Improved designs for pET expression plasmids increase protein production yield in Escherichia coli.
Shilling PJ, Mirzadeh K, Cumming AJ, Widesheim M, Kock Z, Daley DO
Commun Biol. 2020 May 7;3(1):214. doi: 10.1038/s42003-020-0939-8. PubMed Article
Shilling PJ, Mirzadeh K, Cumming AJ, Widesheim M, Kock Z, Daley DO
Commun Biol. 2020 May 7;3(1):214. doi: 10.1038/s42003-020-0939-8. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 154462 | pET28a T7pCONS sfGFP | pET28a with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFP |
| 154463 | pET28a T7pCONS TIR-1 sfGFP | pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP |
| 154464 | pET28a T7pCONS TIR-2 sfGFP | pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP |
| 154467 | pET15b T7pCONS sfGFP | pET15b with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFP |
| 154468 | pET15b T7pCONS TIR-2 sfGFP | pET15b with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP |