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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 39319 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepmCherry-C1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry-3xNLS
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SpeciesSynthetic
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Insert Size (bp)798
- Promoter CMV
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Tag
/ Fusion Protein
- 3xSV40LT NLS
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byRoger Y. Tsien, mCherry
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmCherry-NLS was a gift from Martin Offterdinger (Addgene plasmid # 39319 ; http://n2t.net/addgene:39319 ; RRID:Addgene_39319) -
For your References section:
Analysis of the cellular uptake and nuclear delivery of insulin-like growth factor binding protein-3 in human osteosarcoma cells. Micutkova L, Hermann M, Offterdinger M, Hess MW, Matscheski A, Pircher H, Muck C, Ebner HL, Laich A, Ferrando-May E, Zwerschke W, Huber LA, Jansen-Durr P. Int J Cancer. 2012 Apr 1;130(7):1544-57. doi: 10.1002/ijc.26149. Epub 2011 Aug 2. 10.1002/ijc.26149 PubMed 21520041
Map uploaded by the depositor.
