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Plasmid#175358PurposeMammalian expression vector for over-expression of anti-FLAG M2 light chainDepositorInsertanti-FLAG M2 light chain
UseTagsN-terminal secretion signal peptide (MEAPAQLLFLLL…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_22A
Plasmid#91133PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt IIDepositorInsertEngineering Reagent: 35S:AtCas9 + AtU6:gRNA
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cas9-GFP
Plasmid#86145PurposeLentiviral vector expressing Cas9-GFPDepositorInsertCas9-GFP
UseCRISPR and LentiviralTagsFLAG and GFPExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Cre-3xmiR122-WPRE-HGHpA
Plasmid#183776PurposeAAV genome with a CAG driven Cre recombinase with mR122 target sequence repeats to reduce transgene expression in hepatocytes.DepositorInsertCre
UseAAVTagsExpressionMutationNAPromoterCAGAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57-MCS1-P2A-MCS2 (Neo)
Plasmid#89180PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterTREAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX_EFS-Cre_ppt-del
Plasmid#162073PurposeCompact Cre expression vector with modified PPT designed to generate high-titer non-integrating lentivirus when packaged with PsPax2-D64V (Addgene #63586)DepositorInsertCre
UseCre/Lox and LentiviralTagsExpressionMammalianMutationcodon-optimized for expression in M. musculusPromoterEFS (part of insert)Available sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
anti-FLAG M2 heavy chain
Plasmid#175359PurposeMammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)DepositorInsertanti-FLAG M2 heavy chain
UseTagsHis8 tag and N-terminal secretion signal peptide …ExpressionMammalianMutationL51I (see paper)PromoterCMVAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_EGFPligand
Plasmid#79129PurposeLentiviral vector for surface expressed EGFP (SFFV promoter)DepositorInsertEGFP surface ligand
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHEN2-SG4 R105A
Plasmid#196072PurposeExpress mutant (R105A) SG4 in E.coliDepositorInsertSG4 R105A
UseTags3XFLAG and 6XHisExpressionBacterialMutationR105A mutation in SG4PromoterT7Available sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-jGCaMP8s-WPRE
Plasmid#179256PurposeAAV-mediated expression of GCaMP8s under the CAG promoterDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8s
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6a:sgRNA#1
Plasmid#64245Purposeexpresses sgRNA under U6a promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
hPU.1ΔN165-pET28b
Plasmid#199796PurposeExpressed human PU.1/Spi-1 ETS domain (residues 165 to 270) in E. coliDepositorInsertspi1 (SPI1 Human)
UseTagsNoneExpressionBacterialMutationPromoterAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
mRFP-Rab7
Plasmid#14436PurposeMammalian expression of Rab7 fused to mRFP. Used for localization to the late endosome, and other early endosomal vacuoles.DepositorInsertRab 7 (RAB7A Canis familialis)
UseTagsmRFPExpressionMammalianMutation3 silent mutations near the C-terminusPromoterAvailable sinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
EGFP-E-Syt1
Plasmid#66830PurposeMammalian Expression, Bacterial ExpressionDepositorInsertE-Syt1 (ESYT1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
human p53-(1-393)
Plasmid#24859DepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationFull length.PromoterAvailable sinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
GAL4BD-VP16/pDON207
Plasmid#201230PurposeGAL4-VP16 transcription factorDepositorInsertGAL4BD-VP16
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
sUPRa
Plasmid#223242PurposesUPRa is a dual promoter and two-color construct, which is designed to report the global unfolded protein response (UPR) and affords unbiased cell detection irrespective of UPR levelsDepositorInsertsUPRa
UseAAVTagsExpressionMutationPromoterCustomAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-pHluorin_M153R-CD63-mScarlet
Plasmid#172118PurposeDual-color fluorescent reporter for CD63-positive exosome secretion and uptakeDepositorInsertCD63 (CD63 Human)
UseLentiviralTagsmScarlet and pHluorin_M153R in a small extracellu…ExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1A-MYC-IRES-luc
Plasmid#129775PurposeTransposon based vector that expresses human MYC followed by IRES and firefly luciferaseDepositorInserthuman MYC (MYC Human)
UseTagsluciferaseExpressionMammalianMutationPromoterEF1AAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
35S-VP16-GWY/pAM
Plasmid#201234PurposeUsed to produce and express in planta C-terminal fusion proteins with the VP16 activation domainDepositorInsert35S-VP16-GWY
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only