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Isolating live cell clones from barcoded populations using CRISPRa-inducible reporters.
Umkehrer C, Holstein F, Formenti L, Jude J, Froussios K, Neumann T, Cronin SM, Haas L, Lipp JJ, Burkard TR, Fellner M, Wiesner T, Zuber J, Obenauf AC
Nat Biotechnol. 2020 Jul 27. pii: 10.1038/s41587-020-0614-0. doi: 10.1038/s41587-020-0614-0.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
154193dCas9-VPR_P2A_mCherryLentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry marker
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154194Dual-sgRNA_hU6-mU6Lentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.
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154195CaTCH BC1Lentiviral CaTCH barcoding plasmid harboring a single barcode cassette (BC1)
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154196CaTCH Dual-sgRNA_sgBC1-A_sgBC1-CLentiviral expression plasmid encoding two sgRNAs for targeting of the CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.
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154197CaTCH Dual-sgRNA_sgBC2-A_sgBC2-CLentiviral expression plasmid encoding two sgRNAs without a target. These can be used as a off-target control for CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.
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