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Apple-53BP1trunc
(Plasmid #69531)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 69531 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLVX
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 8003
  • Total vector size (bp) 10184
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    53BP1
  • Alt name
    TP53BP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1473
  • Entrez Gene
    TP53BP1 (a.k.a. 53BP1, TDRD30, p202, p53BP1)
  • Promoter CMV
  • Tag / Fusion Protein
    • Apple fluorescent protein (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrGI (not destroyed)
  • 3′ cloning site XbaI (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer custom
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Note: This construct contains amino acids 1220-1709 of 53BP1 followed by the short peptide VNDLDNSTG-STOP. This additional peptide does not affect reporter activity, and the plasmid functions as described in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Apple-53BP1trunc was a gift from Ralph Weissleder (Addgene plasmid # 69531 ; http://n2t.net/addgene:69531 ; RRID:Addgene_69531)
  • For your References section:

    Single cell resolution in vivo imaging of DNA damage following PARP inhibition. Yang KS, Kohler RH, Landon M, Giedt R, Weissleder R. Sci Rep. 2015 May 18;5:10129. doi: 10.1038/srep10129. 10.1038/srep10129 PubMed 25984718