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RFP-KIF1B beta-FL

Plasmid

#79111

Purpose

Mammalian expression of RFP-KIF1B beta-FL

Depositor

Susanne Schlisio

Article

Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi

Insert

RFP-KIF1B beta full length (KIF1B Human)

Use

Tags

RFP

Expression

Mammalian

Mutation

exon 25 missing (natural splice varient)

Promoter

CMV

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only
pGreen2x35SLUC

Plasmid

#108228

Purpose

Plant expression as translational fusions to FLUC with RLUC normalization

Depositor

Alejandro Ferrando

Article

Solé-Gil et al Methods Mol Biol. 2024;2722:79-87. doi:

Type

Empty backbone

Use

Luciferase

Tags

Firefly Luciferase

Expression

Plant

Mutation

Promoter

2x35S

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-vDIO-mScarlet-IRES-tTA

Plasmid

#118030

Purpose

AAV vector for sparse and bright labeling in a cell-type specific manner

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

Insert

TRE-vDIO-mScarlet-IRES-tTA

Use

AAV

Tags

Expression

Mutation

Promoter

TRE

Available since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLEX307-mFzd5

Plasmid

#102867

Purpose

Expresses mouse Fzd5 in mammalian cells

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Frizzled 5 (Fzd5 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

A to G substitution at nucleotide 699 of Fzd5 ORF…

Promoter

EF1A

Available since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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Kv7.4 (1-695)/pcDNA3.1

Plasmid

#111453

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Use

Tags

8xHis and EGFP

Expression

Mammalian

Mutation

Kv7.4 (1-695)

Promoter

Available since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP C1-Eps8 WT

Plasmid

#74950

Purpose

mammalian expression of Eps8 fused to GFP

Depositor

Giorgio Scita

Article

Hertzog et al PLoS Biol. 2010 Jun 1;8(6):e1000387. doi

Insert

Eps8 (Eps8 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 3, 2016

Availability

Academic Institutions and Nonprofits only
pET28_6xHis-PARP1-CATdeltaHD

Plasmid

#174811

Purpose

Bacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-CATdeltaHD (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Residues 678-787 replaced by eight-residue linker…

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-neo FlucDM EGFP

Plasmid

#90172

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutation

Depositor

Franz-Ulrich Hartl

Article

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Use

Tags

Expression

Mammalian

Mutation

R188Q, R261Q in FLuc

Promoter

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-mAmetrine-CNA35

Plasmid

#61604

Purpose

bacterial expression of N-terminal fusion protein of CNA35 with mAmetrine

Depositor

Maarten Merkx

Article

Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

Insert

mAmetrine and CNA35

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pPM2213

Plasmid

#167553

Purpose

Encodes rfb region of Shigella flexneri serotype 2a, which expresses Shigella O antigen in E. coli K-12

Depositor

Renato Morona

Article

Macpherson et al Mol Microbiol. 1991 Jun;5(6):1491-9. doi

Insert

rfb region of Shigella flexneri serotype 2a

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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GAL-hAR-658-919

Plasmid

#89082

Purpose

mammalian expression of human androgen receptor ligand binding domain 658-919 amino acids linked to GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2007 Aug 31;282(35):25801-1

Insert

hAR-658-919 (AR Human)

Use

Tags

GAL4 DBD

Expression

Mammalian

Mutation

aa 658-919

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-hSNCA

Plasmid

#170445

Purpose

Lentiviral vector expressing human SNCA, under control of EF1alpha promoter.

Depositor

Sang-Hun Lee

Article

Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

Insert

human SNCA (SNCA Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1aL-dsRed-W

Plasmid

#195863

Purpose

lentiviral expression of the red fluorescent protein driven by human EF1aL promoter

Depositor

Darrell Kotton

Type

Empty backbone

Use

Lentiviral

Tags

dsRed

Expression

Mutation

Promoter

EF1aL

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAG1242 NLS-mCherry-FIREtag

Plasmid

#205170

Purpose

Expresses NLS-mCherry-FIREtag in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

NLS-mCherry-FIREtag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pSIN-hSNCA-NE

Plasmid

#102366

Purpose

Lentiviral overexpression of synuclein alpha

Depositor

Shu Leong Ho

Article

Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

Insert

synuclein alpha (SNCA Human)

Use

Lentiviral

Tags

NE

Expression

Mammalian

Mutation

A silent mutation (333bp downstream of ATG) was i…

Promoter

EF-1a

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-LSSmOrange-CNA35

Plasmid

#61605

Purpose

bacterial expression of N-terminal fusion protein of CNA35 with LSSmOrange

Depositor

Maarten Merkx

Article

Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

Insert

LSSmOrange and CNA35

Use

Tags

His tag

Expression

Mutation

Promoter

T7

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 ZoAl N-1F

Plasmid

#203763

Purpose

For expression of N-flag tagged ZoAl R2 protein in mammalian cells

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

R2 retroelement protein

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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dCas9-NS3-NLS/VPR

Plasmid

#112244

Purpose

Encodes the drug-preservable Cas9-NS3-NLS/VPR, which can be used to activate gene expression when combined with an NS3 inhibitor and appropriately designed sgRNA.

Depositor

John Ngo

Article

Tague et al Nat Methods. 2018 Jul;15(7):519-522. doi

Insert

dCas9-NS3-NLS/VPR

Use

Tags

AU1 (N term on NS3) and HA (C term on NS3)

Expression

Mammalian

Mutation

NS3 mutant T54A

Promoter

CMV

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag TYK2

Plasmid

#20660

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

TYK2 (TYK2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
TFORF1999

Plasmid

#143864

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TAL1 (TAL1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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