165,775 results

1. pEGFPc1 H-RAS

   Plasmid

   #236449

   Purpose

   transient overexpression of H-RAS in mammalian cells

   Depositor

   Michele Pagano

   Article

   Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

   Insert

   H-RAS (HRAS Human)
   

   Expression

   Mammalian

   Available Since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. AiP2141 - pAAV-AiE2333m-minBG-iCre(R297T)-BGHpA

   Plasmid

   #220731

   Purpose

   AiE2333m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

   Depositor

   Allen Institute for Brain Science, Bosiljka Tasic

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   iCre(R297T)
   

   Use

   AAV

   Promoter

   minBG

   Available Since

   May 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-SARS2-Spike-HiBiT

   Plasmid

   #191212

   Purpose

   Transient expression in mammalian cells of SARS-CoV-2 (A1) spike protein tagged on the C-terminus with HiBiT, the high affinity small fragment of split nanoluciferase assay

   Depositor

   Mohan Babu

   Article

   Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

   Insert

   SARS-CoV-2 spike_WT (S SARS CoV-2)
   

   Tags

   HiBiT (high affinity small nanoluciferase fragmen…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA FRT to BioID-cDIC1-3xFlag

   Plasmid

   #232417

   Purpose

   Expresses human dynein subunit DIC1 in mammalian cells.

   Depositor

   Samara Reck-Peterson

   Article

   Redwine et al Elife. 2017 Jul 18;6:e28257. doi: 10.755

   Insert

   Dynein Intermediate Chain 1 (DYNC1I1 Human)
   

   Tags

   BioID and Flag-3x

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXTK024 [Origin_RSF1010_PartType1]

   Plasmid

   #229261

   Purpose

   XanthoMoClo Golden Gate Part Plasmid for cloning, contains Origin_RSF1010_PartType1

   Depositor

   Michael Springer

   Article

   Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

   Insert

   RSF1010 Origin
   

   Expression

   Bacterial

   Available Since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ubi:ccdb + mcherry

   Plasmid

   #233303

   Purpose

   GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of ccdb selection toxin and mCherry marker

   Depositor

   Thomas Baum

   Insert

   Ubi:ccdb + mcherry
   

   Use

   Gateway destination

   Available Since

   March 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Ubi:ccdb-3xHA + mcherry

   Plasmid

   #233302

   Purpose

   GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of 3xHA tag paired with ccdb selection toxin and mCherry marker

   Depositor

   Thomas Baum

   Insert

   Ubi:ccdb-3xHA + mcherry
   

   Use

   Gateway destination

   Tags

   3xHA

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ubi:ccdb-eGFP + mcherry

   Plasmid

   #233300

   Purpose

   GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of eGFP paired with ccdb selection toxin and mCherry marker

   Depositor

   Thomas Baum

   Insert

   Ubi:ccdb-eGFP + mcherry
   

   Use

   Gateway destination

   Tags

   eGFP

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. AiP12286 - pAAV-AiE0078m-minBG-iCre-WPRE-HGHpA (Alias: CN2286)

   Plasmid

   #230360

   Purpose

   AiE0078m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

   Depositor

   Allen Institute for Brain Science, Jonathan Ting

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   iCre
   

   Use

   AAV

   Promoter

   minBG

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. sgPPP1R2

    Plasmid

    #227391

    Purpose

    Adhesion module, guide RNA recognizes PPP1R2

    Depositor

    Clifford Brangwynne

    Article

    Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

    Insert

    guide RNA targeting PPP1R2
    

    Use

    Lentiviral

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pACYC-yhaM-RFP

    Plasmid

    #207651

    Purpose

    Overexpression of native L-cysteine desulfidase and RFP in E. coli MG1655

    Depositor

    Benjamin Woolston

    Article

    Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

    Insert

    yhaM
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUdOm7.1

    Plasmid

    #210287

    Purpose

    Empty backbone with zeocin resistance for high expression in mammalian cells

    Depositor

    Corrie daCosta

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMRS-dCas9

    Plasmid

    #220193

    Purpose

    mini-Tn7 dCas9 expression vector

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    Pseudomonas aeruginosa codon optimized

    Promoter

    promoterless

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGBW-m4046749

    Plasmid

    #145629

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp11

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp11 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pBBR1k-GFPuv

    Plasmid

    #106382

    Purpose

    IPTG-inducible trc promoter expressing gfpuv on BBR1 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. VinculinTS

    Plasmid

    #26019

    Depositor

    Martin Schwartz

    Article

    Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

    Insert

    Vinculin tension sensor
    

    Expression

    Mammalian

    Available Since

    Sept. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-EF1α-F-FLEX- Kir2.1-T2A-tdTomato

    Plasmid

    #60661

    Purpose

    Flp-dependent expression of Kir2.1-T2A-tdTomato

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Insert

    FLEX- Kir2.1-T2A-tdTomato (Kcnj2 Mouse)
    

    Use

    AAV

    Promoter

    EF1α

    Available Since

    Nov. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmiRFP720-N1

    Plasmid

    #136560

    Purpose

    A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP720

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    miRFP720
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. mito NAD+ biosensor

    Plasmid

    #186791

    Purpose

    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag totarget mitochondria

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    mito NAD+ biosensor
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Emerin pEGFP-C1 (637)

    Plasmid

    #61993

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    Emerin (EMD Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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