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RFP-zyxin
(Plasmid #26720)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26720 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDsRed-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Zyxin
  • Species
    H. sapiens (human); unknown
  • Tag / Fusion Protein
    • RFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RFP-zyxin was a gift from Anna Huttenlocher (Addgene plasmid # 26720 ; http://n2t.net/addgene:26720 ; RRID:Addgene_26720)
  • For your References section:

    Regulation of focal complex composition and disassembly by the calcium-dependent protease calpain. Bhatt A, Kaverina I, Otey C, Huttenlocher A. J Cell Sci. 2002 Sep 1. 115(Pt 17):3415-25. PubMed 12154072