172,934 results

Showing: 2921 - 2940 of 172934 results

Results per page:

Sort results by:

pTRIP-CMV-GFP-FLAG-cGAS E225A-D227A

Plasmid

#86674

Purpose

Expresses catalytically-inactive GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

Depositor

Nicolas Manel

Article

Raab et al Science. 2016 Apr 15;352(6283):359-62. d

Insert

GFP-FLAG-cGAS E225A/D227A

Use

Lentiviral

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRL055 pDESTattB_cryaa:Venus

Plasmid

#213509

Purpose

attB-containing pDEST plasmid, for use in Multisite Gateway reaction

Depositor

Christian Mosimann

Article

Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

Type

Empty backbone

Use

Zebrafish expression

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-HaloKbp1b

Plasmid

#228794

Purpose

Chemigenetic indicator for potassium ion - low affinity

Depositor

Robert Campbell

Article

Cheng et al J Am Chem Soc. 2024 Dec 25;146(51):35117

Insert

HaloKbp1b

Tags

His-Tag

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
lenti-pegRNA-puro backbone

Plasmid

#214085

Purpose

Lentiviral backbone plasmid to insert pegRNAs of interest. The cloning site is BsmbI.

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

hU6, EF1a

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-MMLVgag-3xNES-Cas9

Plasmid

#181752

Purpose

MMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.

Depositor

David Liu

Article

Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

Insert

MMLV-Gag-3xNES-Cas9 (Spy)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mApple-Alpha-V-Integrin-25

Plasmid

#54866

Purpose

Localization: Focal Adhesions, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Insert

Alpha-V Integrin (ITGAV Human)

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pET16B.Pfu

Plasmid

#12509

Purpose

Bacterial expression of Pfu DNA polymerase

Depositor

Sung-Hou Kim

Article

Goldman et al Acta Crystallogr D Biol Crystallogr. 199

Insert

Pfu DNA polymerase

Expression

Bacterial

Mutation

Y218H, Q462R, F533L, K559I and V683A (mutations w…

Available Since

Sept. 18, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3b-8XGliBS:EGFP

Plasmid

#84602

Purpose

Gli protein-dependent EGFP reporter for analysis of Hedgehog signaling

Depositor

James Chen

Article

Hyman et al Proc Natl Acad Sci U S A. 2009 Aug 18;10

Insert

eGFP

Expression

Mammalian

Promoter

8X Gli-binding site

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
TETv4

Plasmid

#167983

Purpose

Expresses TETv4 (TET1CD-XTEN80-dCas9-3xNLS-P2A-BFP) downstream of the CAG promoter

Depositor

Luke Gilbert

Article

Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

Insert

TETv4 (TET1CD-XTEN80-dCas9)

Use

CRISPR and Synthetic Biology

Tags

3xNLS, TET1 catalytic domain, and tagBFP

Expression

Mammalian

Promoter

CAG

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP

Plasmid

#60903

Purpose

Expressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activation

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

Cas9 dead

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
AMPK-SPARK

Plasmid

#226255

Purpose

phases-of-separation based activity reporter of AMPK

Depositor

Roland Malli

Article

Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

Insert

AMPK-SPARK

Expression

Mammalian

Available Since

Oct. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pSM41

Plasmid

#235728

Purpose

Protein expression of FL His6- human VPS34 in bacteria

Depositor

Roger Williams

Article

Ohashi et al Elife. 2020 Jun 30;9:e58281. doi: 10.755

Insert

VPS34 (PIK3C3 Human)

Tags

6xHis-TEV

Expression

Bacterial

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pREDTAI

Plasmid

#51627

Purpose

E coli Genetic Modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

I-SceI (SCEI Budding Yeast)

Use

Red recombinase and i-scei endonuclease

Expression

Bacterial

Promoter

trc

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti PGK Blast V5-LUC (w528-1)

Plasmid

#19166

Purpose

3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase

Use

Lentiviral and Luciferase

Tags

V5

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pRRL-MHCK7-GCaMP6

Plasmid

#65042

Purpose

Lentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7

Depositor

Nenad Bursac

Article

Madden et al eLife. 2015 Jan 9;4:e04885. doi: 10.7554

Insert

GCaMP6

Use

Lentiviral

Tags

6xHis, T7 tag, and Xpress epitope

Expression

Mammalian

Promoter

MHCK7

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pTT5-hSDC1

Plasmid

#52326

Purpose

expresses human syndecan 1 in HEK293-EBNA1 (293E) suspension culture.

Depositor

Gordon Laurie

Article

Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

Insert

SDC1 (SDC1 Human)

Expression

Mammalian

Mutation

R117Q mutation (R95Q according to numbering in Fi…

Promoter

CMV

Available Since

Dec. 8, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRX-IPU-GFP-MYH9

Plasmid

#168273

Purpose

Expresses MYH9 in mammalian cells

Depositor

Noboru Mizushima

Article

Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

Insert

MYH9 (MYH9 Human)

Tags

FLAG

Expression

Mammalian

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX_HA

Plasmid

#178534

Purpose

Modifies pLX_317 backbone to express c-term HA tag (instead of c-term V5 tag)

Depositor

William Hahn

Article

Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

Type

Empty backbone

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIV-Zsgreen

Plasmid

#18121

Depositor

Bryan Welm, Zena Werb

Article

Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102

Insert

Zsgreen

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 10, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pBA904

Plasmid

#122238

Purpose

Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.

Depositor

Jonathan Weissman

Article

Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

Insert

sgRNA with cs1 in stem loop 2 of the constant region

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge