Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


pSKDuet01 Citations (19)

Originally described in: Highly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme.
Iwai H, Zuger S, Jin J, Tam PH FEBS Lett. 2006 Mar 20. 580(7):1853-8.
PubMed Journal

Articles Citing pSKDuet01

Segmental isotopic labeling of multi-domain and fusion proteins by protein trans-splicing in vivo and in vitro. Muona M, Aranko AS, Raulinaitis V, Iwaï H. Nat Protoc. 2010 . 5(3):574-87. PubMed

Associated Plasmids

Split-intein mediated re-assembly of genetically encoded Ca(2+) indicators. Wong SS, Kotera I, Mills E, Suzuki H, Truong K. Cell Calcium. 2011 Nov 29. PubMed

Associated Plasmids

Simultaneous assembly of two target proteins using split inteins for live cell imaging. Wong S, Mills E, Truong K. Protein Eng Des Sel. 2013 Mar;26(3):207-13. doi: 10.1093/protein/gzs100. Epub 2012 Dec 4. PubMed
Parts-based assembly of synthetic transmembrane proteins in mammalian cells. Nagaraj S, Wong S, Truong K. ACS Synth Biol. 2012 Apr 20;1(4):111-7. doi: 10.1021/sb200007r. Epub 2011 Nov 11. PubMed
A general purification platform for toxic proteins based on intein trans-splicing. Shi C, Tarimala A, Meng Q, Wood DW. Appl Microbiol Biotechnol. 2014 Nov;98(22):9425-35. doi: 10.1007/s00253-014-6080-1. Epub 2014 Oct 9. PubMed
A dual-intein autoprocessing domain that directs synchronized protein co-expression in both prokaryotes and eukaryotes. Zhang B, Rapolu M, Liang Z, Han Z, Williams PG, Su WW. Sci Rep. 2015 Feb 25;5:8541. doi: 10.1038/srep08541. PubMed
An Engineered Split Intein for Photoactivated Protein Trans-Splicing. Wong S, Mosabbir AA, Truong K. PLoS One. 2015 Aug 28;10(8):e0135965. doi: 10.1371/journal.pone.0135965. eCollection 2015. PubMed
Locked and proteolysis-based transcription activator-like effector (TALE) regulation. Lonzaric J, Lebar T, Majerle A, Mancek-Keber M, Jerala R. Nucleic Acids Res. 2016 Jan 8. pii: gkv1541. PubMed
Generation of camelid VHH bispecific constructs via in-cell intein-mediated protein trans-splicing. Shibuya Y, Haga N, Asano R, Nakazawa H, Hattori T, Takeda D, Sugiyama A, Kurotani R, Kumagai I, Umetsu M, Makabe K. Protein Eng Des Sel. 2017 Jan;30(1):15-21. Epub 2016 Nov 23. PubMed
Intein-mediated assembly of tunable scaffoldins for facile synthesis of designer cellulosomes. Han Z, Su WW. Appl Microbiol Biotechnol. 2018 Feb;102(3):1331-1342. doi: 10.1007/s00253-017-8701-y. Epub 2017 Dec 23. PubMed
Intein-mediated backbone cyclization of entolimod confers enhanced radioprotective activity in mouse models. Ye B, Shen W, Shi M, Zhang Y, Xu C, Zhao Z. PeerJ. 2018 Jun 18;6:e5043. doi: 10.7717/peerj.5043. eCollection 2018. PubMed
Design of fast proteolysis-based signaling and logic circuits in mammalian cells. Fink T, Lonzaric J, Praznik A, Plaper T, Merljak E, Leben K, Jerala N, Lebar T, Strmsek Z, Lapenta F, Bencina M, Jerala R. Nat Chem Biol. 2019 Feb;15(2):115-122. doi: 10.1038/s41589-018-0181-6. Epub 2018 Dec 10. PubMed

Associated Plasmids

Intein-ubiquitin chimeric domain for coordinated protein coexpression. Zhang B, Han Z, Kumar S, Gupta M, Su WW. J Biotechnol. 2019 Oct 10;304:38-43. doi: 10.1016/j.jbiotec.2019.08.006. Epub 2019 Aug 12. PubMed
Smad7 Binds Differently to Individual and Tandem WW3 and WW4 Domains of WWP2 Ubiquitin Ligase Isoforms. Wahl LC, Watt JE, Yim HTT, De Bourcier D, Tolchard J, Soond SM, Blumenschein TMA, Chantry A. Int J Mol Sci. 2019 Sep 21;20(19). pii: ijms20194682. doi: 10.3390/ijms20194682. PubMed
The crystal structure of the naturally split gp41-1 intein guides the engineering of orthogonal split inteins from cis-splicing inteins. Beyer HM, Mikula KM, Li M, Wlodawer A, Iwai H. FEBS J. 2019 Oct 30. doi: 10.1111/febs.15113. PubMed

Associated Plasmids

Quantification, regulation and production of 5-aminolevulinic acid by green fluorescent protein in recombinant Escherichia coli. Tan SI, You SC, Shih IT, Ng IS. J Biosci Bioeng. 2019 Oct 30. pii: S1389-1723(19)30846-1. doi: 10.1016/j.jbiosc.2019.10.005. PubMed
Metabolic enzyme clustering by coiled coils improves the biosynthesis of resveratrol and mevalonate. Fink T, Stevovic B, Verwaal R, Roubos JA, Gaber R, Bencina M, Jerala R, Gradisar H. AMB Express. 2020 May 24;10(1):97. doi: 10.1186/s13568-020-01031-5. PubMed

Associated Plasmids

Dengue virus protease activity modulated by dynamics of protease cofactor. Lee WHK, Liu W, Fan JS, Yang D. Biophys J. 2021 Jun 15;120(12):2444-2453. doi: 10.1016/j.bpj.2021.04.015. Epub 2021 Apr 22. PubMed
The NMR structure of the engineered halophilic DnaE intein for segmental isotopic labeling using conditional protein splicing. Heikkinen HA, Aranko AS, Iwai H. J Magn Reson. 2022 May;338:107195. doi: 10.1016/j.jmr.2022.107195. Epub 2022 Mar 18. PubMed

If you have published an article using this material, please email us at [email protected] to have your article added to this page.