|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16583||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7700
Vector typeMammalian Expression, Adenoviral
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namecre recombinase
Insert Size (bp)1100
Entrez Genecre (a.k.a. P1_gp003)
/ Fusion Protein
- HA (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site See map (not destroyed)
- 3′ cloning site See map (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Cre expression vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pShuttle-Cre-HA was a gift from Bert Vogelstein (Addgene plasmid # 16583 ; http://n2t.net/addgene:16583 ; RRID:Addgene_16583)
For your References section:Securin is required for chromosomal stability in human cells. Jallepalli PV, Waizenegger IC, Bunz F, Langer S, Speicher MR, Peters JM, Kinzler KW, Vogelstein B, Lengauer C. Cell. 2001 May 18. 105(4):445-57. 10.1016/S0092-8674(01)00340-3 PubMed 11371342