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We narrowed to 2,837 results for: 52963

Showing: 2741 - 2760 of 2837 results

  1. lentiGuide-Puro

    Plasmid
    #52963
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Has Service
    Cloning Grade DNA
    Inserts
    S. pyogenes sgRNA cassette
    Puromycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1-a and hU6
    Available since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52963-plasmid-map-sequence-id-331247

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  5. p667-UBC-CLK4-V5-miniTurbo_IDG-K

    Plasmid
    #189913
    Purpose
    Gateway destination clone of CLK4 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    CLK4 (CLK4 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    189913-plasmid-map-sequence-id-374023

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  6. pDestTol2CG2-U6:gRNA

    Plasmid
    #63156
    Purpose
    Destination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker.
    Depositor
    Leonard Zon
    Article
    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15
    Type
    Empty backbone
    Use
    CRISPR; Tol2 destination vector for gateway
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    63156-plasmid-map-sequence-id-177022

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  7. pLeGO.sgGata1.4.RUNX1A.iG2

    Plasmid
    #181976
    Purpose
    gene knock out of mouse GATA1, overexpression of 3xFLAG-RUNX1A (human)
    Depositor
    Jan-Henning Klusmann
    Inserts
    GATA1 gRNA: CCTAGACCAGGAAAATCCAT (Gata1 Mouse)
    RUNX1A (RUNX1 Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    Promoter
    Available since
    April 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181976-plasmid-map-sequence-id-358965

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  11. pME-Cas9-T2A-GFP

    Plasmid
    #63155
    Purpose
    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.
    Depositor
    Leonard Zon
    Article
    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15
    Insert
    Cas9-T2A-GFP
    Use
    CRISPR; Tol2 middle entry vector for gateway
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    63155-plasmid-map-sequence-id-220487

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  20. pMSCV-RUNX1-4A

    Plasmid
    #238534
    Purpose
    Used for overexpression of human RUNX1 in mammalian cells. Contains mutations that prevent phosphorylation at some sites of the translated protein.
    Depositor
    Diane Krause
    Article
    Kwon et al Blood. 2024 Oct 24;144(17):1800-1812. do
    Inserts
    RUNX1 (RUNX1 Human)
    Puromycin
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    S249A, S266A, T273A, S276A
    Promoter
    MSCV and PGK
    Available since
    June 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    238534-plasmid-map-sequence-id-476287

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Showing: 2741 - 2760 of 2837 results