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pPRISM-gal4-VP16-gcry-mRFP

Plasmid

#117778

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pGTag-NLS-eGFP-B-actin

Plasmid

#117812

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

eGFP

Use

Synthetic Biology

Tags

NLS

Expression

Mutation

Promoter

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-Stop-cmlc2-eGFP

Plasmid

#117776

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

3x3 Stop sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-Stop-cmlc2-mRFP

Plasmid

#117775

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

3x3 Stop sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-Stop-gcry-eGFP

Plasmid

#117773

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

3x3 Stop sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-Stop-cmlc2-TagBFP

Plasmid

#117774

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

3x3 Stop sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pET28b-Hf-RfxCas13d-His

Plasmid

#234480

Purpose

Plasmid for bacterial expression and purification of High-fidelity RfxCas13d protein (human codon-optimized)

Depositor

Miguel Angel Moreno-Mateos

Article

Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

Insert

RfxCas13d

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

Changed four alanine to valine in positions 134, …

Promoter

Available since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:FRS2_CE_pISceI

Plasmid

#223040

Purpose

Express FRS2 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

FRS2 (FRS2 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Tags

Expression

Mutation

Promoter

Rag2

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:CCT2_CE_pISceI

Plasmid

#223041

Purpose

Express CCT2 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

CCT2 (CCT2 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Tags

Expression

Mutation

Promoter

Rag2

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:CNOT_CE_pISceI

Plasmid

#223046

Purpose

Express CNOT2 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

CNOT2 (CNOT2 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Tags

Expression

Mutation

Promoter

Rag2

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:METTL1-S27A_CE_pISceI

Plasmid

#223048

Purpose

Express METTL1-S27A gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

METTL1-S27A (METTL1 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Tags

Expression

Mutation

S27A

Promoter

Rag2

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:METTL1-S27D_CE_pISceI

Plasmid

#223049

Purpose

Express METTL1-S27D gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

METTL1-S27D (METTL1 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Tags

Expression

Mutation

S27D

Promoter

Rag2

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:METTL1-CM_CE_pISceI

Plasmid

#223050

Purpose

Express METTL1-CM gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

METTL1-CM (METTL1 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Tags

Expression

Mutation

160-163 (LFPD>AFPA)

Promoter

Rag2

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:MARCH9_CE_pISceI

Plasmid

#223027

Purpose

Express MARCH--9 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

MARCH--9 (MARCH9 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Tags

Expression

Mutation

Promoter

Rag2

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:CYP27B1_CE_pISceI

Plasmid

#223028

Purpose

Express CYP27B1 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

CYP27B1 (CYP27B1 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Tags

Expression

Mutation

Promoter

Rag2

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:AVIL_CE_pISceI

Plasmid

#223032

Purpose

Express AVIL gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

AVIL (AVIL Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Tags

Expression

Mutation

Promoter

Rag2

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:HMGA2_CE_pISceI

Plasmid

#223034

Purpose

Express HMGA2 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

HMGA2 (HMGA2 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Tags

Expression

Mutation

Promoter

Rag2

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:CPSF6_CE_pISceI

Plasmid

#223037

Purpose

Express CPSF6 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

CPSF6 (CPSF6 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Tags

Expression

Mutation

Promoter

Rag2

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pT22Ubm5En2SRE4PCh(#302)

Plasmid

#184089

Purpose

cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

5myc-chkEn2(SR)-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

5xMyc

Expression

Mutation

chkEn2: W247S, F248R; ERT2: G400V, M543A, L544A, …

Promoter

Zf-Ubi

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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Tgfb-Induced-Enhancer:Beta-globin-minimal-promoter-EGFP:pA pDestTol2pA2

Plasmid

#220508

Purpose

TGFb-inducible fluorescent reporter (TIE:EGFP)

Depositor

Leonard Zon

Inserts

TGFb inducible enhancer
beta-globin minimal promoter
SV40 PolyA signal

Use

Zebrafish expression (tol2)

Tags

EGFP

Expression

Mutation

Promoter

Available since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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