165,775 results

1. pCMV CEPIA4mt

   Plasmid

   #58220

   Purpose

   Green fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

   Depositor

   Masamitsu Iino

   Article

   Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

   Insert

   CEPIA4mt
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   GCaMP2 N263Y E282V E334D M339L F395W D436E

   Promoter

   CMV

   Available Since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. Pink Flamindo

   Plasmid

   #102356

   Purpose

   biosensor for cAMP in mammalian cells

   Depositor

   Tetsuya Kitaguchi

   Article

   Harada et al Sci Rep. 2017 Aug 4;7(1):7351. doi: 10.1

   Insert

   Pink Flamindo
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLQ10844

   Plasmid

   #183956

   Purpose

   CAG-hyperdCas12a-HA-miniVPR-mCherry-T2A-HygroR

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Insert

   HyperdCas12a
   

   Use

   CRISPR

   Tags

   HA, Hygromycin Resistance, and mCherry

   Expression

   Mammalian

   Mutation

   D832A, D156R, E292R, D235R, D350R

   Promoter

   CAG

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX601-mCherry

   Plasmid

   #84039

   Purpose

   Staphylococcus aureus (SaCas9) conjugated with mCherry

   Depositor

   Yuet Wai Kan

   Article

   Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

   Insert

   SaCas9
   

   Use

   AAV and CRISPR

   Tags

   NLS and T2A-mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. IFN-Beta_pGL3

   Plasmid

   #102597

   Purpose

   IFN-beta promoter driving luciferase. Use in IFN reporter assay.

   Depositor

   Nicolas Manel

   Article

   Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

   Insert

   Human IFN-Beta promoter (IFNB1 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Available Since

   Jan. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. mEos3.2-N1

   Plasmid

   #54525

   Purpose

   Localization: N1 Cloning Vector, Excitation: 507 / 572, Emission: 516 / 580

   Depositor

   Michael Davidson, Tao Xu

   Article

   Zhang et al Nat Methods. 2012 May 13;9(7):727-9. doi

   Type

   Empty backbone

   Tags

   mEos3.2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 12, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pcDNA3.1_nV5-AMOTL2

   Plasmid

   #172999

   Purpose

   constitutive expression of V5-tagged AMOTL2

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   V5-AMOTL2 (AMOTL2 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21b FAST-PETase

   Plasmid

   #225760

   Purpose

   Expresses codon-optimized FAST-PETase in E. coli with a C-terminal his-tag

   Depositor

   Jason Boock

   Article

   Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

   Insert

   FAST-PETase
   

   Tags

   6xHis Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. p6351 MSCV-CMV-CMV-Flag-HA-NSD3

   Plasmid

   #31357

   Depositor

   Peter Howley

   Article

   Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

   Insert

   NSD3 (NSD3 Human)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Available Since

   Oct. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-53-RtcB

    Plasmid

    #51282

    Purpose

    E. coli expression vector for E. coli RtcB RNA ligase

    Depositor

    Jay Hesselberth

    Article

    Peach et al Nucleic Acids Res. 2015 May 22. pii: gkv

    Insert

    E. coli RtcB
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7 RNA Polymerase

    Available Since

    March 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. Mfn2-YFP

    Plasmid

    #28010

    Depositor

    Richard Youle

    Article

    Karbowski et al J Cell Biol. 2002 Dec 23. 159(6):931-8.

    Insert

    mitofusin 2 (MFN2 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    silent S508S

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFGC-pcoCas9

    Plasmid

    #52256

    Purpose

    A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA

    Depositor

    Jen Sheen

    Insert

    35SPPDK:pcoCas9:NOS cassette
    

    Use

    CRISPR; Plant expression

    Promoter

    constitutive 35SPPDK promoter

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWPI-N1ICD

    Plasmid

    #185525

    Purpose

    Constitutive overexpression of human NOTCH1 intracellular domain

    Depositor

    Sean Palecek, Eric Shusta

    Article

    Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

    Insert

    NOTCH1 intracellular domain (NOTCH1 Human)
    

    Use

    Lentiviral

    Promoter

    EF-1a

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTRIP-SFFV-EGFP-NLS-FLAG-cGAS

    Plasmid

    #127659

    Purpose

    Over-express EGFP-NLS-FLAG-cGAS

    Depositor

    Nicolas Manel

    Article

    Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

    Insert

    cGAS (CGAS Human)
    

    Use

    Lentiviral

    Tags

    EGFP-NLS-Flag

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS-slpGFP

    Plasmid

    #27171

    Depositor

    Michela Lizier

    Article

    Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

    Insert

    surface (S)-layer protein promoter region fused with modified green fluorescent protein 5
    

    Expression

    Bacterial

    Available Since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBBR1_DVK_AE(J23100-BCD2-E0040m-B0015)

    Plasmid

    #197660

    Purpose

    Expresses GFP under the control of J23100 and BCD2

    Depositor

    Eric Young

    Article

    Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-Flag mTOR wt

    Plasmid

    #26603

    Purpose

    Mammalian expression of human flag-tagged mTor

    Depositor

    Jie Chen

    Article

    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

    Insert

    mTOR (MTOR Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Oct. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCLucf

    Plasmid

    #37328

    Depositor

    John Schiller

    Article

    Johnson et al J Virol. 2009 Mar;83(5):2067-74. Epub 20

    Inserts

    Luciferase (luciferase Fly)
    GFP
    

    Expression

    Mammalian

    Promoter

    CMV and SV40

    Available Since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMW#3

    Plasmid

    #13350

    Depositor

    Marian Walhout

    Article

    Deplancke et al Cell. 2006 Jun 16. 125(6):1193-205.

    Insert

    LacZ reporter
    

    Expression

    Yeast

    Available Since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-T7pol

    Plasmid

    #59926

    Purpose

    Expression vector for T7 RNA polymerase gene

    Depositor

    Ian Wickersham

    Insert

    T7 RNA polymerase
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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