176,063 results

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HBT-pcoCas9

Plasmid

#52254

Purpose

transient expression of pcoCas9 gene in plant cells

Depositor

Jen Sheen

Publication

Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

Insert

pcoCas9

Use

CRISPR; Plant expression

Tags

FLAG and NLS

Promoter

hybrid constitutive promoter 35SPPDK

Available since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pmTurquoise2-NES

Plasmid

#36206

Purpose

In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

NES

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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CLuc-FKBP

Plasmid

#31184

Depositor

David Piwnica-Worms

Publication

Luker et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

Insert

C-terminus of Firefly Luciferase and FK506-binding protein 12 (FKBP1A Human)

Expression

Mammalian

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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EGFP-Rab5A S34N

Plasmid

#28045

Depositor

Qing Zhong

Publication

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rab5A (RAB5A Human)

Tags

EGFP on N-term

Expression

Mammalian

Mutation

S34N

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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Vinculin-venus

Plasmid

#27300

Depositor

Martin Schwartz

Publication

Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

Insert

Vinculin (VCL Chicken)

Tags

Venus A206K

Expression

Mammalian

Promoter

CMV

Available since

Jan. 3, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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Flag-HA-USP5

Plasmid

#22590

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP5 (USP5 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pWZL Neo Myr Flag PKM2

Plasmid

#20585

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PKM2 (PKM Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3.1/hChR2-EYFP

Plasmid

#20939

Purpose

Mammalian expression of humanized ChR2 fused to EYFP

Depositor

Karl Deisseroth

Publication

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Expression

Mammalian

Promoter

CMV

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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HA-HIF2alpha-P405A/P531A-pBabe-puro

Plasmid

#19006

Depositor

William Kaelin

Publication

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: P405A and P531A

Available since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pAd-Track Flag-SIRT1

Plasmid

#8438

Depositor

Pere Puigserver

Publication

Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

Insert

SIRT1 (Sirt1 Mouse)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Available since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pRK5 alpha6

Plasmid

#16036

Depositor

Filippo Giancotti

Publication

Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

Insert

alpha6 (ITGA6 Human)

Expression

Mammalian

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3-TLR1-YFP

Plasmid

#13014

Depositor

Doug Golenbock

Insert

Toll-Like Receptor 1 (TLR1 Human)

Tags

YFP

Expression

Mammalian

Available since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pDY0663 ACTB atgRNA with v2 scaffold

Plasmid

#179108

Purpose

ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffold

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

ACTB N-term PBS 13 RT 29 AttB 46 atgRNA with v2 scaffold

Use

CRISPR

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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ABE8.20-m

Plasmid

#136300

Purpose

expresses ABE8.20-m in mammalian cells

Depositor

Nicole Gaudelli

Publication

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.20-m

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pORTMAGE-Ec1

Plasmid

#138474

Purpose

Enables high-efficiency recombineering in E. coli

Depositor

George Church

Publication

Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

Inserts

CspRecT
mutL E32K

Expression

Bacterial

Mutation

E32K

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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CROPseq-puro-v2

Plasmid

#127458

Purpose

Entry plasmid for guide cloning

Depositor

Paul Blainey

Publication

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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LRG2.1

Plasmid

#108098

Purpose

Lentiviral expression plasmid of sgRNA with GFP

Depositor

Christopher Vakoc

Publication

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for sgRNA expression and EFS promoter…

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

51 Articles

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pTP1005

Plasmid

#104160

Purpose

Bacterial expression plasmid of anti-mouse IgG2a Fc nanobody TP1129 (3x Cysteines)

Depositor

Dirk Görlich

Publication

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse IgG2a Fc specific nanobody TP1129 (3xCysteines)

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-TRE-EGFP

Plasmid

#89875

Purpose

EGFP reporter vector of iTango system for AAV production

Depositor

Hyungbae Kwon

Publication

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

tetO-EGFP

Use

AAV

Expression

Mammalian

Promoter

tetO

Available since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2

Plasmid

#74009

Purpose

Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter

Depositor

Filippo Del Bene

Publication

Di Donato et al Genome Res. 2016 Mar 8.

Inserts

5x UAS
Cas9
T2A
GFP
U6 promoter

Use

CRISPR

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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