163,606 results

1. dCas9-KRAB

   Plasmid

   #110820

   Purpose

   Conventional dCas9 repressor-dCas9-KRAB

   Depositor

   Alejandro Chavez, George Church

   Article

   Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

   Insert

   dCas9-KRAB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CMV

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. gRNA_Cloning Vector

   Plasmid

   #41824

   Purpose

   An empty gRNA expression vector, used to create a gRNA to a specific sequence.

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   AflII site for Gibson Assembly

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG426GPD-ccdB

   Plasmid

   #14156

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. TK4-NLS-EGFP

   Plasmid

   #239046

   Purpose

   PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation

   Depositor

   Marius Wernig

   Inserts

   EGFP
   puroR-T2A-mycNLS-mTagBFP2
   

   Use

   Tags

   T2A-mycNLS-mTagBFP2 and c-myc-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and EF1a

   Available Since

   May 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9(BB)-2A-Neo

   Plasmid

   #127762

   Purpose

   Used for expression of sgRNA in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCSII-EF/mt-(n1)StayGold

   Plasmid

   #185823

   Purpose

   A soluble photostable fluorescent marker of the mitochondrial matrix. The StayGold gene has codon optimization for mammalian expression.

   Depositor

   Atsushi Miyawaki

   Article

   Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

   Insert

   mt-(n1)StayGold
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRG2

   Plasmid

   #104174

   Purpose

   sgRNA expression

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-AID-PcrA M6-UGI-T2A-mTagBFP2

   Plasmid

   #221225

   Purpose

   Express HACE Editor as a fusion of AID cytidine deaminase and an optimized PcrA M6 helicase with mTagBFP2 reporter

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   AID-PcrA M6-UGI-T2A-mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-3'3xFlag

   Plasmid

   #208616

   Purpose

   Empty vector for mammalian expression, insert 3xFlag tag using BamHI andAflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with 3xFlag

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pwtCas9-bacteria

    Plasmid

    #44250

    Purpose

    aTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    wild-type Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO-1

    Available Since

    April 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti6.2-ccdB-3xFLAG-V5

    Plasmid

    #87071

    Purpose

    Lentiviral Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tag

    Depositor

    Susan Lindquist, Mikko Taipale

    Article

    Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3xFLAG-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBa-LSS-GFP-LDLR wt

    Plasmid

    #98184

    Purpose

    Low Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.

    Depositor

    Marvin Bentley, Gary Banker

    Article

    Jenkins et al J Cell Biol. 2012 Aug 20;198(4):749-61.

    Insert

    LDLR (LDLR Human)
    

    Use

    Tags

    GFP and LDLR signal sequence, N-term of GFP so GF…

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Chicken Beta Actin

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST/hCx43-EGFP-N1

    Plasmid

    #40907

    Depositor

    Robin Shaw

    Article

    Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

    Insert

    Connexin 43 (GJA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    No stop codon to create c-terminal fusion

    Promoter

    CMV

    Available Since

    Nov. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. eGFP-SUN1

    Plasmid

    #213515

    Purpose

    Expresses human eGFP-SUN1 in mammalian cells

    Depositor

    Karim Mekhail

    Article

    Shokrollahi et al bioRxiv 2023.05.07.539750

    Insert

    SUN1 (SUN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SUN1 has a silent mutation on its gene (nucleotid…

    Promoter

    CMV

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-synapsin-HaloCaMP1a-EGFP

    Plasmid

    #138327

    Purpose

    chemigenetic calcium indicator

    Depositor

    Eric Schreiter

    Article

    Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

    Has Service

    AAV1 and AAV9

    Insert

    HaloCaMP1a
    

    Use

    AAV

    Tags

    EGFP, His-tag, and Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available Since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. HyPer7.2DAAO-NLS

    Plasmid

    #168302

    Purpose

    Chemogenetic fluorescent H2O2 reporter targeted to the nucleus

    Depositor

    Thomas Michel

    Article

    Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

    Insert

    HyPer7.2DAAO-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-HA-PD-L1

    Plasmid

    #121492

    Purpose

    Expression of human PD-L1 WT in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    PD-L1 (CD274 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUltra

    Plasmid

    #24129

    Purpose

    3rd generation Lentiviral vector for bi-cistronic expression of EGFP and the gene of interest

    Depositor

    Malcolm Moore

    Article

    Lou et al PLoS One. 2012;7(3):e33093. doi: 10.1371

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-J chain

    Plasmid

    #145146

    Purpose

    Mammalian expression plasmid for J chain of polymeric antibody classes

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    immunoglobulin J chain (JCHAIN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAVS1-MAFB-IRF8-CEBPA

    Plasmid

    #190720

    Purpose

    For microglia differentiation from iPSC

    Depositor

    Li Gan

    Article

    Drager et al Nat Neurosci. 2022 Aug 11. pii: 10.1038/

    Insert

    MAFB-IRF8-CEBPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge