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pUC19-HRP-C
(Plasmid #40163)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 40163 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2700
  • Total vector size (bp) 3700
  • Vector type
    coli cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Horseradish peroxidase
  • Alt name
    HRP
  • Species
    Synthetic; horseradish
  • Insert Size (bp)
    966
  • Tag / Fusion Protein
    • Six histidine (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer M13 forward
  • 3′ sequencing primer M13 reverse
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Synthetic gene with codons optimized for mammalian expression. See original reference: Hartmann, C, and Ortiz de Montellano, P, "Baculovirus expression and characterization of catalytically active horseradish peroxidase" (1992) Arch Biochem Biophys, 297, 61-72.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19-HRP-C was a gift from Paul Ortiz de Montellano (Addgene plasmid # 40163 ; http://n2t.net/addgene:40163 ; RRID:Addgene_40163)
  • For your References section:

    Baculovirus expression and characterization of catalytically active horseradish peroxidase. Hartmann C, Ortiz de Montellano PR. Arch Biochem Biophys. 1992 Aug 15;297(1):61-72. 10.1016/0003-9861(92)90641-9 PubMed 1637184