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pSKDuet01
(Plasmid #12172)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12172 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRSFDuet
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3757
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NpuDnaE
  • Alt name
    DnaE N-intein
  • Alt name
    intein
  • Species
    Nostoc punctiforme
  • Insert Size (bp)
    306
  • Tag / Fusion Protein
    • His-GB1 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer Duet-MCS1-fw (5-GGATCTCGACGCTCTCCCT)
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

pSKDuet01 contains two NdeI sites in the plasmid. To clone a gene of
interest between NdeI and BamHI for constructing a fusion protein with
NpuDnaE N-intein, please use pHYRSF1 (http://www.addgene.org/34549/), which lacks the second T7 promoter and NdeI site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSKDuet01 was a gift from Hideo Iwai (Addgene plasmid # 12172 ; http://n2t.net/addgene:12172 ; RRID:Addgene_12172)
  • For your References section:

    Highly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. Iwai H, Zuger S, Jin J, Tam PH. FEBS Lett. 2006 Mar 20. 580(7):1853-8. 10.1016/j.febslet.2006.02.045 PubMed 16516207