pSKDuet01
(Plasmid #12172)

• Depositing Lab: Hideo Iwai
• Publication: Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

Backbone

• Vector backbone: pRSFDuet
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 3757
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NpuDnaE
• Alt name: DnaE N-intein
• Alt name: intein
• Species: Nostoc punctiforme
• Insert Size (bp): 306
• Tag / Fusion Protein:
  • His-GB1 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: Duet-MCS1-fw (5-GGATCTCGACGCTCTCCCT)

Resource Information

• Articles Citing this Plasmid:
  • 19 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pSKDuet01 contains two NdeI sites in the plasmid. To clone a gene of
interest between NdeI and BamHI for constructing a fusion protein with
NpuDnaE N-intein, please use pHYRSF1 (http://www.addgene.org/34549/), which lacks the second T7 promoter and NdeI site.