159,333 results

1. pCXLE-mCherry-t2a-SOX2-p2a-KLF4

   Plasmid

   #193296

   Purpose

   episomal mCherry-hSK (expressing mCherry + human SOX2 + KLF4)

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   mCherry-t2a-SOX2-p2a-KLF4 (SOX2, KLF4 Discosoma, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pD649-HAsp-SEMA3E-COMP5AP-AviTag-9xHis

   Plasmid

   #157534

   Purpose

   Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   SEMA3E (SEMA3E Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. M4754 KanMX::HIS3 Disruptor Converter

   Plasmid

   #51684

   Purpose

   Yeast marker swap plasmid for converting KanMX3 to HIS3

   Depositor

   David Stillman

   Article

   Voth et al Yeast. 2003 Aug;20(11):985-93.

   Insert

   HIS3 (HIS3 Budding Yeast)
   

   Use

   Yeast marker swap

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWS5399-CRISPRai-ZeoR

   Plasmid

   #182838

   Purpose

   Saccharomyces cerevisiae inducible CRISPRai vector - ZeoR marker

   Depositor

   Rodrigo Ledesma-Amaro

   Article

   Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_AncBE4max_P2A_GFP

   Plasmid

   #112100

   Purpose

   C:G-to-T:A base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

   Insert

   AncBE4max_P2A_GFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL4.13/AUG-FFLuc-3XFLAG

   Plasmid

   #127333

   Purpose

   Express 3xFLAG tagged firefly luciferase (FFLuc) from AUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   Firefly luciferase
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CV40

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Anti-VGlut2 [N29/29R]

   Plasmid

   #114501

   Purpose

   Mammalian Expression Plasmid of anti-VGlut2 (Rat). Derived from hybridoma N29/29.

   Depositor

   James Trimmer

   Article

   Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

   Insert

   anti-VGlut2 (Rattus norvegicus) recombinant mouse monoclonal antibody (Slc17a6 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   dual CMV

   Available since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCfB2988

   Plasmid

   #63638

   Purpose

   pCfB2988 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

   Depositor

   Jerome Maury, Irina Borodina

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   none

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. FUW-Gata2

   Plasmid

   #193087

   Purpose

   constitutive expression of mouse Gata2 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

   Insert

   Gata2 (Gata2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ubc

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. PEP115E

    Plasmid

    #97401

    Purpose

    binary construct of Golgi-targeted mCherry with sfGFP11 tag for plant expression

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

    Insert

    mCherry-sfGFP11
    

    Use

    Tags

    Golgi target

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCW57-MCS1-P2A-MCS2 (Neo)

    Plasmid

    #89180

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLNHA-C1-Hs4E-BP1

    Plasmid

    #79438

    Purpose

    Expresses lambdaN-HA-tagged human 4E-BP1 in Mammalian cells

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

    Insert

    Hs4E-BP1 (EIF4EBP1 Human)
    

    Use

    Tags

    lambdaN-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. PEP116E

    Plasmid

    #97402

    Purpose

    binary construct of plasma membrane-targeted mCherry with sfGFP11 tag for plant expression

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

    Insert

    mCherry-sfGFP11
    

    Use

    Tags

    Plasma membrane target

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. EFR WT

    Plasmid

    #102413

    Purpose

    fluorescent tagging. Plant expression of EFR WT

    Depositor

    Silke Robatzek

    Insert

    EFR (EFR Mustard Weed)
    

    Use

    Tags

    6xHis and mgfp5

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Nov. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. EF1a_NEUROG3_P2A_Hygro_Barcode

    Plasmid

    #120468

    Purpose

    Barcoded lentiviral vector to express NEUROG3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    NEUROG3 (NEUROG3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXRM15

    Plasmid

    #63150

    Purpose

    Met15 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.

    Depositor

    Marc Gartenberg

    Article

    Chou et al FEMS Yeast Res. 2015 May;15(3):fov010. d

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. p35S::AtSOBIR1-mCherry

    Plasmid

    #86166

    Purpose

    SOBIR1-mCherry expression

    Depositor

    Silke Robatzek

    Article

    Postma et al New Phytol. 2016 Apr;210(2):627-42. doi:

    Insert

    SOBIR1 (SOBIR1 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pαH-S-GSAS/D614G

    Plasmid

    #164566

    Purpose

    SARS-CoV-2 Spike protein with D614G mutation (S-GSAS-D614G variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike (S-GSAS-D614G variant)
    

    Use

    Tags

    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQCXIZ GFP C1

    Plasmid

    #202424

    Purpose

    Encodes GFP alone

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQCXIZ GFP PODXL delta EC

    Plasmid

    #202423

    Purpose

    Expression of GFP-tagged PODXL without extracellular domain

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    PODXL (PODXL Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    deleted extracellular domain

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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