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Plasmid#138840PurposeConstitutive expression of VPR-ZF43 under the CMV promoter.DepositorInsertVPR-ZF43
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
C3AR1-DuET
Plasmid#213191PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertC3AR1 (C3AR1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_BRD4_WT
Plasmid#82323PurposeGateway Donor vector containing BRD4 , part of the Target Accelerator Plasmid Collection.DepositorInsertBRD4 (BRD4 Human)
UseGateway entry vectorTagsExpressionMutationP474LPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MAST1
Plasmid#23855DepositorInsertMAST1 (MAST1 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pPD545 ZF37x6-Compact_EYFP
Plasmid#138859PurposeEYFP reporter vector for the ZF37x6-Compact promoterDepositorInsertZF37x6-Compact
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF37x6-CompactAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nck
Plasmid#186133PurposeFor expression of recombinant Nck in bacteria cells.DepositorInsertNCK1 (NCK1 Human)
UseTagsGSTExpressionBacterialMutationAmino acid residues 1-377, with mutations: C139S,…PromoterLacAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
14UAS zfPSD95:GFP
Plasmid#74314PurposeA fusion of zebrafish PSD95 to GFP under control of 14 UAS repeatsDepositorInsertzebrafish PSD95
UseTagsEGFPExpressionMutationY599H and I793S (please see depositor comment bel…PromoterUASAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPD100 VP16-ZF43
Plasmid#138761PurposeConstitutive expression of VP16-ZF43 under the CMV promoter.DepositorInsertVP16-ZF43
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-OPCML-Fc(DAPA)-AviTag-6xHis
Plasmid#156733PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertOPCML (OPCML Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Human-Dysferlin-v1
Plasmid#60217PurposeHuman dysferlin exon 1 variant (accession #DQ267935) cDNA in pDONR221 plasmid which can be used as an entry vector for Invitrogen’s gateway system.DepositorInsertDysferlin (DYSF Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
GAL-AR-1-503
Plasmid#89093Purposemammalian expression of human androgen receptor N-terminal 1-503 amino acids linked to GAL4 DNA binding domainDepositorInserthAR aa 1-503 (AR Human)
UseTagsGAL4 DBDExpressionMammalianMutationPromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28 His6-mEGFP-D4H
Plasmid#226373PurposeProduction of recombinant mEGFP-D4H, a fluorescent probe binding cholesterolDepositorInsertD4H (pfoA Clostridium perfringens)
UseTagsHis6 and monomeric EGFPExpressionBacterialMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPD536 VP16-ZF150
Plasmid#138769PurposeConstitutive expression of VP16-ZF150 under the CMV promoter.DepositorInsertVP16-ZF150
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD558 ZF172x6-Compact_EYFP
Plasmid#138872PurposeEYFP reporter vector for the ZF172x6-Compact promoterDepositorInsertZF172x6-Compact
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF172x6-CompactAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD538 VP16-ZF172
Plasmid#138772PurposeConstitutive expression of VP16-ZF172 under the CMV promoter.DepositorInsertVP16-ZF172
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF1844
Plasmid#144313PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRB1 (RB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-GPR35
Plasmid#204708Purposeexpresses HA-GPR35 in mammalian cellsDepositorInsertG protein-coupled receptor 35 (GPR35 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pminTolR4-R2
Plasmid#67711Purposemini Tol2 backbone with att4-attR2 cassetteDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
PAS528 (TJF059)
Plasmid#65602PurposepETDuet-1 Containing FadD-HisDepositorInsertFadD
UseTags6x HisExpressionBacterialMutationPromoterT7 lacOAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
YIp TRP1-HIS3
Plasmid#122912PurposeTRP1-HIS3 integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceDec. 3, 2019AvailabilityAcademic Institutions and Nonprofits only