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pET42b-BE3

Plasmid

#87437

Purpose

Expresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

Depositor

David Liu

Article

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

BE3 (Apobec1 Bacillus phage PBS2, Rat, Human)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3_pLyn_EGFP

Plasmid

#214605

Purpose

Expresses fluorescent protein EGFP targeted to plasma membrane

Depositor

Peter Dedecker

Article

Valenta et al Talanta. 2024 Mar

Insert

lyn-EGFP

Tags

lyn targetting

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pBABE YAP1

Plasmid

#15682

Depositor

Joan Brugge

Article

Overholtzer et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

Insert

YAP (YAP1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Aug. 31, 2007

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE1

Plasmid

#90335

Purpose

NFkB - p50/p65 pathway gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

NFKB

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pHDR-FOXL2-T2A-tdTomato-PuroTK

Plasmid

#192892

Purpose

HDR donor for inserting FOXL2-T2A-tdTomato reporter

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

FOXL2-tdTomato (FOXL2 Human)

Use

CRISPR

Mutation

T2A-tdTomato fluorescent reporter

Promoter

PGK

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSFFV_sfCherry3C(1-10)

Plasmid

#117482

Purpose

Lentiviral vector for constitutive expression of sfCherry3C(1-10)

Depositor

Bo Huang

Article

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Insert

sfCherry3C(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

5 substitutions (K45R, G52D, T106A, K182R, N194D)…

Promoter

SFFV

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4 [luc2P/STAT4-RE/Hygro] Vector

Plasmid

#236820

Purpose

Express STAT4 response element in luciferase reporter assay

Depositor

Promega Corporation

Has Service

DNA

Insert

STAT4 (STAT4 Human)

Use

Luciferase

Mutation

None

Promoter

minP

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFPC1-hVAP-A KD/MD

Plasmid

#104449

Purpose

Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein A (VAPA Human)

Tags

EGFP

Expression

Mammalian

Mutation

changed K94 to D and M96 to D

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pJSK664

Plasmid

#205130

Purpose

Expression of GFP-2xPX (from SnxA) in mammalian cells

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

tandem 2xPX domain of SnxA

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTW335c3

Plasmid

#237322

Purpose

expresses A. thaliana RbcL RbcS-6xHis

Depositor

Tina Wang

Article

Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

Inserts

A. thaliana RbcL (rbcL Mustard Weed)
A. thaliana RbcS-6xHis

Tags

6xHis and None

Expression

Bacterial

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pSBtet-RH

Plasmid

#60500

Purpose

SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and hygromycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

TCE & RPBSA

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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FRP793_insul-(lexA-box)4-PminCYC1-Citrine-TCYC1

Plasmid

#58434

Purpose

Induces Citrine expression from a promoter containing 4 lexA boxes in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

CitrineA206K

Expression

Yeast

Promoter

insul-(lexA-box)4-PminCYC1

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pDisplay-AP-CFP-TM

Plasmid

#20861

Depositor

Alice Ting

Article

Howarth et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

AP-CFP-TM

Tags

CFP, HA, and myc

Expression

Mammalian

Available Since

April 21, 2009

Availability

Academic Institutions and Nonprofits only

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pTDpelB-CTwinStrep

Plasmid

#45941

Purpose

use to create N-terminal PelB and C-terminal Twin-Strep-tagged fusion proteins for periplasmic translocation via PelB signal sequence

Depositor

Thorben Dammeyer

Article

Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

Type

Empty backbone

Tags

PelB and Twin-Strep

Expression

Bacterial

Promoter

Trc (lactose/IPTG inducible)

Available Since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-PtenWT-Puro

Plasmid

#135676

Purpose

Constitutive expression (cMV promoter) of wildtype Pten cDNA, Puromycin selection

Depositor

Florian Karreth

Insert

Pten (Pten Mouse)

Use

Lentiviral

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgPenk

Plasmid

#159916

Purpose

Mutagenesis of Penk

Depositor

Larry Zweifel

Insert

Penk (Penk Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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mCh-Mfn2

Plasmid

#141156

Purpose

Mammalian expression and labeling mitofusin 2

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

Mitofusin 2 (MFN2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W

Plasmid

#67974

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/BFP markers

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2ABFP cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PM-GA

Plasmid

#209877

Purpose

Dimerization dependent fluorescent protein GA anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddGFP A

Tags

Targeting domain of Gap43 MLCCMRRTKQVEKNDDQKI

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pROF021

Plasmid

#155326

Purpose

Expression of PULSE-controlled FLuc

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

(etr)8-(C120)5-PhCMVmin-FLuc-TSV40

Use

Luciferase and Synthetic Biology; Eukaryotic expr…

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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