lentiGuide-Puro Citations (520)

Originally described in: Improved vectors and genome-wide libraries for CRISPR screening.
Sanjana NE, Shalem O, Zhang F Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. PubMed Journal

Articles Citing lentiGuide-Puro

Articles
Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. PubMed Associated Plasmids lentiCRISPR v2 lentiCas9-Blast lentiGuide-Puro lentiCas9n(D10A)-Blast
Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation. Doench JG, Hartenian E, Graham DB, Tothova Z, Hegde M, Smith I, Sullender M, Ebert BL, Xavier RJ, Root DE. Nat Biotechnol. 2014 Sep 3. doi: 10.1038/nbt.3026. PubMed Associated Plasmids pXPR_011 pLX_311-Cas9
TRAF1 Coordinates Polyubiquitin Signaling to Enhance Epstein-Barr Virus LMP1-Mediated Growth and Survival Pathway Activation. Greenfeld H, Takasaki K, Walsh MJ, Ersing I, Bernhardt K, Ma Y, Fu B, Ashbaugh CW, Cabo J, Mollo SB, Zhou H, Li S, Gewurz BE. PLoS Pathog. 2015 May 21;11(5):e1004890. doi: 10.1371/journal.ppat.1004890. eCollection 2015 May. PubMed
Cell death during crisis is mediated by mitotic telomere deprotection. Hayashi MT, Cesare AJ, Rivera T, Karlseder J. Nature. 2015 Jun 25;522(7557):492-6. doi: 10.1038/nature14513. PubMed
A Genome-wide CRISPR Screen in Primary Immune Cells to Dissect Regulatory Networks. Parnas O, Jovanovic M, Eisenhaure TM, Herbst RH, Dixit A, Ye CJ, Przybylski D, Platt RJ, Tirosh I, Sanjana NE, Shalem O, Satija R, Raychowdhury R, Mertins P, Carr SA, Zhang F, Hacohen N, Regev A. Cell. 2015 Jul 30;162(3):675-86. doi: 10.1016/j.cell.2015.06.059. Epub 2015 Jul 16. PubMed
BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis. Canver MC, Smith EC, Sher F, Pinello L, Sanjana NE, Shalem O, Chen DD, Schupp PG, Vinjamur DS, Garcia SP, Luc S, Kurita R, Nakamura Y, Fujiwara Y, Maeda T, Yuan GC, Zhang F, Orkin SH, Bauer DE. Nature. 2015 Sep 16. doi: 10.1038/nature15521. PubMed Associated Plasmids lentiCas9-Venus lentiGuide-Crimson
Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease. Dahlman JE, Abudayyeh OO, Joung J, Gootenberg JS, Zhang F, Konermann S. Nat Biotechnol. 2015 Oct 5;33(11):1159-1161. doi: 10.1038/nbt.3390. PubMed
DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs. Aparicio-Prat E, Arnan C, Sala I, Bosch N, Guigo R, Johnson R. BMC Genomics. 2015 Oct 23;16(1):846. doi: 10.1186/s12864-015-2086-z. PubMed Associated Plasmids pDECKO_GFP pDECKO_Malat1_A pDECKO_Malat1_B pDECKO_Malat1_C pDECKO_Malat1_D More ...
The NF-kappaB subunit c-Rel regulates Bach2 tumour suppressor expression in B-cell lymphoma. Hunter JE, Butterworth JA, Zhao B, Sellier H, Campbell KJ, Thomas HD, Bacon CM, Cockell SJ, Gewurz BE, Perkins ND. Oncogene. 2015 Nov 2. doi: 10.1038/onc.2015.399. PubMed
Identification of focally amplified lineage-specific super-enhancers in human epithelial cancers. Zhang X, Choi PS, Francis JM, Imielinski M, Watanabe H, Cherniack AD, Meyerson M. Nat Genet. 2016 Feb;48(2):176-82. doi: 10.1038/ng.3470. Epub 2015 Dec 14. PubMed
Enhanced Store-Operated Calcium Entry Leads to Striatal Synaptic Loss in a Huntington's Disease Mouse Model. Wu J, Ryskamp DA, Liang X, Egorova P, Zakharova O, Hung G, Bezprozvanny I. J Neurosci. 2016 Jan 6;36(1):125-41. doi: 10.1523/JNEUROSCI.1038-15.2016. PubMed
CRISPR-Cas9 Can Inhibit HIV-1 Replication but NHEJ Repair Facilitates Virus Escape. Wang G, Zhao N, Berkhout B, Das AT. Mol Ther. 2016 Mar;24(3):522-6. doi: 10.1038/mt.2016.24. Epub 2016 Jan 22. PubMed
