pBabe-Puro-RhoA FLARE.sc Biosensor
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12602||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Gene/Insert nameRhoA Biosensor
Alt name6xHis-RBD-CFP-Linker-Citrine-RhoA WT
SpeciesH. sapiens (human)
Insert Size (bp)2400
Entrez GeneRHOA (a.k.a. ARH12, ARHA, EDFAOB, RHO12, RHOH12)
- Cloning method Restriction Enzyme
- 5′ cloning site blunt (destroyed during cloning)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pBABE-5 (Common Sequencing Primers)
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Protocols for production and use of Hahn lab biosensors and photactivatable proteins
can be found on their web page, at the URL below. If you have
suggestions for the protocols or have any questions, please feel free to contact the Hahn
lab. Good luck with your experiments!
Hahn lab biosensor protocol: http://www.hahnlab.com/tools/index.html
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBabe-Puro-RhoA FLARE.sc Biosensor was a gift from Klaus Hahn (Addgene plasmid # 12602 ; http://n2t.net/addgene:12602 ; RRID:Addgene_12602)
For your References section:Spatiotemporal dynamics of RhoA activity in migrating cells. Pertz O, Hodgson L, Klemke RL, Hahn KM. Nature. 2006 Apr 20. 440(7087):1069-72. 10.1038/nature04665 PubMed 16547516