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pAAV hSyn FLEx-loxp Kir2.1-2A-GFP

Plasmid

#161574

Purpose

Expression of Kir2.1-2A-GFP in a Cre-dependent fashion

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

Kir2.1-2A-GFP (Kcnj2 Mouse)

Use

AAV

Mutation

NA

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pMApra-Ptac

Plasmid

#218357

Purpose

Empty vector with lacIq-Ptac expression system

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available Since

May 2, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-6XHis-human SUMO 1

Plasmid

#133770

Purpose

express 6Xhis- SUMO 1 protein

Depositor

Martine Roussel

Article

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 1 (SUMO1 Human)

Tags

6XHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR-A-HYG

Plasmid

#29635

Depositor

David_L Andrews, Scott Gold

Article

Garcia-Pedrajas et al Fungal Genet Biol. 2008 Apr . 45(4):379-

Type

Empty backbone

Use

Gateway donor

Available Since

Oct. 5, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO.3G

Plasmid

#14748

Depositor

Christophe Benoist, Diane Mathis

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-MKK6-EE p38alpha

Plasmid

#82719

Purpose

Expresses constitutively active human MKK6 and murine p38 alpha.

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Inserts

MKK6 (MAP2K6 Human)
p38alpha (Mapk14 Mouse)

Expression

Bacterial

Mutation

Ser207 to Glu, Thr211 to Glu

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
SMA-Cherry

Plasmid

#110209

Purpose

Mapping of mesenchymal progenitor cells.

Depositor

David Rowe

Article

Grcevic et al Stem Cells. 2012 Feb;30(2):187-96. doi:

Insert

mCherry

Tags

None

Expression

Mammalian

Promoter

Smooth muscle alpha-actin and first intron

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-3XHA-EGFP-OMP25

Plasmid

#83356

Purpose

For tagging mitochondria with HA epitopes (HA-MITO construct)

Depositor

David Sabatini

Article

Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

Insert

3XHA-EGFP-OMP25

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-H2B-GFP

Plasmid

#116869

Purpose

Nuclear localized GFP

Depositor

Loren Looger

Article

Economo et al Nature. 2018 Nov;563(7729):79-84. doi: 1

Has Service

AAV Retrograde

Insert

GFP

Use

AAV

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pMT3 p38

Plasmid

#12658

Depositor

John Kyriakis

Insert

p38alpha (MAPK14 Human)

Tags

HA

Expression

Mammalian

Available Since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-hygro-mCherry-RAMP4

Plasmid

#118391

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

RAMP4 (SERP1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

EIF-1a promoter

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe puro HA PIK3CA E545K

Plasmid

#12525

Depositor

Jean Zhao

Article

Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

Insert

PIK3CA E545K (PIK3CA Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

E545K. Tumor-derived allele.

Available Since

Aug. 18, 2006

Availability

Academic Institutions and Nonprofits only

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pTW1169

Plasmid

#236583

Purpose

TRV1

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Insert

TRV1

Expression

Plant

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT-HA-rM3D(Gs)

Plasmid

#45549

Purpose

Expresses rM3D (Gs) neuronal activator

Depositor

Bryan Roth

Article

Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

Insert

rM3D (Gs)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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FLAG-FBXL17

Plasmid

#236188

Purpose

Expression of N-terminal tagged FLAG-FBXL17 (H. sapiens, codon-optimized) in human cell lines.

Depositor

Michele Pagano

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

FLAG-FBXL17 (FBXL17 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pmGFP-ADAR1-p150

Plasmid

#117927

Purpose

ADAR1-p150 expression plasmid.

Depositor

Kumiko Ui-Tei

Article

Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

Insert

ADAR1-p150 (ADAR Human)

Tags

myc-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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LM670

Plasmid

#191552

Purpose

LM670_MSG-AGGA-promoter-ccdb-TTCC-GShis

Depositor

David Baker

Article

Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

Insert

ccdb

Tags

HIS-tag

Expression

Bacterial

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLV 4xSTAR-sTomato-NLS-blast

Plasmid

#136259

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

sTomato-NLS

Expression

Mammalian

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a_POU5F1_P2A_Hygro_Barcode

Plasmid

#120474

Purpose

Barcoded lentiviral vector to express POU5F1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

POU5F1 (POU5F1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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Flag_HsIRE1a_pBabePuro

Plasmid

#54337

Purpose

N-terminally FLAG tagged human IRE1a in retroviral pBabe puro vector

Depositor

David Ron

Article

Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

Insert

IRE1a (ERN1 Human)

Use

Retroviral

Tags

FLAG and preprotrypsin signal sequence

Expression

Mammalian

Mutation

The endogenous IRE1a signal sequence (aa1-18) was…

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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