159,371 results

1. PKC alpha WT

   Plasmid

   #21232

   Depositor

   Bernard Weinstein

   Article

   Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

   Insert

   PKC alpha (PRKCA Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. P1-EYFP-pA (Construct 5)

   Plasmid

   #55197

   Purpose

   Synthetic promoter responsive to gRNA1

   Depositor

   Timothy Lu

   Article

   Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

   Insert

   EYFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Minimal Ade MLP

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pNC01

   Plasmid

   #233643

   Purpose

   For bacterial expression of sfGFP c-terminally tagged with VitelloTagA.

   Depositor

   Zak Swartz

   Article

   Clarke et al Development. 2024 Oct 15;151(20):dev2028

   Insert

   sfGFP with c-terminal VitelloTagA
   

   Use

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAK004

   Plasmid

   #233641

   Purpose

   For bacterial expression of eSpCas9(1.1) c-terminally tagged with VitelloTagA.

   Depositor

   Zak Swartz

   Article

   Clarke et al Development. 2024 Oct 15;151(20):dev2028

   Insert

   eSpCas9(1.1) with c-terminal VitelloTagA
   

   Use

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EMTB-mNeonGreen

   Plasmid

   #137802

   Purpose

   Visualization of microtubules

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv 160374

   Insert

   Ensconsin microtubule binding domain
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCS2+IMS-HyPer7

   Plasmid

   #136469

   Purpose

   Mammalian expression of mitochondrial intermembrane space targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Use

   Tags

   SMAC/DIABLO

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHDR_DDX4-T2A-tdTomato-puroTK

   Plasmid

   #222519

   Purpose

   Homology directed repair template for knocking in tdTomato reporter to DDX4.

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   tdTomato
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEGFP-C1-G3BP1-WT

   Plasmid

   #135997

   Purpose

   WT G3BP1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   G3BP1 (G3BP1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-CreERT2

   Plasmid

   #14797

   Purpose

   Mammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   Cre-ERT2 fusion protein
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Tet-on circRNA Expression Vector

    Plasmid

    #92351

    Purpose

    The vector can ef_ciently produce a circRNA (circmCherry) from a split EGFP gene after Dox treatment.

    Depositor

    Ling-Ling Chen

    Article

    Zhang et al Cell Rep. 2016 Apr 19;15(3):611-24. doi:

    Insert

    mCherry and split EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTet

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. H2B-GFP

    Plasmid

    #11680

    Depositor

    Geoff Wahl

    Article

    Kanda et al Curr Biol. 1998 Mar 26. 8(7):377-85.

    Insert

    Human histone H2B (H2BC11 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 31, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. UBC plasmid

    Plasmid

    #169746

    Purpose

    Unregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV_BE4max_P2A_GFP

    Plasmid

    #112099

    Purpose

    C:G-to-T:A base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    BE4max_P2A_GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUCM-AAVS1-TO-hNGN2

    Plasmid

    #105840

    Purpose

    Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron

    Depositor

    Michael Ward

    Article

    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

    Inserts

    Neurogenin 2 (NEUROG2 Human)
    mCherry
    rtTA3G
    

    Use

    CRISPR and TALEN

    Tags

    Expression

    Mutation

    Promoter

    CAG, EF-1alpha, and TRE3G

    Available since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pT3TS-RfxCas13d-NLS-HA

    Plasmid

    #141321

    Purpose

    Plasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)

    Depositor

    Ariel Bazzini, Miguel Angel Moreno-Mateos

    Article

    Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

    Insert

    Rfx-Cas13d (NLS-RfxCas13d-NLS)
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby

    Plasmid

    #71760

    Purpose

    mGFP and Synaptophysin-mRuby expression from Cre-expressing neurons

    Depositor

    Liqun Luo

    Article

    Beier et al Cell. 2015 Jul 30;162(3):622-34. doi: 10

    Has Service

    AAV1

    Inserts

    mGFP
    Synaptophysin
    

    Use

    AAV and Cre/Lox

    Tags

    Palmitoylation signal and mRuby

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mtsCOXVIIIx2-B-gTEMP/pcDNA3

    Plasmid

    #199806

    Purpose

    Fluorescent temperature indicator B-gTEMP fused with mitochondrial targeting sequence

    Depositor

    Takeharu Nagai

    Article

    Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

    Insert

    B-gTEMP
    

    Use

    Tags

    mtsCOXVIIIx2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMT525

    Plasmid

    #131392

    Purpose

    L1RP retrotransposition reporter

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Insert

    L1RP GFP-AI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE-BRAF-V600E

    Plasmid

    #116204

    Purpose

    Lentiviral expression of BRAF V600E

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    BRAF (BRAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    V600E

    Promoter

    Available since

    Jan. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pH-ePPE

    Plasmid

    #183097

    Purpose

    For plant prime editing in rice plants

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

    Insert

    Nls-nCas9-NC-nls-MLV-Nls
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A in SpCas9

    Promoter

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge