David Baltimore

Article

Lois et al Science. 2002 Feb 1. 295(5556):868-72.

Insert

flap-Ub promoter-GFP-WRE

Use

Lentiviral

Expression

Mammalian

Available Since

May 1, 2007

Availability

Academic Institutions and Nonprofits only

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dCAS9-VP64_GFP

Plasmid

#61422

Purpose

Expresses dCAS9-VP64 activator with 2A GFP

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Has Service

Concentrated Lentiviral Prep

Insert

dCAS9(D10A,H840A)-VP64_2A_GFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and H840A in Cas9

Promoter

EF1A

Available Since

Dec. 15, 2014

Availability

Academic Institutions and Nonprofits only

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DT12A

Plasmid

#80416

Purpose

Expresses 12 CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

Depositor

Thomas Cooper

Article

Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

Insert

human DMPK exons 11-15 with 12 CTG repeats (DMPK Human)

Expression

Mammalian

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-RecQL4

Plasmid

#211399

Purpose

mammalian expression of human RecQL4

Depositor

Helmut Pospiech

Insert

RecQL4 (RECQL4 Human)

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-TRIM28

Plasmid

#124960

Purpose

Mammalian expression of Human TRIM28

Depositor

Michelle Barton

Article

Li et al Oncogene. 2017 May 25;36(21):2991-3001.

Insert

hTRIM28 (TRIM28 Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLCNICK

Plasmid

#84653

Purpose

Genome editing for Lactobacillus casei Lc2W

Depositor

Sheng Yang

Article

Song et al Appl Environ Microbiol. 2017 Sep 1. pii:

Inserts

Cas9 nickase
sgRNA
homology arms of LC2W_2179

Use

CRISPR

Mutation

D10A

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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DT40

Plasmid

#80415

Purpose

Expresses 40 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

Depositor

Thomas Cooper

Article

Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

Insert

human DMPK exons 11-15 with 40 interrupted CTG repeats (DMPK Human)

Expression

Mammalian

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-NPM1wt-IRES-mCherry

Plasmid

#131824

Purpose

Lentiviral plasmid expressing NPM1 wildtype (human) in mammalian cells upon transduction

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

NPM1 (NPM1 Human)

Use

Lentiviral

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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tetO.Sox9.Puro

Plasmid

#117269

Purpose

Expresses Sox9 and puromycin resistance gene under control of tetO sequence.

Depositor

Henrik Ahlenius

Article

Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

Insert

Sox9 (Sox9 Mouse)

Use

Lentiviral

Tags

T2A and puromycin resistant cassette (C terminal …

Expression

Mammalian

Promoter

tet-on

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pIMAY

Plasmid

#68939

Purpose

E. coli/staphylococcal temperature-sensitive plasmid for allelic exchange

Depositor

Tim Foster

Article

Monk et al MBio. 2012 Mar 20;3(2). pii: e00277-11.

Type

Empty backbone

Use

Staphylococcal allelic exchange vector

Available Since

Sept. 29, 2015

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-pBAD

Plasmid

#54572

Purpose

Localization: Protein Expression Vector , Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

6xHis, T7, and mTagBFP2

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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TFORF3549

Plasmid

#145025

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCAX APP 695

Plasmid

#30137

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

Amyloid Precursor Protein (APP Human)

Expression

Mammalian

Mutation

wild type splice variant 695

Available Since

Aug. 15, 2011

Availability

Academic Institutions and Nonprofits only

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LM670

Plasmid

#191552

Purpose

LM670_MSG-AGGA-promoter-ccdb-TTCC-GShis

Depositor

David Baker

Article

Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

Insert

ccdb

Tags

HIS-tag

Expression

Bacterial

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pET21a-Streptavidin-Alive

Plasmid

#20860

Purpose

Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.

Depositor

Alice Ting

Article

Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

Insert

Streptavidin-Alive

Tags

His

Expression

Bacterial

Mutation

Wt gene

Available Since

April 21, 2009

Availability

Academic Institutions and Nonprofits only

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pTXB1-Tn5

Plasmid

#60240

Purpose

IPTG-inducible expression of Transposon Tn5 fused to Mxe Intein and Chitin-binding domain.

Depositor

Rickard Sandberg

Article

Picelli et al Genome Res. 2014 Jul 30. pii: gr.177881.

Insert

tnpA from pBam1

Tags

Mxe intein - Chitin-binding domain

Expression

Bacterial

Mutation

E54K, L372P

Promoter

T7 promoter

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-STING_gRNA3

Plasmid

#127640

Purpose

Knock-out of human STING

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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