|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12172||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3757
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameDnaE N-intein
Insert Size (bp)306
/ Fusion Protein
- His-GB1 (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer Duet-MCS1-fw (5-GGATCTCGACGCTCTCCCT) (Common Sequencing Primers)
pSKDuet01 contains two NdeI sites in the plasmid. To clone a gene of
interest between NdeI and BamHI for constructing a fusion protein with
NpuDnaE N-intein, please use pHYRSF1 (http://www.addgene.org/34549/), which lacks the second T7 promoter and NdeI site.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSKDuet01 was a gift from Hideo Iwai (Addgene plasmid # 12172 ; http://n2t.net/addgene:12172 ; RRID:Addgene_12172)
For your References section:Highly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. Iwai H, Zuger S, Jin J, Tam PH. FEBS Lett. 2006 Mar 20. 580(7):1853-8. 10.1016/j.febslet.2006.02.045 PubMed 16516207