165,775 results

1. TadCBE-Cas9NG

   Plasmid

   #225093

   Purpose

   Plasmid for bacterial purification of codon-optimized TadCBE-Cas9NG

   Depositor

   Vijay Sankaran

   Article

   Martin-Rufino et al Science. 2025 Apr 4;388(6742):52-59. doi

   Insert

   TadCBE-Cas9NG
   

   Use

   CRISPR

   Tags

   8xHis

   Expression

   Bacterial

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJYS1Peftu

   Plasmid

   #85546

   Purpose

   Constitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter

   Depositor

   Sheng Yang

   Article

   Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

   Inserts

   Cpf1
   recT
   

   Use

   CRISPR; Shuttle vector corynebacterium glutamicum…

   Expression

   Bacterial

   Promoter

   etfu and unknown

   Available Since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-TORCAR

   Plasmid

   #64927

   Purpose

   FRET-based biosensor for monitoring mTORC1 kinase activity.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   TORCAR (EIF4EBP1 Synthetic, Human)
   

   Tags

   Cerulean and YPet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pINDUCER dCas9-TET1CD

   Plasmid

   #101921

   Purpose

   The dCas9-TET1CD fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948).

   Depositor

   Danwei Huangfu

   Article

   Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

   Insert

   dCas9-TET1CD
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLifeAct-miRFP670nano

   Plasmid

   #127434

   Purpose

   F-Actin labeling with near-infrared fluorescent protein miRFP670nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

   Insert

   LifeAct-miRFP670nano
   

   Tags

   miRFP670nano

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMBP-LbCas12a

   Plasmid

   #113431

   Purpose

   Expresses 10xHis-MBP fused to LbCas12a

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Has Service

   Cloning Grade DNA

   Insert

   LbCas12a
   

   Tags

   MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. ER-GCaMP6f

   Plasmid

   #182548

   Purpose

   Expresses GCaMP6f on the cytosolic side of the ER (P-450site)

   Depositor

   Chris Richards

   Article

   Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

   Insert

   ER localized P450
   

   Expression

   Bacterial and Mammalian

   Available Since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330S-4

   Plasmid

   #58780

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-CMV-Blast-PIP-FUCCI

   Plasmid

   #138715

   Purpose

   Lentiviral expression of the cell cycle marker PIP-FUCCI

   Depositor

   Jean Cook

   Article

   Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

   Insert

   PIP-FUCCI
   

   Use

   Lentiviral

   Tags

   mCherry and mVenus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJAF205

    Plasmid

    #60407

    Purpose

    Flag-tagged full length GMAP210/Trip11

    Depositor

    Gregory Pazour

    Article

    Follit et al PLoS Genet. 2008 Dec;4(12):e1000315. doi

    Insert

    GMAP210 (Trip11 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

11. YUC4:RUBY

    Plasmid

    #160907

    Purpose

    RUBY under the control of YUC4 promoter/tissue specific marker for gene expression

    Depositor

    Yunde Zhao

    Article

    He et al Hortic Res. 2020 Sep 19;7:152. doi: 10.1

    Insert

    RUBY and YUC4 promoter
    

    Expression

    Plant

    Promoter

    YUC4

    Available Since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV_3XHA-ATP6V1B2

    Plasmid

    #228864

    Purpose

    Stable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA

    Depositor

    Wilhelm Palm

    Article

    Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

    Insert

    ATP6V1B2 (ATP6V1B2 Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Syn-eGFP-2A-mCherry_D4H

    Plasmid

    #194875

    Purpose

    Ratiometric D4H sensor for in vivo neuron-specific cholesterol visualization

    Depositor

    Peter Scheiffele

    Article

    Czernecki et al bioRxiv 2022.11.16.516849

    Insert

    GFP-T2A-mCherry-D4H
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    human Synapsin

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1 - TRC control

    Plasmid

    #10879

    Purpose

    Negative control shRNA vector containing non-hairpin insert.

    Depositor

    David Root

    Article

    Moffat et al Cell. 2006 Mar 24. 124(6):1283-98.

    Insert

    non-hairpin 18bp
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. EF1a_FOXA2_P2A_Hygro_Barcode

    Plasmid

    #120439

    Purpose

    Barcoded lentiviral vector to express FOXA2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    FOXA2 (FOXA2 Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Flag::UbvG08 I44A, deltaGG

    Plasmid

    #74939

    Purpose

    Ubiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53). Blocks 53BP1 from accumulating at sites of DNA damage. Potent selective inhibitor of 53BP1

    Depositor

    Daniel Durocher

    Article

    Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

    Insert

    Ubiquitin
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    UbvG08 with I44A mutation and no terminal Glycines

    Promoter

    CMV

    Available Since

    June 30, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pWM_12x601_25bpLinker

    Plasmid

    #157788

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 25 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_25bp linker
    

    Expression

    Bacterial

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLX304 CMV HA-HaloTag-FRB-sTurboID (C)

    Plasmid

    #153003

    Purpose

    Expresses low-affinity split-TurboID C-terminal fragment in mammalian cells

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    sTurboID (C) (G74)
    

    Use

    Lentiviral

    Tags

    FRB, HA, and HaloTag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBabe_Zeo_cOVA_T2A_mStrawberry

    Plasmid

    #161736

    Purpose

    Expression of cOVA and mStrawberry

    Depositor

    Alec Kimmelman

    Article

    Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

    Insert

    cytoplasmic ovalbumin
    

    Use

    Retroviral

    Tags

    mStrawberry

    Expression

    Mammalian

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT3-N90-beta-catenin

    Plasmid

    #31785

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    beta-catenin (CTNNB1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Deletion of aa 1-90- Constitutitvely Active

    Promoter

    EF1alpha

    Available Since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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