176,041 results

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tetO.Sox9.Puro

Plasmid

#117269

Purpose

Expresses Sox9 and puromycin resistance gene under control of tetO sequence.

Depositor

Henrik Ahlenius

Publication

Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

Insert

Sox9 (Sox9 Mouse)

Use

Lentiviral

Tags

T2A and puromycin resistant cassette (C terminal …

Expression

Mammalian

Promoter

tet-on

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pAAV-hSyn-mCherry

Plasmid

#114472

Purpose

mCherry Control

Depositor

Karl Deisseroth

Has service

AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

mCherry

Use

AAV

Promoter

hSyn

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

119 Articles

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lenti-sgRNA neo

Plasmid

#104992

Purpose

This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and G418 (neomycin) resistance from an EF-1a promoter.

Depositor

Brett Stringer

Publication

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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p11d-tRPA(123)

Plasmid

#102613

Purpose

Expression of human RPA in E. coli

Depositor

Marc Wold

Publication

Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

Inserts

RPA1 (RPA1 Human)
RPA2 (RPA2 Human)
RPA3 (RPA3 Human)

Expression

Bacterial

Promoter

T7

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pAAV-FLEX-EGFPL10a

Plasmid

#98747

Purpose

Cre-dependently expresses the fusion protein EGFPL10a

Depositor

Nathaniel Heintz, Alexander Nectow, Eric Schmidt

Publication

Nectow et al Cell Rep. 2017 Apr 18;19(3):655-667. doi

Has service

AAV Retrograde and AAV5

Insert

EGFPL10a

Use

AAV and Cre/Lox

Tags

GFP

Expression

Mammalian

Promoter

EF1a

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pAAV-nEFCas9

Plasmid

#87115

Purpose

SpCas9 expression AAV backbone plasmid under control of nEF promoter

Depositor

Juan Belmonte

Publication

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

nEF-Cas9

Use

AAV and CRISPR

Tags

HA-NLS

Expression

Mammalian

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pBA439

Plasmid

#85967

Purpose

Perturb-seq vector backbone

Depositor

Jonathan Weissman

Publication

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

sgGFP-NT2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pAAV-mDlx-GCaMP6f-Fishell-2

Plasmid

#83899

Purpose

GCaMP6f expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

Depositor

Gordon Fishell

Publication

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has service

AAV Retrograde, AAV1, and AAV9

Insert

GCaMP6f

Use

AAV

Expression

Mammalian

Promoter

mDlx

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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pET30-2-GAPDH

Plasmid

#83910

Purpose

stable overexpression

Depositor

David Sabatini

Publication

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

glyceraldehyde-3-phosphate dehydrogenase (GAPDH Human)

Tags

6x His tags

Expression

Bacterial

Promoter

T7

Available since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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IMS-APEX2

Plasmid

#79058

Purpose

Expresses APEX in the mitochondrial intermembrane space.

Depositor

Alice Ting

Publication

Hung et al Mol Cell. 2014 Jul 17;55(2):332-41. doi:

Insert

APEX

Tags

V5

Expression

Bacterial and Mammalian

Promoter

T7 promoter

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCDH-EF1

Plasmid

#72266

Purpose

Express gene of interest under EF-1 promoter

Depositor

Kazuhiro Oka

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Jan. 11, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

20 Articles

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pCS6-SMASh-YFP

Plasmid

#68852

Purpose

Expresses SMASh tag fused to YFP at N terminus in mammalian cells

Depositor

Michael Lin

Publication

Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

Insert

SMASh-YFP

Tags

SMASh tag

Expression

Mammalian

Promoter

CMV

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pComb3XTT

Plasmid

#63891

Purpose

Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)

Depositor

Carlos Barbas

Publication

Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

Insert

TT Fab

Use

Phage display

Tags

6xHis, HA tag, and geneIII

Expression

Bacterial

Promoter

lacZ

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pmax pona 12xTRICK 24xMS2SL

Plasmid

#64542

Purpose

TRICK translation biosensor with 12x copies of modified PP7 stem loops and 24x MS2 stem loops.

Depositor

Jeffrey Chao

Publication

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

12x PP7 stem-loops

Tags

24xMS2 stem loops, HA, NLS, and dNP799

Expression

Mammalian

Promoter

ponA

Available since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pT3-EF1a-Bmi1

Plasmid

#31783

Depositor

Xin Chen

Publication

Xu et al Mol Cancer Res. 2009 Dec;7(12):1937-45.

Insert

Bmi1 (BMI1 Human)

Tags

HA

Expression

Mammalian

Promoter

EF1alpha

Available since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pRRL SFFV d20GFP.T2A.mTagBFP Donor

Plasmid

#31485

Depositor

Andrew Scharenberg

Publication

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV d20BFP T2A BFP

Use

Lentiviral

Mutation

GFP has 20 amino acids deleted from the C terminu…

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pRsetB-pHRed

Plasmid

#31472

Depositor

Gary Yellen

Publication

Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

Insert

pHRed

Tags

7xHis

Expression

Bacterial

Available since

July 21, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCS2 HRS-RFP

Plasmid

#29685

Depositor

Edward De Robertis

Publication

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

hepatocyte growth factor-regulated tyrosine kinase substrate (HGS Human)

Tags

RFP

Expression

Mammalian

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pCMV-HA-JMJD2B

Plasmid

#24181

Purpose

Mammalian expression of human JMJD2B with HA tag

Depositor

Kristian Helin

Publication

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

JMJD2B (KDM4B Human)

Tags

HA

Expression

Mammalian

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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RFP-zyxin

Plasmid

#26720

Depositor

Anna Huttenlocher

Publication

Bhatt et al J Cell Sci. 2002 Sep 1. 115(Pt 17):3415-

Insert

Zyxin (ZYX Human)

Tags

DsRed

Expression

Bacterial and Mammalian

Available since

Dec. 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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