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shh10

Plasmid

#64867

Purpose

This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.

Depositor

John Flannery, David Schaffer

Article

Klimczak et al PLoS One. 2009 Oct 14;4(10):e7467. doi:

Insert

shh10 cap

Use

AAV

Mutation

I319V, N451D, D532N

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pDESTtol2pACrymCherry

Plasmid

#64023

Purpose

Gateway destination vector with zebrafish aA-crystallin promoter driving mCherry GFP in lens

Depositor

Joachim Berger, Peter Currie

Article

Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

Insert

mCherry

Use

Gateway Cloning

Promoter

alpha-Acrystallin promoter

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pSaCas9_GFP

Plasmid

#64709

Purpose

Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells

Depositor

Kiran Musunuru

Insert

SaCas9-2A-GFP

Use

CRISPR

Tags

2A-GFP and 3xFLAG-SV40 NLS

Expression

Mammalian

Promoter

CAG

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti.PGK.LifeAct-Ruby.W

Plasmid

#51009

Purpose

Lentivector that expresses LifeAct-Ruby from internal human PGK promoter

Depositor

Rusty Lansford

Insert

LifeAct-mRFPruby

Use

Lentiviral

Tags

LifeAct

Expression

Mammalian

Promoter

human PGK

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-CFP II (pMIC II)

Plasmid

#52109

Purpose

a bicistronic IRES-CFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

Depositor

Dario Vignali

Article

Holst et al Nat Protoc. 2006;1(1):406-17.

Type

Empty backbone

Use

Retroviral

Tags

IRES-ECFP

Expression

Mammalian

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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mcherry-PH

Plasmid

#36075

Depositor

Narasimhan Gautam

Article

Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

Insert

PH domain of PLCdelta1 (PLCD1 Human)

Tags

mcherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Myc-DNMT3L

Plasmid

#35523

Depositor

Arthur Riggs

Article

Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

Insert

DNMT3L (DNMT3L Human)

Tags

Myc

Expression

Mammalian

Available Since

March 9, 2012

Availability

Academic Institutions and Nonprofits only

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FLAG-HSF1

Plasmid

#32537

Depositor

Stuart Calderwood

Article

Wang et al Mol Cell Biol. 2003 Sep;23(17):6013-26.

Insert

HSF1 (HSF1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

see depositor comments below

Promoter

CMV

Available Since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only

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p6344 pcDNA4-TO-HA-Brd4FL

Plasmid

#31351

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

Brd4FL

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-hMcl-1

Plasmid

#25375

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

MCL1 (MCL1 Human)

Tags

V5 and His tag

Expression

Mammalian

Available Since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-JAK1

Plasmid

#23932

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

JAK1 (JAK1 Human)

Use

Gateway Cloning

Mutation

R647H (numbering relative to NP_002218.1)

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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Myc tag WT PTB

Plasmid

#23024

Depositor

Douglas Black

Article

Xie et al Proc Natl Acad Sci U S A. 2003 Jul 22. 1

Insert

PTB1 (PTBP1 Human)

Tags

Myc

Expression

Mammalian

Available Since

June 4, 2010

Availability

Academic Institutions and Nonprofits only
pEGFP C3-Lats1

Plasmid

#19053

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Large tumor suppressor kinase 1, N mutant (LATS1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

**

Available Since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only

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IRES-puro-GFP

Plasmid

#16616

Depositor

Bert Vogelstein

Article

Torrance et al Nat Biotechnol. 2001 Oct . 19(10):940-5.

Insert

GFP

Expression

Mammalian

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

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M57 pPET28a-TEV-full-length human beta catenin

Plasmid

#17198

Depositor

Randall Moon

Insert

beta catenin (CTNNB1 Human)

Tags

HIS

Expression

Bacterial

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pSKDuet01

Plasmid

#12172

Depositor

Hideo Iwai

Article

Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

Insert

NpuDnaE

Tags

His-GB1

Expression

Bacterial

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

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pCS2-hDKK1-flag

Plasmid

#15494

Depositor

Sergei Sokol

Article

Krupnik et al Gene. 1999 Oct 1. 238(2):301-13.

Insert

DKK1 (DKK1 Human)

Tags

flag

Expression

Mammalian

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pEGFP hsp70 K71E

Plasmid

#15216

Depositor

Lois Greene

Article

Zeng et al J Cell Sci. 2004 Oct 1. 117(Pt 21):4991-

Insert

hsp70 K71E (HSPA1A Human)

Tags

EGFP

Expression

Mammalian

Mutation

K71E, ATPase-deficient mutant.

Available Since

June 15, 2007

Availability

Academic Institutions and Nonprofits only

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CD20

Plasmid

#1890

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

CD20 (MS4A1 Human)

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pMKO.1 puro hTERT shRNA

Plasmid

#10688

Depositor

William Hahn

Article

Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

Insert

hTERT shRNA (TERT Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

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