176,041 results
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Plasmid#68072Purposemammalian expression of ER localized moxGFPDepositorInsertmoxGFP
TagsKDEL ER retention sequence and bovine prolactin s…ExpressionMammalianPromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]
Plasmid#62723PurposeAAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.DepositorHas serviceAAV1 and AAV5InsertChrimsonR-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationChrimson K176R mutantPromoterhuman synapsin promoterAvailable sinceApril 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAct-Cas9
Plasmid#62209PurposeExpresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster.DepositorInsertcas9
ExpressionInsectPromoteract5CAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENTR-ERKKTRClover
Plasmid#59138PurposeORF encoding ERK KTR mClover flanked by Gateway sequencesDepositorInsertERK Kinase Translocation Reporter (MAPK1 Mouse, Human)
Available sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMSCV-IRES-Blue FP
Plasmid#52115Purposea bicistronic IRES-Blue FP containing retroviral vectorDepositorTypeEmpty backboneUseRetroviralTagsIRES-Blue FPExpressionMammalianAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA
Plasmid#50943PurposeBicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.DepositorInsertmRuby2-P2A-GCaMP6f
UseAAVPromoterhSyn1Available sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDULE-ABK
Plasmid#49086PurposeA pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experimentsDepositorPublicationInsertstRNA synthetase
tRNA for a photocrosslinking amino acid
ExpressionBacterialMutationL274M, C313A, Y349FPromoterGlnRS and lppAvailable sinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV500 A-CREB
Plasmid#33371DepositorInsertCREB (Creb1 Mouse)
TagsFLAGExpressionMammalianMutationDominant Negative (see notes)PromoterCMVAvailable sinceDec. 19, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLM-mCitrine-Sox2
Plasmid#23242DepositorInsertmCitrine_2A_SOX2 (SOX2 Human, synthetic fluorescent protein)
UseLentiviralAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ChR2-2A-Halo
Plasmid#22047PurposeExpression of ChR2-GFP and Halo-YFP for bi-directional control of neuronal activityDepositorInsertChR2-GFP-2A-Halo-YFP
TagsGFP and YFPExpressionMammalianMutationNote that there is a S147R mut in YFP. This mutat…PromoterCMVAvailable sinceSept. 17, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA
Plasmid#20299PurposeCre-activated AAV expression of mCherryDepositorHas serviceAAV9InsertmCherry
UseAAVExpressionMammalianAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV6M-Pak1 K299R
Plasmid#12210DepositorPublicationAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLJM1-FIRE-pHLy
Plasmid#170775PurposeRatiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cellsDepositorInsertLAMP1 (LAMP1 Human)
UseLentiviralTagsmCherry and mTFP1ExpressionMammalianMutation84 nucleotide Signal Sequence switched to the beg…PromoterCMVAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLX304 CMV OMM-FKBP-V5-sTurboID (N)
Plasmid#153006PurposeExpresses split-TurboID N-terminal fragment targeted to outer mitochondrial membraneDepositorInsertOMM (Tom20)-sTurboID (N) (L73)
UseLentiviralTagsFKBP and V5ExpressionMammalianPromoterCMVAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR207 SARS-CoV-2 M
Plasmid#141274PurposeGateway-compatible Entry vector, with insert of the membrane (M) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorHas serviceCloning Grade DNAInsertM (M SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway CloningMutationMany synonymous changes due to codon optimizationAvailable sinceApril 1, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
ER-mScarletI
Plasmid#137805PurposeVisualization of the Endoplasmic ReticulumDepositorInsertKDEL
ExpressionMammalianPromoterCMVAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST-CMV-N-mCherry
Plasmid#123215PurposeN-terminal mCherry tag. Gateway destination vector for mammalian expression.DepositorTypeEmpty backboneUseGateway CloningTagsmCherryExpressionMammalianPromoterCMVAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST-CMV-C-EGFP
Plasmid#122844PurposeC-terminal EGFP tag. Gateway destination vector for mammalian expression.DepositorTypeEmpty backboneUseGateway CloningTagsEGFPExpressionMammalianPromoterCMVAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMSCV-Cas9-2A-GFP-sgRNA
Plasmid#124889PurposeContains spCas9-2A-GFP and guide RNA scaffold in single retroviral vectorDepositorInsertCas9
UseRetroviralTags2A-GFPPromoterMSCVAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLEM415-ldhL-mRFP1
Plasmid#99842PurposeExpression of RFP in LactobacillusDepositorInsertmonomeric red fluorescent protein
TagsFLAG/XpressExpressionBacterialPromoterldhlAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by