Single Guide RNA Library Design and Construction. Wang T, Lander ES, Sabatini DM. Cold Spring Harb Protoc. 2016 Mar 1;2016(3):pdb.prot090803. doi: 10.1101/pdb.prot090803. PubMed
Mutant Calreticulin Requires Both Its Mutant C-terminus and the Thrombopoietin Receptor for Oncogenic Transformation. Elf S, Abdelfattah NS, Chen E, Perales-Paton J, Rosen EA, Ko A, Peisker F, Florescu N, Giannini S, Wolach O, Morgan EA, Tothova Z, Losman JA, Schneider RK, Al-Shahrour F, Mullally A. Cancer Discov. 2016 Apr;6(4):368-81. doi: 10.1158/2159-8290.CD-15-1434. Epub 2016 Mar 7. PubMed
Novel Small-Molecule CX3CR1 Antagonist Impairs Metastatic Seeding and Colonization of Breast Cancer Cells. Shen F, Zhang Y, Jernigan DL, Feng X, Yan J, Garcia FU, Meucci O, Salvino JM, Fatatis A. Mol Cancer Res. 2016 Jun;14(6):518-27. doi: 10.1158/1541-7786.MCR-16-0013. Epub 2016 Mar 21. PubMed
CRISPR knockout screening outperforms shRNA and CRISPRi in identifying essential genes. Evers B, Jastrzebski K, Heijmans JP, Grernrum W, Beijersbergen RL, Bernards R. Nat Biotechnol. 2016 Jun;34(6):631-3. doi: 10.1038/nbt.3536. Epub 2016 Apr 25. PubMed
RBFOX1 and RBFOX2 are dispensable in iPSCs and iPSC-derived neurons and do not contribute to neural-specific paternal UBE3A silencing. Chen PF, Hsiao JS, Sirois CL, Chamberlain SJ. Sci Rep. 2016 May 5;6:25368. doi: 10.1038/srep25368. PubMed
A human genome-wide loss-of-function screen identifies effective chikungunya antiviral drugs. Karlas A, Berre S, Couderc T, Varjak M, Braun P, Meyer M, Gangneux N, Karo-Astover L, Weege F, Raftery M, Schonrich G, Klemm U, Wurzlbauer A, Bracher F, Merits A, Meyer TF, Lecuit M. Nat Commun. 2016 May 12;7:11320. doi: 10.1038/ncomms11320. PubMed
Modeling invasive lobular breast carcinoma by CRISPR/Cas9-mediated somatic genome editing of the mammary gland. Annunziato S, Kas SM, Nethe M, Yucel H, Del Bravo J, Pritchard C, Bin Ali R, van Gerwen B, Siteur B, Drenth AP, Schut E, van de Ven M, Boelens MC, Klarenbeek S, Huijbers IJ, van Miltenburg MH, Jonkers J. Genes Dev. 2016 Jun 15;30(12):1470-80. doi: 10.1101/gad.279190.116. PubMed
A USP28-53BP1-p53-p21 signaling axis arrests growth after centrosome loss or prolonged mitosis. Lambrus BG, Daggubati V, Uetake Y, Scott PM, Clutario KM, Sluder G, Holland AJ. J Cell Biol. 2016 Jul 18;214(2):143-53. doi: 10.1083/jcb.201604054. PubMed
Treatment with an SLC12A1 antagonist inhibits tumorigenesis in a subset of hepatocellular carcinomas. Teng F, Guo M, Liu F, Wang C, Dong J, Zhang L, Zou Y, Chen R, Sun K, Fu H, Fu Z, Guo W, Ding G. Oncotarget. 2016 Aug 16;7(33):53571-53582. doi: 10.18632/oncotarget.10670. PubMed
Histone H3K27 Trimethylation Modulates 5-Fluorouracil Resistance by Inhibiting PU.1 Binding to the DPYD Promoter. Wu R, Nie Q, Tapper EE, Jerde CR, Dunlap GS, Shrestha S, Elraiyah TA, Offer SM, Diasio RB. Cancer Res. 2016 Nov 1;76(21):6362-6373. Epub 2016 Aug 30. PubMed
Challenges of CRISPR/Cas9 applications for long non-coding RNA genes. Goyal A, Myacheva K, Gross M, Klingenberg M, Duran Arque B, Diederichs S. Nucleic Acids Res. 2016 Sep 30. pii: gkw883. PubMed
Orphan G protein-coupled receptor GPRC5A modulates integrin beta1-mediated epithelial cell adhesion. Bulanova DR, Akimov YA, Rokka A, Laajala TD, Aittokallio T, Kouvonen P, Pellinen T, Kuznetsov SG. Cell Adh Migr. 2016 Oct 7:0. PubMed
The sigma-1 receptor mediates the beneficial effects of pridopidine in a mouse model of Huntington disease. Ryskamp D, Wu J, Geva M, Kusko R, Grossman I, Hayden M, Bezprozvanny I. Neurobiol Dis. 2017 Jan;97(Pt A):46-59. doi: 10.1016/j.nbd.2016.10.006. Epub 2016 Nov 3. PubMed
