We narrowed to 3,394 results for: zebrafish

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458 p3E-2A-EGFP-CAAXpA

Plasmid

#195968

Purpose

Gateway p3E 2A fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-EGFP-CAAX (membrane)

Use

Other

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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LbCas12a-2C-NLS in pCSDest

Plasmid

#126638

Purpose

Expresses LbCas12a-2C-NLS in mammalian cell

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

LbCas12a-2C-NLS

Use

CRISPR

Tags

3xHuman influenza hemagglutinin (HA)-Tag

Expression

Mammalian

Promoter

CMV IE94 promoter

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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Zed_vector_tol2_+15.2prox1a:EGFP_XCA:DsRed2

Plasmid

#218206

Purpose

Zebrafish +15.2prox1a enhancer reporter in the FCLV from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vector

Depositor

Katarzyna Koltowska

Article

Panara et al Development. 2024 May 1;151(9):dev202525

Insert

+15.2prox1a

Use

Zebrafish expression

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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acta2_mCherry_pA_pDestTolpA2

Plasmid

#78685

Purpose

acta2:mCherry transgenesis construct

Depositor

Sarah Childs

Article

Whitesell et al PLoS One. 2014 Mar 3;9(3):e90590. doi: 1

Insert

acta2 promoter (acta2 Zebrafish)

Use

Unspecified

Tags

mCherry

Promoter

acta2

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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acta2_GFP_pA_pDestTolpA2

Plasmid

#78684

Purpose

acta2:GFP transgenesis construct

Depositor

Sarah Childs

Article

Whitesell et al PLoS One. 2014 Mar 3;9(3):e90590. doi: 1

Insert

acta2 promoter (acta2 Zebrafish)

Use

Unspecified

Promoter

acta2

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mCerulean 156-239-GGGS

Plasmid

#162616

Purpose

Allows for transcription of control mCerulean fluorophore half for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

mCerulean

Expression

Mammalian

Mutation

156-239

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mKate2

Plasmid

#162624

Purpose

Allows for transcription of mKate2 control for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

mKate2

Expression

Mammalian

Promoter

SP6

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCK069 desmaMCS:minprom_mApple,exorh:EGFP

Plasmid

#195982

Purpose

Expression construct

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

desmin a regulatory elements, mouse beta-globin minimal promoter mApple, exorh:EGFP (desma Zebrafish)

Use

Other

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2

Plasmid

#218207

Purpose

Zebrafish -2.1prox1a_1 (mouse conserved) enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vector

Depositor

Katarzyna Koltowska

Article

Panara et al Development. 2024 May 1;151(9):dev202525

Insert

-2.1prox1a_1

Use

Zebrafish expression

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+ mWnt5a

Plasmid

#102860

Purpose

Expresses mouse Wnt5a in mammalian cells, zebrafish or Xenopus

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Mouse Wnt5a

Use

Zebrafish expression; xenopus expression

Expression

Mammalian

Promoter

CMV; SP6

Available Since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_antiCD19_eZ3_Gal4VP64

Plasmid

#169917

Purpose

Expression of a synNotch receptor containing antiCD19, a zebrafish Notch3 core with an additional EGF repeat, and Gal4VP64.

Depositor

Zhili Rong

Article

Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

Insert

antiCD19-Notch3-GL4VP64

Use

Lentiviral

Expression

Mammalian

Mutation

Additional EGF repeat between the extracellular d…

Promoter

PGK

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pSlick-Neo-RECK

Plasmid

#121986

Purpose

Tetracycline-inducible expression of untagged full-length RECK

Depositor

GuangJun Zhang

Article

Kumari et al Oncotarget. 2018 May 4;9(34):23494-23504

Insert

RECK (RECK Human)

Use

Lentiviral

Promoter

Tight TRE promoter

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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AsCas12a-2C-NLS in pCSDest

Plasmid

#126637

Purpose

Expresses AsCas12a-2C-NLS in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

AsCas12a-2C-NLS

Use

CRISPR

Tags

3xHuman influenza hemagglutinin (HA)-Tag

Expression

Mammalian

Promoter

CMV IE94 promoter

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mVenus1-155-GGGS

Plasmid

#162610

Purpose

Allows for transcription of control mVenus fluorophore half for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

mVenus

Expression

Mammalian

Mutation

Contains aa1-155 of mVenus

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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rag2:MYRFL_CE_pISceI

Plasmid

#223045

Purpose

Express MYRFL gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

MYRFL

Use

Express the insert(gene) in mesenchymal lineage o…

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+-Y276H PHYB-CAAX

Plasmid

#154911

Purpose

1-621 amino acids of Arabidopsis PHYB (as in construct Addgene ID 154910) with the CAAX membrane moiety tag. Tyrosine residue 276 of PHYB was mutated to Histidine.

Depositor

Clare Buckley, Jonathan Clarke

Article

Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:

Insert

Phytochrome B (PHYB Mustard Weed)

Use

Xenopus/avian/zebrafish

Tags

CAAX membrane moiety and mCherry

Expression

Mammalian

Mutation

1-621 amino acids of Arabidopsis PHYB, Tyrosine r…

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Danio alpha A-crystallin 1kb promoter/AcGFP

Plasmid

#98096

Purpose

aA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos

Depositor

Mason Posner

Article

Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

Insert

Alpha A-crystallin promoter 1 kb fragment, Danio rerio (cryaa Zebrafish)

Use

Gfp expressing

Tags

GFP

Promoter

Danio alpha A-crystallin 1 kb fragment (-1028/-1)

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pAG657 H2B-pFAST

Plasmid

#172862

Purpose

Expresses pFAST fused to zebrafish histone H2B in mammalian cells

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

H2B-pFAST

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pMTB2-Avi-Cerulean-RanGAP

Plasmid

#79878

Purpose

Beta-actin2 promoter driving N-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)

Tags

Avi

Expression

Bacterial

Promoter

Beta-actin2

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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ACVR1-TAAS-R206H

Plasmid

#206184

Purpose

in vitro transcription of RNA encoding human ACVR1 with mutations S190A, S192A, and R206H

Depositor

Mary Mullins

Article

Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

Insert

activin A receptor type 1 (ACVR1 Human)

Use

In vitro transcription

Tags

FLAG tag

Mutation

S190A, S192A, R206H

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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