163,606 results

1. pU6a:sgRNA#1

   Plasmid

   #64245

   Purpose

   expresses sgRNA under U6a promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. peGFP-N1-Cofilin

   Plasmid

   #186747

   Purpose

   Expresses wildtype GFP-tagged human cofilin in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

   Insert

   Cofilin-1 (CFL1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-Lamin-A

   Plasmid

   #206027

   Purpose

   Expresses GFP-tagged Lamin A in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

   Insert

   Lamin-A (LMNA Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCFD5

   Plasmid

   #73914

   Purpose

   multiplex gRNA plasmid

   Depositor

   Simon Bullock

   Article

   Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

   Insert

   dU6-3:tRNA:gRNA:tRNA:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   dU6:3

   Available Since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-28a_6H-MMLV_RT_D524N-6H

   Plasmid

   #166945

   Purpose

   Bacterial expression of M-MLV reverse transcriptase

   Depositor

   Robert Tjian

   Article

   Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

   Insert

   gag-pol
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   D524N, H8Y

   Promoter

   T7

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBMN(CMV-copGFP-Puro-SMAR)

   Plasmid

   #80391

   Purpose

   expression copGFP and Puromycine, with an S/MAR element for episomal maintaince

   Depositor

   Magnus Essand

   Article

   Jin et al EMBO Mol Med. 2016 May 6. pii: e20150586

   Insert

   copGFP-T2A-Puromycine
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV Puro

   Plasmid

   #68469

   Purpose

   Retroviral plasmid with multiple cloning site and puromycin resistance

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Puromycin Resistance
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pGK

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVE-eGFP

   Plasmid

   #52581

   Purpose

   Lentiviral transfer vector driving expression of eGFP in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Brunger et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha

   Available Since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pICE-HA-NLS-I-PpoI

   Plasmid

   #46963

   Purpose

   For constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Insert

   NLS-I-PpoI
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TET

   Available Since

   Aug. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. LifeAct-mNeonGreen

    Plasmid

    #98877

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    LifeAct
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSIE1

    Plasmid

    #136355

    Purpose

    Selection by Inducible Effectors

    Depositor

    Andrew Goodman

    Article

    Bencivenga-Barry et al J Bacteriol. 2020 Jan 15;202(3). pii: JB

    Inserts

    Type VI effector bfe1
    Type VI effector bte1
    BT_4676
    tetR
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    ATG removed to add signal sequence before gene

    Promoter

    P1TDP-GHO23

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMOS008: GCaMP6F calcium sensor (cytosolic)

    Plasmid

    #163045

    Purpose

    GCaMP6F calcium sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    GCaMP6F
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-FLAG-SF2

    Plasmid

    #99021

    Purpose

    Mammalian expression of SF2

    Depositor

    Honglin Chen

    Article

    Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

    Insert

    SF2 (SRSF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE2-EF1a-GFP1-10-IRES-Puro

    Plasmid

    #211467

    Purpose

    Lentivirus backbone expressing GFP1-10

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    GFP1-10
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3-HA-human MYCN

    Plasmid

    #74163

    Purpose

    Mammalian expression of human MycN

    Depositor

    Martine Roussel

    Article

    Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

    Insert

    MycN (MYCN Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGP-AAV-syn-jGCaMP8s-WPRE

    Plasmid

    #162374

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8s
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S26M F286Y Q315H

    Promoter

    Synapsin

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPSU150

    Plasmid

    #173836

    Purpose

    Expresses 150 kD Penn State ladder protein

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    STRHSTPABMBPpepN
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEMS-II

    Plasmid

    #204765

    Purpose

    GEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577)

    Depositor

    Kate Meyer

    Article

    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    EGFP-m6Alinker-DHFR
    

    Use

    Tags

    Expression

    Mutation

    mutated all RACs in EGFP to RGC/RCC

    Promoter

    CMV

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.Syn.NES.jRCaMP1a.WPRE.SV40

    Plasmid

    #100848

    Purpose

    AAV expression of jRCaMP1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV1 and AAV9

    Insert

    jRCaMP1a
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLentiCas9-T2A-GFP

    Plasmid

    #78548

    Purpose

    Cas9 protein with GFP

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    T2A-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    In frame with Cas9

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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