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Valves Are a Conserved Feature of the Zebrafish Lymphatic System.
Shin M, Nozaki T, Idrizi F, Isogai S, Ogasawara K, Ishida K, Yuge S, Roscoe B, Wolfe SA, Fukuhara S, Mochizuki N, Deguchi T, Lawson ND
Dev Cell. 2019 Nov 4;51(3):374-386.e5. doi: 10.1016/j.devcel.2019.08.019. Epub 2019 Sep 26.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
132968p5E-6xgata2aECEbaspgata2a endothelial enhancer (x6) and basal promoter in p5E Gateway vector
132969p5E-6xgata2aECEgata2a endothelial enhancer (x6) in p5E Gateway vector
132970pCS-foxc1a-2a-nlstdTomatofull-length of foxc1a in frame with viral 2a peptide and nuclear localized tdTomato in pCS expression vector
132971pME-foxc1afull-length of foxc1a without stop codon in pmE Gateway vector
132972pME-Kaedefull-length of photoconvertible kaede with stop codon in pmE Gateway vector
132973pME-nls-sfGFPfull-length of nuclear localized sfGFP with stop codon in pmE Gateway vector
132974pTone-gata2aECE-EGFPgata2a endothelial enhancer (x6) and basal promoter driving egfp in pTol1 backbone
132975pTone-gata2aECE-nsGFPgata2a endothelial enhancer (x6) and basal promoter driving nuclear-localized sfGFP in pTol1 backbone
132976pTone-gata2aECE-Kaedegata2a endothelial enhancer (x6) and basal promoter driving kaede in pTol1 backbone
132977pME-gata2afull-length of gata2a without stop codon in pmE Gateway vector
132978pDR-nfatc1_sgRNA2nfatc1 sgRNA targeting to "5'-GTGGGAGCTCCATTGGATCG-3" in pDR274
132979pDR-itga9_sgRNA1itga9 sgRNA targeting to "5'-GAATGACGGAGCTCTCTACG-3" in pDR274
132980pDR-itga9_sgRNA4itga9 sgRNA targeting to "5'-TCCGCTGCCAGCCAGCCGG-3" in pDR274
132981pDR-itga9_sgRNA7itga9 sgRNA targeting to "5'-CCCTCCAGCCTTCCATATG-3" in pDR274
132985pCS-nlstdTomatofull length of nuclear localized tdTomato in pCS expression vector

Antibodies from Article