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T2A-mCherry-P2A-NTRv2-pA-ORF-2

Plasmid

#241197

Purpose

Middle entry vector for mini-Golden subcloning platform; mCherry; NTR-v2

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry; NTR-v2

Use

Mini-golden

Mutation

TCGC linker

Available since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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SA-EGFP-pA

Plasmid

#241211

Purpose

Middle entry vector for mini-Golden subcloning platform; eGFP

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

eGFP

Use

Mini-golden

Mutation

TCGC linker

Available since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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linker-P2A-lifeact-mNeongreen-ORF-3

Plasmid

#241174

Purpose

Middle entry vector for mini-Golden subcloning platform; mNeongreen

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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memb-mNeongreen

Plasmid

#241175

Purpose

Middle entry vector for mini-Golden subcloning platform; mNeongreen

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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linker-P2A-mNeongreen-ORF1

Plasmid

#241176

Purpose

Middle entry vector for mini-Golden subcloning platform; mNeongreen

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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linker-P2A-mNeongreen-ORF2

Plasmid

#241177

Purpose

Middle entry vector for mini-Golden subcloning platform; mNeongreen

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
linker-P2A-mNeongreen-ORF3

Plasmid

#241178

Purpose

Middle entry vector for mini-Golden subcloning platform; mNeongreen

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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FRT-acrymCherry-FRT

Plasmid

#241179

Purpose

Middle entry vector for mini-Golden subcloning platform; acry-mCherry

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

acry-mCherry

Use

Mini-golden

Mutation

TCGC linker

Available since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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linker-T2A-NTRv2-linker-P2A-mNeonGreen ORF1

Plasmid

#241180

Purpose

Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

NTRv2; mNeonGreen

Use

Mini-golden

Mutation

TCGC linker

Available since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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linker-T2A-NTRv2-linker-P2A-mNeonGreen ORF2

Plasmid

#241181

Purpose

Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

NTRv2; mNeonGreen

Use

Mini-golden

Mutation

TCGC linker

Available since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_mCherry-pA

Plasmid

#200521

Purpose

Mini circle middle entry plasmid for mCherry- pA with flanking BsaI cut sites

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry

Use

Mini-golden

Available since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_BFP-pA

Plasmid

#200533

Purpose

mini circle middle entry plasmid for BFP-pA with flanking BsaI cut sites

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP

Use

Mini-golden

Available since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLL5.0_shRNAHsCavin1_Del4UC1_EGFP

Plasmid

#229691

Purpose

Lentiviral expression of shRNA targeting Cavin1 + reconstitution of zebrafish mutant Cavin1 (Del4UC1)

Depositor

Alpha Yap

Publication

Brooks et al Mol Biol Cell. 2023 Nov 1;34(12):ar120.

Inserts

shRNA for hCavin1 (CAVIN1 Human)
mutant cavin1b (Del4UC1) (cavin1b Zebrafish)

Use

Lentiviral

Tags

EGFP

Mutation

Del4UC1

Promoter

LTR viral promoter

Available since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pLL5.0_shRNAHsCavin1_DrCavin1b_EGFP

Plasmid

#229690

Purpose

Lentiviral expression of shRNA targeting Cavin1 + reconstitution of zebrafish Cavin1 (Cavin1 rescue)

Depositor

Alpha Yap

Publication

Brooks et al Mol Biol Cell. 2023 Nov 1;34(12):ar120.

Inserts

shRNA for hCavin1 (CAVIN1 Human)
cavin1b (cavin1b Zebrafish)

Use

Lentiviral

Tags

EGFP

Promoter

LTR viral promoter

Available since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG3-drAgo2D-A

Plasmid

#221734

Purpose

Expression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Publication

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-A (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Mutation

D683A in active site

Promoter

CMV

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG3-drAgo2Y-F

Plasmid

#221732

Purpose

Expression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Publication

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2Y-F (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Mutation

Y680F in active site

Promoter

CMV

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG3-drAgo2D-E

Plasmid

#221731

Purpose

Expression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Publication

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-E (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Mutation

D651E in active site

Promoter

CMV

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG3-drAgo2

Plasmid

#221730

Purpose

Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Publication

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2 (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsAgo2F-Y

Plasmid

#221722

Purpose

Expression of human Ago2 with F666Y mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Publication

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2F-Y (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Mutation

F666Y in active site

Promoter

simian CMV IE94

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsAgo2E-D

Plasmid

#221721

Purpose

Expression of human Ago2 with E637D mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Publication

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2E-D (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Mutation

E637D in active site

Promoter

simian CMV IE94

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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