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CIB81-pmGFP

Plasmid

#75366

Purpose

Expresses aa1-81 of Arabidopsis CIB1 fused to EGFP with CaaX membrane tag on end

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CIB1 (CIB1 Mustard Weed)

Use

Tags

prenylated EGFP

Expression

Mammalian

Mutation

residues 1-81 of CIB1

Promoter

CMV

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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CRY2PHR(W349R)-mCherry

Plasmid

#75370

Purpose

Expresses fusion of Arabidopsis CRY2 (residues 1-498, W349R fast-cycling mutant) fused to mCherry

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CRY2 residues 1-498 (CRY2 Mustard Weed)

Use

Tags

mCherry

Expression

Mammalian

Mutation

W349R

Promoter

CMV

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pEG BacMam C term 3C eGFP StrepII

Plasmid

#160686

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Use

Tags

3C eGFP StrepII

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-GFP (PX461)

Plasmid

#48140

Purpose

Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9n

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Promoter

Cbh

Available since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pPUR-hU6-Sirius-8XMS2-mU6-Sirius-8XPP7

Plasmid

#121944

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

hU6-Sirius-8XMS2-mU6-Sirius-8XPP7 Cassette

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Mutation

Promoter

U6 promoters

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pEG BacMam C term 3C eGFP His8

Plasmid

#160687

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Use

Tags

3C eGFP His8

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pJRH-1180 U6-reci Gag-pol v2

Plasmid

#201915

Purpose

Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Gag-pol

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pJRH-1179 U6-reci Gag-Cas9 v2

Plasmid

#201914

Purpose

Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Gag-Cas9 v2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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hisSUMO-CTCF(F1-F9)

Plasmid

#102859

Purpose

Express DNA binding domain of mouse CTCF in E. coli

Depositor

Gary Stormo

Article

Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

Insert

CTCF (Ctcf Mouse)

Use

Tags

hisSUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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TR-Csy4-R115A/R119A

Plasmid

#80696

Purpose

Expresses Csy4 with R115A and R119A mutations from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

Csy4-R115A/R119A

Use

AAV

Tags

Expression

Mammalian

Mutation

Arg115 and Arg119 both mutated to Ala

Promoter

CBA

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-JMJD2B

Plasmid

#24181

Purpose

Mammalian expression of human JMJD2B with HA tag

Depositor

Kristian Helin

Article

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

JMJD2B (KDM4B Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pLX303-ABI1-dCas9

Plasmid

#154474

Purpose

Expresses ABI1 fused to the N terminus of dCas9

Depositor

Mikko Taipale

Article

Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

Insert

ABI1 (ABI1 Mustard Weed)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Includes ABI1 aa 126-423, D143A

Promoter

CMV

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pLV_hEF1a_rtTA3

Plasmid

#61472

Purpose

3rd generation lentiviral vector; constitutive expression of rtTA3 (TetON system)

Depositor

Ron Weiss

Article

Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

Insert

rtTA3

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hEF1a

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCAGGS-N-TEV-Twin-Strep

Plasmid

#202526

Purpose

Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Use

Tags

TEV cleavable site and Twin-strep-tag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-C-TEV-Twin-Strep

Plasmid

#202527

Purpose

Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Use

Tags

TEV cleavable site and Twin-Strep-tag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pRV∆L-5Cre

Plasmid

#182964

Purpose

The 3rd generation of rabies vector expressing Cre recombinase

Depositor

Ian Wickersham

Article

Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

CRE recombinase

Use

Rabies

Tags

Expression

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pGST2-TSG101 (2-145)

Plasmid

#184810

Purpose

Bacterial expression of TSG101 UEV domain (aa 2-145)

Depositor

James Hurley

Article

Im et al Structure. 2010 Nov 10. 18(11):1536-47.

Insert

TSG101 (TSG101 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 2-145 only

Promoter

tac

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Tollip

Plasmid

#178050

Purpose

GFP fused to human Tollip cDNA. Localisation and expression in mammalian cells.

Depositor

David Tumbarello

Article

Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

Insert

Toll-Interacting Protein (TOLLIP Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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ATF4 6: mCD36.GFP

Plasmid

#21853

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

mCD36 (Cd36 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Only the first 13 AA of CD36 are fused to GFP

Promoter

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pEG BacMam N term StrepII eGFP 3C

Plasmid

#160683

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Use

Tags

StrepII eGFP 3C

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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