163,606 results

1. pLentiRNACRISPR_008-TetO-NLS-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

   Plasmid

   #223173

   Purpose

   Expresses hfCas13d (N2V8) in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus.

   Depositor

   Neville Sanjana

   Article

   Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

   Insert

   hfCas13d (N2V8)
   

   Use

   Lentiviral

   Tags

   NLS-HA

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19 Giles integrase

   Plasmid

   #170049

   Purpose

   pUC19 with Giles integrase

   Depositor

   Clifton Barry III

   Article

   Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

   Insert

   GIles Integrase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-ATF4 mScarlet NLS

   Plasmid

   #115969

   Purpose

   ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I

   Depositor

   David Andrews

   Article

   Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

   Insert

   activating transcription factor 4 (ATF4 Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   HA tag, c-myc NLS, and mScarlet-I

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pdCas9-bacteria

   Plasmid

   #44249

   Purpose

   aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   dCas9 (bacteria)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   pLtetO-1

   Available Since

   April 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pB-CuR-hSOD1

   Plasmid

   #232477

   Purpose

   The inducible PiggyBac Cumate Switch vector (PBQM812A-1 System Biosciences) expressing human SOD1gene including flag -tag (DYKDDDDK) on the 3' end

   Depositor

   Lukas Trantirek

   Article

   Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

   Insert

   Homo sapiens superoxide dismutase 1 (SOD1 Human)
   

   Use

   Transposon system

   Tags

   DYKDDDDK (FLAG-tag)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-CuO

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. OpenCRISPR-1 sgRNA-16nt stem

   Plasmid

   #221566

   Purpose

   Express OpenCRISPR-1 sgRNA (16nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

   Depositor

   Ali Madani

   Article

   Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

   Insert

   OpenCRISPR-1 sgRNA 16nt stem loop
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Aug. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pOpen-MMLV_RT (lack H)

   Plasmid

   #165556

   Purpose

   Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. Comparable to SuperScriptII from Thermo Fisher.

   Depositor

   FreeGenes Project, Drew Endy, Jennifer Molloy

   Insert

   Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 16, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. CMV500 empty vector

   Plasmid

   #33348

   Depositor

   Charles Vinson

   Article

   Rishi et al J Biol Chem. 2004 Mar 19;279(12):11863-7

   Type

   Empty backbone

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMRX-IP-GFP-LC3-RFP

   Plasmid

   #84573

   Purpose

   Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Retroviral

   Tags

   EGFP and mRFP1

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX307 Luciferase

    Plasmid

    #117734

    Purpose

    Open reading frame vector encoding Luciferase

    Depositor

    William Hahn

    Article

    Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

    Insert

    firefly luciferase
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYL192 (TRV1)

    Plasmid

    #148968

    Purpose

    Tobacco Rattle Virus RNA 1; used for virus induced gene silencing in plants along with pYL156 (TRV RNA2)

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Liu et al Plant J. 2002 May;30(4):415-29. doi: 10.

    Insert

    TRV RNA1
    

    Use

    Plant vector with trv rna1; this vector used with…

    Tags

    Expression

    Mutation

    P697S mutation in putative replicase. S239G and S…

    Promoter

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB-cT3G-cERP2-RUNX2

    Plasmid

    #192926

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of RUNX2

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    RUNX2 (RUNX2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSN837

    Plasmid

    #184825

    Purpose

    Expresses human KIF5A in mammalian cells

    Depositor

    Kyoko Chiba

    Article

    Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

    Insert

    KIF5A (KIF5A Human)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3 Flag beta-2-adrenergic-receptor

    Plasmid

    #14697

    Depositor

    Robert Lefkowitz

    Article

    Tang et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

    Insert

    B2AR (ADRB2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. HA-HIF2alpha-P405A/P531A-pcDNA3

    Plasmid

    #18956

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 405 to Alanin…

    Promoter

    Available Since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSIRV-NF-kB-eGFP

    Plasmid

    #118093

    Purpose

    Fluorescent reporter for NF-kB activity

    Depositor

    Peter Steinberger

    Article

    Jutz et al Oncotarget. 2017 May 4;8(39):64892-64906

    Insert

    eGFP
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    minimal promoter with NF-kB responsive element

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiFUCCI(CA)5

    Plasmid

    #223176

    Purpose

    Lentiviral fluorescent ubiquitination-based cell cycle indicator (FUCCI)

    Depositor

    Neville Sanjana

    Article

    Liang et al Cell. 2024 Dec 26;187(26):7637-7654.e29.

    Insert

    FUCCI(CA)5
    

    Use

    Lentiviral

    Tags

    AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLX_lenti_osTir1_9Myc_P2A_Bsd

    Plasmid

    #139746

    Purpose

    lentiviral vector expressing rice E3 ligase Tir1 in mammalian cells with selection marker

    Depositor

    Robert Tjian

    Article

    Xie et al Nat Methods. 2020 Apr;17(4):430-436. doi

    Insert

    rice E3 ligase Tir1 (osTir1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. phMYT1L-N174

    Plasmid

    #66809

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses human MYT1L with G418 resistance

    Depositor

    Andrew Yoo

    Article

    Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

    Insert

    MYT1L (MYT1L Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1a

    Available Since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. ATeam1.03-nD/nA/pcDNA3

    Plasmid

    #51958

    Purpose

    mammalian expression of ATeam1.03-nD/nA, a FRET-based ATP indicator

    Depositor

    Takeharu Nagai

    Article

    Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

    Insert

    ATeam1.03-nD/nA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge