159,258 results

1. pENTR E3 pR7A L3-L2

   Plasmid

   #158734

   Purpose

   Plant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last NI)

   Depositor

   Shin-ichi Arimura

   Article

   Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

   Insert

   TALEN ORF without TAL repeat
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pENTR E1 pF7A L1-L4

   Plasmid

   #158730

   Purpose

   Plant mitoTALEN vector assembly, Entry clone 1 with attR4 R3 (last NI)

   Depositor

   Shin-ichi Arimura

   Article

   Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

   Insert

   TALEN ORF without TAL repeat
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pENTR E1 pF6A L1-L4

   Plasmid

   #158729

   Purpose

   Plant mitoTALEN vector assembly, Entry clone 1 with attR4 R3 (last NG)

   Depositor

   Shin-ichi Arimura

   Article

   Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

   Insert

   TALEN ORF without TAL repeat
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEGFP-Atg14L

   Plasmid

   #21635

   Depositor

   Tamotsu Yoshimori

   Article

   Matsunaga et al Nat Cell Biol. 2009 Apr . 11(4):385-96.

   Insert

   Atg14L (ATG14 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAG-3xFLAG-BioID2-MCS

   Plasmid

   #186811

   Purpose

   CAG promoter-driven expression of BioID2-fused protein (N terminus)

   Depositor

   Masahito Ikawa

   Article

   Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

   Type

   Empty backbone

   Use

   Tags

   3xFLAG-BioID2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pH-nCas9-PBE

   Plasmid

   #98163

   Purpose

   Expresses CRISPR base editor in rice

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

   Insert

   NLS-APOBEC1-XTEN-nCas9-UGI-NLS
   

   Use

   Rice

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Ubi

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 5xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:ECFP

   Plasmid

   #158654

   Purpose

   making transgenics expressing a QUAS driven YFP and NTR 2.0

   Depositor

   Jeff Mumm

   Article

   Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

   Insert

   5xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y;he:eCFP
   

   Use

   Tol2-based transgenic expression in zebrafish/oth…

   Tags

   Expression

   Mutation

   F70A/F108Y

   Promoter

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LZRS-DNMT1

   Plasmid

   #24952

   Depositor

   Paul Khavari

   Article

   Sen et al Nature. 2010 Jan 28. 463(7280):563-7.

   Insert

   DNMT1 (DNMT1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYC1640

   Plasmid

   #158719

   Purpose

   CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using zeocin as a selection marker.

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   Sth1 sgRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pY094

    Plasmid

    #84743

    Purpose

    Expresses huAsCpf1-T2A-GFP and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huAsCpf1
    GFP
    

    Use

    Tags

    3xHA, NLS, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR223-MERTK

    Plasmid

    #23900

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    MERTK (MERTK Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. RET-WT

    Plasmid

    #125252

    Purpose

    Expresses human RET in mammalian cells

    Depositor

    Sissy Jhiang

    Article

    Xing et al Endocrinology. 1996 May;137(5):1512-9. d

    Insert

    RET (RET Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAB061i

    Plasmid

    #79226

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    Ppro1 rpoZ-HA4; PlacIq lacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ppro1; PlacIq

    Available since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC57-sgRNA expression vector

    Plasmid

    #51132

    Purpose

    For in vitro transcription of sgRNA from the T7 promoter.

    Depositor

    Xingxu Huang

    Article

    Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. hCXCR4-mTQ2

    Plasmid

    #110196

    Purpose

    Encodes for human CXCR4 coding sequence tagged with the mTQ2 FP

    Depositor

    Sergi Padilla Parra

    Article

    Starling et al Nat Commun. 2023 Aug 17;14(1):4994. doi:

    Insert

    hCXCR4 (CXCR4 Human)
    

    Use

    Tags

    mTurquoise (mTQ2)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Reporter-2

    Plasmid

    #180219

    Purpose

    Surrogate reporter plasmid for the assay of targeted RNA editing

    Depositor

    Wensheng Wei

    Article

    Qu et al Nat Biotechnol. 2019 Sep;37(9):1059-1069

    Insert

    Surrogate reporter
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAB076i3

    Plasmid

    #79198

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI-SH2ABL1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ-opt (OR1); Ppro1

    Available since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAJE-Ma pylRS nitroY/haloY-F5

    Plasmid

    #225684

    Purpose

    Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control during Ma Pyl tRNA-synthetase validation.

    Depositor

    Ryan Mehl

    Inserts

    M. alvus PylRS 3-NY/HaloY F5
    M. alvus Pyl-tRNA(6)
    

    Use

    Methanomethylophilus alvus

    Tags

    Expression

    Mutation

    CTG125CTT, N166A, V168S, A223C, W239R

    Promoter

    GlnS and Lpp

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJJB302

    Plasmid

    #107694

    Purpose

    OsActin Promoter-LwaCas13a-HSP Terminator

    Depositor

    Daniel Voytas

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    Cas13a
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    OsActin

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-G-Flamp1

    Plasmid

    #188567

    Purpose

    green biosensor for cAMP in mammalian cells

    Depositor

    Jun Chu

    Article

    Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

    Insert

    G-Flamp1
    

    Use

    Tags

    His tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge