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pTone-gata2aECE-Kaede
(Plasmid #132976)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132976 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 132976-D50 50 μg of DNA in Tris buffer $495
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DNA 132976-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pTol1 vector
  • Backbone size w/o insert (bp) 3299
  • Total vector size (bp) 4407
  • Vector type
    Unspecified ; Transposon-mediated

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Kaede
  • Species
    Trachyphyllia geoffroyi
  • Insert Size (bp)
    678

Cloning Information for Gene/Insert 1

  • Cloning method Gateway Cloning
  • 5′ sequencing primer not appreciable
  • 3′ sequencing primer not appreciable
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    gata2a endothelial enhancer (x6) and a carp b-actin basal promoter
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    339

Cloning Information for Gene/Insert 2

  • Cloning method Gateway Cloning
  • 5′ sequencing primer not appreciable
  • 3′ sequencing primer not appreciable
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTone-gata2aECE-Kaede was a gift from Nathan Lawson (Addgene plasmid # 132976 ; http://n2t.net/addgene:132976 ; RRID:Addgene_132976)

  • For your References section:

    Valves Are a Conserved Feature of the Zebrafish Lymphatic System. Shin M, Nozaki T, Idrizi F, Isogai S, Ogasawara K, Ishida K, Yuge S, Roscoe B, Wolfe SA, Fukuhara S, Mochizuki N, Deguchi T, Lawson ND. Dev Cell. 2019 Nov 4;51(3):374-386.e5. doi: 10.1016/j.devcel.2019.08.019. Epub 2019 Sep 26. 10.1016/j.devcel.2019.08.019 PubMed 31564611
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