165,775 results

1. PET-HiFi SpCas9-NLS-6xHis

   Plasmid

   #207376

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   HiFi SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   R691A

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX459-sgAAVS1

   Plasmid

   #184403

   Purpose

   pX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGAT

   Depositor

   Robert Bradley

   Article

   Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

   Insert

   AAVS1-specific sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 promoter

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. px330-Pten-2

   Plasmid

   #162552

   Purpose

   sgRNA targeting Pten

   Depositor

   Amaia Lujambio

   Article

   Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

   Insert

   sgRNA targeting Pten (Pten Mouse)
   

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL-p21UTR

   Plasmid

   #20014

   Depositor

   Robert Blelloch

   Article

   Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

   Insert

   p21 3'UTR (Cdkn1a Mouse)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Aug. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

5. pAAV.CAG.iGluSnFR3.v857.PDGFR

   Plasmid

   #178333

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   pAAV CAG iGluSnFR3 v857.PDGFR
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

   Tags

   IgK-chain, Myc epi tag, and PDGFR TM Domain

   Expression

   Mammalian

   Available Since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. H2B-mNeonGreen-IRESpuro2

   Plasmid

   #183745

   Purpose

   Expresses H2B-mNeonGreen

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   H2B (H2BC16P Human)
   

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOSIP-KL-mcherry

   Plasmid

   #116947

   Purpose

   Integrating mCherry into lambda attB in E. coli chromosome.

   Depositor

   David Bikard

   Article

   Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

   Insert

   mcherry
   

   Available Since

   March 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX307-VHL(C162F)-3F-BirA

   Plasmid

   #220149

   Purpose

   Expresses VHL(C162F)-BirA as a control for VHL substrate identification by E-STUB

   Depositor

   William Sellers

   Article

   Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

   Insert

   von Hippel-Lindau tumor suppressor (VHL Human)
   

   Use

   Lentiviral

   Tags

   3F-BirA

   Expression

   Mammalian

   Mutation

   changed cysteine 162 to phenylalanine; codon opti…

   Promoter

   EF-1a

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAR028 Lenti pEF-dEnAsCas12a-NLS-ZNF705F KRAB-3XHA-T2A-BSD

   Plasmid

   #195545

   Purpose

   dCas12a effector fusion for manipulating transcription

   Depositor

   Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dEnAsCas12a-NLS-TopKRAB-3XHA-T2A-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   3XHA

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHCMV-EcoEnv

    Plasmid

    #15802

    Depositor

    Miguel Sena-Esteves

    Article

    Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

    Insert

    MLV env (ecotropic)
    

    Expression

    Mammalian

    Available Since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. Flag-TRIM28

    Plasmid

    #124960

    Purpose

    Mammalian expression of Human TRIM28

    Depositor

    Michelle Barton

    Article

    Li et al Oncogene. 2017 May 25;36(21):2991-3001.

    Insert

    hTRIM28 (TRIM28 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-MS2-HA-HsRoquin1_AE

    Plasmid

    #148611

    Purpose

    Mammalian Expression of HsRoquin1

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    HsRoquin1 (RC3H1 Human)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. SNAP-Rab5

    Plasmid

    #169069

    Purpose

    Express human Rab5b in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

    Insert

    Rab5B (RAB5B Human)
    

    Tags

    SNAP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJYScr

    Plasmid

    #132719

    Purpose

    Plasmid for an easy-to-construct crRNA delivery vector using hybridized oligonucleotides. Constitutive transcription of crRNA, Spectinomycin resistance

    Depositor

    Jan Marienhagen

    Article

    Krumbach et al ACS Synth Biol. 2019 Dec 11. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT7-7 asyn E46K

    Plasmid

    #105728

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Article

    Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

    Insert

    alpha-synuclein E46K (SNCA Human)
    

    Expression

    Bacterial

    Mutation

    E46K

    Promoter

    T7

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pet21_TurboID_18A

    Plasmid

    #170922

    Purpose

    Express TurboID_18A in Bl21

    Depositor

    Huan Bao

    Article

    Bao et al Commun Biol. 2021 Apr 30;4(1):514. doi:

    Insert

    TurboID-18a
    

    Expression

    Bacterial

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-monSTIM1

    Plasmid

    #161795

    Purpose

    Mammalian expression plasmid of monSTIM1 (ultra-photosensitive optogenetically-activated STIM1 protein)

    Depositor

    Won Do Heo

    Article

    Kim et al Nat Commun. 2020 Jan 10;11(1):210. doi:

    Insert

    MonSTIM1 (STIM1 Human, Mustard Weed)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. AICSDP-49: MYL2-mEGFP

    Plasmid

    #114414

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human MYL2, via Cas9-excisable CAGGS-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

    Insert

    MYL2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTRE3G_SS-HA-V5-LaccID-CD4 TM_Puro

    Plasmid

    #234047

    Purpose

    expresses LaccID on the mammalian cell surface, dox-inducible lentiviral vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Chem Biol. 2025 Aug 1. doi: 10.1038/

    Insert

    LaccID-CD4TM
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDisplay-GRAB-rDA3m-IRES-EGFP-CAAX

    Plasmid

    #208696

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m and a membrane-localized EGFP in mammalian cells

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_rDA3m
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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