163,486 results

1. pMRBad-Z-CspGFP

   Plasmid

   #40730

   Depositor

   Brian McNaughton

   Article

   Blakeley et al Mol Biosyst. 2012 Aug;8(8):2036-40. Epub

   Insert

   Leucine Zipper prime - C super positive Green Fluorescent Protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBad

   Available Since

   Nov. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDawn-sfGFP

   Plasmid

   #107741

   Purpose

   Expresses superfolder GFP under the control of a blue-light transcriptional regulator

   Depositor

   Ingmar Riedel-Kruse

   Article

   Jin et al Proc Natl Acad Sci U S A. 2018 Apr 3;115

   Insert

   superfolder GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLambda

   Available Since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET11a-Z-NspGFP

   Plasmid

   #40729

   Depositor

   Brian McNaughton

   Article

   Blakeley et al Mol Biosyst. 2012 Aug;8(8):2036-40. Epub

   Insert

   N super positive GFP - Leucine Zipper
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMIEG3-JunDN

   Plasmid

   #40350

   Depositor

   Alexander Dent

   Article

   Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

   Insert

   Jun dominant negative (JUN Human)
   

   Use

   Retroviral

   Tags

   HA and IRES eGFP

   Expression

   Mammalian

   Mutation

   first 122 amino acids deleted

   Promoter

   Available Since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCTCON2-Aga1p

   Plasmid

   #158141

   Purpose

   Expresses Aga1p in yeast

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   Aga1p
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL10

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. LRRK2_Halo_N_allele

   Plasmid

   #178142

   Purpose

   Donor vector for endogenous tagging of human LRRK2 at the N-terminus with halotag

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Halotag (LRRK2 Human)
   

   Use

   Donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCalN-ddGFP-A

   Plasmid

   #40290

   Depositor

   Robert Campbell

   Article

   Alford et al ACS Synth Biol. 2012 Dec 21;1(12):569-75

   Insert

   Calnexin (CANX Human)
   

   Use

   Tags

   ddGFP-A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. FRB-mCherry-FTH1

   Plasmid

   #100749

   Purpose

   Part of FerriTag expression system. Encodes human Ferritin with an FRB domain and mCherry tag

   Depositor

   Stephen Royle

   Article

   Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

   Insert

   ferritin heavy chain 1 (FTH1 Human)
   

   Use

   Tags

   FRB and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGal4AD-CIB1

   Plasmid

   #28245

   Purpose

   Expresses Gal4 Activation Domain fused to CIB1 for blue-light-induced transcription activation

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   Gal4AD (CIB1, GAL4 Budding Yeast)
   

   Use

   Tags

   CIB1

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available Since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLBH531_MBP-Cas14a1 expression

    Plasmid

    #112500

    Purpose

    Expresses MBP fused to Cas14a1

    Depositor

    Jennifer Doudna

    Article

    Harrington et al Science. 2018 Oct 18. pii: science.aav42

    Insert

    Cas14a1
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNeg-Ma-barnase-TAG3-TAG45

    Plasmid

    #197572

    Purpose

    Negative selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses 2xTAG-codon interrupted barnase gene and M. alvus Pyl-tRNA(6). p15a origin of replication.

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    Barnase - 2xTAG
    M. alvus Pyl-tRNA (6)
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    3TAG and 45TAG

    Promoter

    araC and lpp

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-CMV-MCS-Puro-EGFP

    Plasmid

    #219795

    Purpose

    Mammalian expression plasmids using piggyBac Transposon System

    Depositor

    Keiichi Nakayama

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pV1524

    Plasmid

    #111431

    Purpose

    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-T7pol

    Plasmid

    #59926

    Purpose

    Expression vector for T7 RNA polymerase gene

    Depositor

    Ian Wickersham

    Insert

    T7 RNA polymerase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBAD-Citron1

    Plasmid

    #134300

    Purpose

    Bacterial expression of direct-response citrate sensor

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    Citron1
    

    Use

    Tags

    6-His tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE

    Plasmid

    #20945

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    hSyn

    Available Since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. plRL117

    Plasmid

    #163635

    Purpose

    Optimized M. smegmatis CRISPRi plasmid; L5 integrating.

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet-On

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSJX022

    Plasmid

    #232325

    Purpose

    Genome editing in Caulobacter crescentus

    Depositor

    Wei Zhao

    Article

    Sun et al bioRxiv 2024.12.02.626314

    Inserts

    sgRNA
    SpCas9M
    

    Use

    CRISPR

    Tags

    H-arms and cat

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23119 and Pvan

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSJX016

    Plasmid

    #232324

    Purpose

    Genome editing in Caulobacter crescentus

    Depositor

    Wei Zhao

    Article

    Sun et al bioRxiv 2024.12.02.626314

    Inserts

    sgRNA
    SpCas9M
    

    Use

    CRISPR

    Tags

    H-arms and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23119 and Pvan

    Available Since

    June 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGM1190

    Plasmid

    #69994

    Purpose

    OriT containing mobilizable vector for thiostrepton induced gene expression in Streptomyces

    Depositor

    Günther Muth

    Article

    Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

    Inserts

    aac
    tsr
    rep
    sso
    TipA promoter
    dso
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge