163,606 results

1. pBS1C

   Plasmid

   #55168

   Purpose

   Empty vector, integration at amyE, ampr, cmr

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology; Bacillus biobrick box

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. mScarletI3-6DG5-MAPPER

   Plasmid

   #222421

   Purpose

   To express a label of ER-plasma membrane contacts based on GFP-MAPPER (#117721), but with mScarletI3 tag and replacing FRB with a protein of similar structure and sequence length (Neoleukin-2/15)

   Depositor

   Stephen Royle

   Article

   Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

   Insert

   MAPPER
   

   Use

   Tags

   mScarletI3-6DG5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6_empty-sgRNA(PP7)

   Plasmid

   #232432

   Purpose

   empty gRNA backbone which contains the PP7 aptamer in the scaffold tetraloop

   Depositor

   Gil Westmeyer

   Article

   Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

   Insert

   PP7-tagged sgRNA scaffold driven by human U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBR322-PVCpnf17-22

   Plasmid

   #198272

   Purpose

   Unmodified pPayload; contains WT PVC payloads/regulatory genes

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf17-22
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBB540

   Plasmid

   #27393

   Depositor

   Bernd Bukau

   Article

   de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

   Insert

   grpE, clpB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMax_GFP

   Plasmid

   #177825

   Purpose

   Host Cell Reactivation (HCR) control GFP plasmid (pMax backbone)

   Depositor

   Zachary Nagel, Leona Samson

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hsyn-NE2h

   Plasmid

   #208687

   Purpose

   Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h in neurons

   Depositor

   Yulong Li

   Has Service

   AAV5

   Insert

   GPCR activation based norepinephrine (NE) sensor GRAB_NE2h
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-FLEXfrt-EGFP-KASH

   Plasmid

   #154374

   Purpose

   Vector for Flp-dependent expression of EGFP tagged KASH protein domain

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   EGFP-KASH
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-EF1a-CRISPRoffv2.1-IRES-BlastR

   Plasmid

   #207180

   Purpose

   Lentiviral Expression Plasmid for CRISPRoff2.1 driven by EF1a Promoter

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Insert

   CRISPRoffv2.1
   

   Use

   CRISPR and Lentiviral

   Tags

   HA and TagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ9824: CMV-SV40_NLS-CasMINI-V3.1--3xFlag- c-Myc_NLS-polyA

    Plasmid

    #176271

    Purpose

    Expression of CasMINI-V3.1 in mammalian cells.

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    CasMINI-V3.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. EGFP-Tubulin-6

    Plasmid

    #56450

    Purpose

    Localization: Microtubules, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Tubulin (TUBA1B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. LSB-hsa-let-7f-5p

    Plasmid

    #103156

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-let-7f-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-let-7f-5p target
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. LSB-hsa-let-7f-1-3p

    Plasmid

    #103154

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-let-7f-1-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-let-7f-1-3p target (MIRLET7F1 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. MI-Luciferase-IRES-mCherry

    Plasmid

    #75020

    Purpose

    Encodes the expression of Luciferase and mCherry

    Depositor

    Xiaoping Sun

    Article

    Mallampati et al Blood. 2014 Jun 26;123(26):4064-76. doi:

    Inserts

    firefly Luciferase
    mCherry
    

    Use

    Luciferase and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCherry2-C1

    Plasmid

    #54563

    Purpose

    Localization: C1 Cloning Vector, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-Dio-ASAP5-Kv2.1-WPRE

    Plasmid

    #225708

    Purpose

    Cre dependent soma-targeted expression of the genetically encoded voltage indicator ASAP5

    Depositor

    Michael Lin

    Article

    Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

    Has Service

    AAV9

    Insert

    ASAP5-Kv2.1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1α

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBB542

    Plasmid

    #27395

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

    Insert

    dnaK, dnaJ, groESL
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3 Flag beta-1-adrenergic-receptor

    Plasmid

    #14698

    Depositor

    Robert Lefkowitz

    Article

    Tang et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

    Insert

    B1AR (ADRB1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMK411 (OsTIR1(F74G) mAID-EGFP-Nluc)

    Plasmid

    #140659

    Purpose

    OsTIR1(F74G)-P2A-mAID-EGFP-Nluc

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    OsTIR1(F74G) mAID-EGFP-Nluc TOL2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAS9-mCherry-Frame +2

    Plasmid

    #66941

    Purpose

    CRISPaint frame selector +2

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA frame +2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge