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Engineered miniature CRISPR-Cas system for mammalian genome regulation and editing.
Xu X, Chemparathy A, Zeng L, Kempton HR, Shang S, Nakamura M, Qi LS
Mol Cell. 2021 Aug 26. pii: S1097-2765(21)00648-1. doi: 10.1016/j.molcel.2021.08.008.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
176268pSLQ7334: pHR-PGK-SV40_NLS-dCas12f-VPR-c- Myc_NLS-mCherry-WPREExpression of dCas12f-VPR-mCherry in mammalian cells
176269pSLQ9926: pHR-PGK-SV40_NLS-dCasMINI-V4-VPR-c- Myc_NLS-mCherry-WPREExpression of dCasMINI-V4-VPR-mCherry in mammalian cells
176270pSLQ9757: CMV-BPNLS_TadA-linkerA-TadA*(8e)-Linker B-SV40 NLS-dCasMINI-V4-linkerC-c-Myc_NLS-3x FLAG-polyAExpression of TadA-TadA* (8e)-dCasMINI-V4 in mammalian cells
176271pSLQ9824: CMV-SV40_NLS-CasMINI-V3.1--3xFlag- c-Myc_NLS-polyAExpression of CasMINI-V3.1 in mammalian cells.
176272pSLQ9834_sglacZ: pHR-hU6-CasMINI sgRNA_#2 (sgLacZ); EF1a-Puro-T2A-BFP- WPREExpression of non-targeting sgRNA of CasMINI
176273pSLQ9834_sgTet: pHR-hU6-CasMINI sgRNA_#2 (sgTet); EF1a-Puro-T2A-BFP- WPREExpression of CasMINI sgRNA targeting TRE3G promoter in dCasMINI-mediated GFP activation assays.
180280pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPREThe CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

Antibodies from Article