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PurposeDNA damage inducible Red Fluorescent Protein for mammalian expression
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Depositing Lab
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Depositing OrganizationMassachusetts Institute of Technology
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 52432 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 52432-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 52432-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepTurboRFP-PRL
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Backbone manufacturerEvrogen
- Backbone size w/o insert (bp) 4127
- Total vector size (bp) 4794
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep21-p53 Tandem Response Element Promoter
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SpeciesM. musculus (mouse)
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Insert Size (bp)667
- Promoter 13X repeated p21-p53 response element
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Tag
/ Fusion Protein
- TurboRFP (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer SV40pro_F_primer
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byVector backbone was purchased from Evrogen and the insert was cloned by Blue Heron.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 52432-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 52432-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p21-Reporter was a gift from Joel Voldman (Addgene plasmid # 52432 ; http://n2t.net/addgene:52432 ; RRID:Addgene_52432) -
For your References section:
Cell-based biosensor to report DNA damage in micro- and nanosystems. Fendyur A, Varma S, Lo CT, Voldman J. Anal Chem. 2014 Aug 5;86(15):7598-605. doi: 10.1021/ac501412c. Epub 2014 Jul 7. 10.1021/ac501412c PubMed 25001406
Map uploaded by the depositor.
