161,641 results

1. pGL4.0-TERT G228A

   Plasmid

   #84926

   Purpose

   luciferase reporter for mutant TERT promoter

   Depositor

   Joseph Costello

   Article

   Bell et al Science. 2015 May 29;348(6238):1036-9. d

   Insert

   TERT promoter (TERT Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   G228A

   Promoter

   TERT

   Available since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRGE32

   Plasmid

   #63159

   Purpose

   Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for transient expression in protoplast

   Depositor

   Yinong Yang

   Article

   Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Rice snoRNA U3 promoter for gRNA/PTG expression a…

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJRH051

   Plasmid

   #171625

   Purpose

   2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-BsmBI-Destination
   mNeon
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a and U6

   Available since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV.CAG.GCaMP6f.WPRE.SV40

   Plasmid

   #100836

   Purpose

   AAV expression of ultrasensitive protein calcium sensor from CAG promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Has Service

   AAV1, AAV2, and AAV9

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

   Promoter

   CAG

   Available since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMF19

   Plasmid

   #113662

   Purpose

   Containing the wbbL (rhamnosyltransferase) gene cloned for mutant complementation

   Depositor

   Miguel Valvano

   Article

   Feldman et al J Biol Chem. 1999 Dec 3;274(49):35129-38

   Insert

   wbbL
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Ptac

   Available since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL4.0-TERT G250A

   Plasmid

   #84925

   Purpose

   luciferase reporter for mutant TERT promoter

   Depositor

   Joseph Costello

   Article

   Bell et al Science. 2015 May 29;348(6238):1036-9. d

   Insert

   TERT promoter (TERT Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   G250A

   Promoter

   TERT

   Available since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

7. pRT029

   Plasmid

   #127969

   Purpose

   double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus

   Depositor

   Martin Kampmann

   Article

   Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

   Insert

   CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Tet-pLKO-neo

   Plasmid

   #21916

   Purpose

   3rd generation lentiviral plasmid for inducible expression of shRNA; neomycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.

   Depositor

   Dmitri Wiederschain

   Article

   Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAPtag5-IgK-NCAN-Full length

   Plasmid

   #219812

   Purpose

   Expresses His-tagged NCAN-Full length to produce recombinant protein

   Depositor

   Cagla Eroglu

   Article

   Irala et al Neuron. 2024 Mar 27:S0896-6273(24)00161-

   Insert

   IgK (Igk Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-mPCSK1

    Plasmid

    #122672

    Purpose

    Expresses mouse PCSK1 in mammalian cells

    Depositor

    Liming Pei

    Article

    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

    Insert

    proprotein convertase subtilisin/kexin type 1 (PCSK1) (Pcsk1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Anti-GFP [N86/38.1R]

    Plasmid

    #114492

    Purpose

    Mammalian Expression Plasmid of anti-GFP. Derived from hybridoma N86/38.1.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXs-3XMyc-EGFP-OMP25

    Plasmid

    #83355

    Purpose

    For tagging mitochondria with Myc epitopes (Control-MITO construct)

    Depositor

    David Sabatini

    Article

    Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

    Insert

    3XMyc-EGFP-OMP25
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-IGF1R-V5/HIS

    Plasmid

    #201979

    Purpose

    expression of human IGF1R receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    IGF1R receptor tyrosine kinase (IGF1R Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-UbC

    Plasmid

    #62806

    Purpose

    To express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.

    Depositor

    Jae Lee, Pantelis Tsoulfas

    Article

    Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

    Type

    Empty backbone

    Use

    AAV; Eucaryotic

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-ABE8eWQ

    Plasmid

    #161815

    Purpose

    Expresses ABE8eWQ in mammalian cells

    Depositor

    Sangsu Bae

    Insert

    bpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAVS1-Puro CAG CLTX-NKG2D-2B4-CD3z CAR

    Plasmid

    #157744

    Purpose

    knock CLTX-NKG2D-2B4-CD3z CAR into AAVS1 safe harbor locus for constitutive expression

    Depositor

    Xiaoping Bao

    Article

    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

    Insert

    chlorotoxin containing chimeric antigen receptor targeting glioblastoma
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pACRISPR

    Plasmid

    #113348

    Purpose

    A sgRNA expression plasmid for genome editing in Pseudomonas aeruginosa

    Depositor

    Quanjiang Ji

    Article

    Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pScPPX2

    Plasmid

    #112877

    Purpose

    Expresses His-tagged yeast exopolyphosphatase for purification from E. coli

    Depositor

    Michael Gray, Ursula Jakob

    Article

    Gray et al Mol Cell. 2014 Mar 6;53(5):689-99. doi:

    Insert

    PPX1 (PPX1 Budding Yeast)
    

    Use

    Tags

    6X His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCI H2B-RFP

    Plasmid

    #92398

    Purpose

    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-mRFP1 reporter.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    IRES H2B RFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AiP12123 - pAAV-AiE0397m-minBglobin-SYFP2-WPRE3-BGHpA (Alias: CN2123)

    Plasmid

    #208150

    Purpose

    Direct-expressing SYFP2 in oligodendrocytes AAV Virus

    Depositor

    The Allen Institute for Brain Science, Boaz Levi

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Beta Globin minimal promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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