165,688 results

1. pRL-SV40P

   Plasmid

   #27163

   Purpose

   Renilla SV40 promoter (no enhancer) reporter gene for normalization of luciferase experiments

   Depositor

   Ron Prywes

   Article

   Chen et al Mol Cell Biol. 1999 Jul . 19(7):4695-702

   Insert

   SV40 promoter
   

   Use

   Luciferase

   Tags

   Renilla Luciferase

   Expression

   Mammalian

   Available Since

   Feb. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti PGK Blast DEST (w524-1)

   Plasmid

   #19065

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Blast 

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. lenti-mTagBFP2

   Plasmid

   #216126

   Purpose

   Lentiviral vector for expressing mTagBFP2.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Insert

   EF1a-mTagBFP2-WPRE
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUW-Luc-mCherry-puro

   Plasmid

   #183685

   Purpose

   Lentiviral vector expressing luciferase and mCherry.

   Depositor

   Andrew Kung

   Article

   Kimbrel et al Mol Imaging. 2009 May-Jun;8(3):140-7.

   Insert

   Luc-E2A-mCherry-T2A-PuroR
   

   Use

   Lentiviral

   Promoter

   Human Ubiquitin C (UbC) promoter

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-mCh-Cry2WT

   Plasmid

   #101221

   Purpose

   Blue-light dependent optogenetic protein Cry2

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

   Insert

   Cry2PHR
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Feb. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. lenti sgRNA(MS2)_puro optimized backbone

   Plasmid

   #73797

   Purpose

   optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 and EF1A

   Available Since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. hNav1.8_pcDNA3.1

   Plasmid

   #182847

   Purpose

   Expresses hNav1.8 in mammalian cells

   Depositor

   Paul DeCaen

   Article

   McCollum et al Cell Rep. 2022 Aug 23;40(8):111248. doi:

   Insert

   SCN10A (SCN10A Human)
   

   Expression

   Mammalian

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWPI-N1ICD

   Plasmid

   #185525

   Purpose

   Constitutive overexpression of human NOTCH1 intracellular domain

   Depositor

   Eric Shusta, Sean Palecek

   Article

   Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

   Insert

   NOTCH1 intracellular domain (NOTCH1 Human)
   

   Use

   Lentiviral

   Promoter

   EF-1a

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV-IRES-GFP

   Plasmid

   #20672

   Purpose

   Depositor

   Tannishtha Reya

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   IRES EGFP

   Expression

   Mammalian

   Available Since

   Aug. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-EFS-SaKKHABE8e-bGH-U6-sgRNA-BsmBI

    Plasmid

    #189923

    Purpose

    AAV genome encoding SaKKHABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

    Depositor

    David Liu

    Article

    Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

    Insert

    SaABE8e V106W
    

    Use

    AAV

    Mutation

    nSaCas9 KKH, TadA ABE8e V106W

    Promoter

    EFS

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR5'_ubi:loxP-EGFP-loxP

    Plasmid

    #27322

    Depositor

    Leonard Zon

    Article

    Mosimann et al Development. 2011 Jan . 138(1):169-77.

    Insert

    ubiquitin:loxP-EGFP-loxP
    

    Use

    Multisite gateway 5' entry vector

    Available Since

    April 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. mini-pHelper-1.0

    Plasmid

    #230933

    Purpose

    The mini version (8.4 kb) of Ad helper plasmid to provide E2A, E4orf6, and VA RNA for AAV production

    Depositor

    Qizhao Wang

    Article

    Yang et al Mol Ther Nucleic Acids. 2025 May 14;36(2

    Type

    Empty backbone

    Use

    AAV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMBP-LbCas12a

    Plasmid

    #113431

    Purpose

    Expresses 10xHis-MBP fused to LbCas12a

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Has Service

    Cloning Grade DNA

    Insert

    LbCas12a
    

    Tags

    MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. mTLR4

    Plasmid

    #13085

    Depositor

    Ruslan Medzhitov

    Insert

    TLR4 (Tlr4 Mouse)
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. 8522-M01-663

    Plasmid

    #225660

    Purpose

    Lentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (green configuration)

    Depositor

    Dominic Esposito

    Article

    Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

    Insert

    dsCopGFP
    

    Use

    Lentiviral

    Promoter

    SORE6-mCMVp

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. 35S-eGFP-nosT

    Plasmid

    #80127

    Purpose

    Transient expression vector for eGFP in plants

    Depositor

    Yutaka Kodama

    Article

    Fujii et al Plant Biotech. 2015; 32(1): 81-87.

    Type

    Empty backbone

    Tags

    eGFP

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. myc-BioID2-MCS

    Plasmid

    #74223

    Purpose

    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tag

    Depositor

    Kyle Roux

    Article

    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    Myc and humanized A. aeolicus BioID2 with R40G

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-C1-PLCdelta-PH

    Plasmid

    #21179

    Purpose

    encodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipids

    Depositor

    Tobias Meyer

    Article

    Stauffer et al Curr Biol. 1998 Mar 12. 8(6):343-6.

    Insert

    PLCdelta (PLCD1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PH domain of human PLC delta, amino acids Met1–Il…

    Available Since

    July 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. mito NAD+ biosensor

    Plasmid

    #186791

    Purpose

    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag totarget mitochondria

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    mito NAD+ biosensor
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. AP1muA-Halo-ALFA-polyA-G418 HR

    Plasmid

    #229681

    Purpose

    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells.

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence AP1M1 locus (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    Halo, ALFA

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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