DPP8 and DPP9 inhibition induces pro-caspase-1-dependent monocyte and macrophage pyroptosis. Okondo MC, Johnson DC, Sridharan R, Go EB, Chui AJ, Wang MS, Poplawski SE, Wu W, Liu Y, Lai JH, Sanford DG, Arciprete MO, Golub TR, Bachovchin WW, Bachovchin DA. Nat Chem Biol. 2017 Jan;13(1):46-53. doi: 10.1038/nchembio.2229. Epub 2016 Nov 7. PubMed
Alternative splicing of EZH2 pre-mRNA by SF3B3 contributes to the tumorigenic potential of renal cancer. Chen K, Xiao H, Zeng J, Yu G, Zhou H, Huang C, Yao W, Xiao W, Hu J, Guan W, Wu L, Huang J, Huang Q, Xu H, Ye Z. Clin Cancer Res. 2016 Nov 22. pii: clincanres.2020.2016. PubMed
A Combinatorial CRISPR-Cas9 Attack on HIV-1 DNA Extinguishes All Infectious Provirus in Infected T Cell Cultures. Wang G, Zhao N, Berkhout B, Das AT. Cell Rep. 2016 Dec 13;17(11):2819-2826. doi: 10.1016/j.celrep.2016.11.057. PubMed
Dissecting Immune Circuits by Linking CRISPR-Pooled Screens with Single-Cell RNA-Seq. Jaitin DA, Weiner A, Yofe I, Lara-Astiaso D, Keren-Shaul H, David E, Salame TM, Tanay A, van Oudenaarden A, Amit I. Cell. 2016 Dec 15;167(7):1883-1896.e15. doi: 10.1016/j.cell.2016.11.039. PubMed Associated Plasmids CRISPseq-BFP-backbone CRISPseq-mCherry-backbone
In vivo high-throughput profiling of CRISPR-Cpf1 activity. Kim HK, Song M, Lee J, Menon AV, Jung S, Kang YM, Choi JW, Woo E, Koh HC, Nam JW, Kim H. Nat Methods. 2016 Dec 19. doi: 10.1038/nmeth.4104. PubMed Associated Plasmids Lenti-AsCpf1-Blast Lenti-LbCpf1-Blast Lenti_gRNA-Puro
MAPK signaling promotes axonal degeneration by speeding the turnover of the axonal maintenance factor NMNAT2. Walker LJ, Summers DW, Sasaki Y, Brace EJ, Milbrandt J, DiAntonio A. Elife. 2017 Jan 17;6. doi: 10.7554/eLife.22540. PubMed
Pooled CRISPR screening with single-cell transcriptome readout. Datlinger P, Rendeiro AF, Schmidl C, Krausgruber T, Traxler P, Klughammer J, Schuster LC, Kuchler A, Alpar D, Bock C. Nat Methods. 2017 Jan 18. doi: 10.1038/nmeth.4177. PubMed Associated Plasmids CROPseq-Guide-Puro
Challenges of CRISPR/Cas9 applications for long non-coding RNA genes. Goyal A, Myacheva K, Gross M, Klingenberg M, Duran Arque B, Diederichs S. Nucleic Acids Res. 2017 Feb 17;45(3):e12. doi: 10.1093/nar/gkw883. PubMed
Variant-aware saturating mutagenesis using multiple Cas9 nucleases identifies regulatory elements at trait-associated loci. Canver MC, Lessard S, Pinello L, Wu Y, Ilboudo Y, Stern EN, Needleman AJ, Galacteros F, Brugnara C, Kutlar A, McKenzie C, Reid M, Chen DD, Das PP, A Cole M, Zeng J, Kurita R, Nakamura Y, Yuan GC, Lettre G, Bauer DE, Orkin SH. Nat Genet. 2017 Feb 20. doi: 10.1038/ng.3793. PubMed Associated Plasmids lenti-Cas9-VQR-Blast lenti-Cas9-VQR-GFP_activity_reporter
TIRR regulates 53BP1 by masking its histone methyl-lysine binding function. Drane P, Brault ME, Cui G, Meghani K, Chaubey S, Detappe A, Parnandi N, He Y, Zheng XF, Botuyan MV, Kalousi A, Yewdell WT, Munch C, Harper JW, Chaudhuri J, Soutoglou E, Mer G, Chowdhury D. Nature. 2017 Mar 9;543(7644):211-216. doi: 10.1038/nature21358. Epub 2017 Feb 27. PubMed
Glucose Deprivation Induces ATF4-Mediated Apoptosis through TRAIL Death Receptors. Iurlaro R, Puschel F, Leon-Annicchiarico CL, O'Connor H, Martin SJ, Palou-Gramon D, Lucendo E, Munoz-Pinedo C. Mol Cell Biol. 2017 May 2;37(10). pii: e00479-16. doi: 10.1128/MCB.00479-16. Print 2017 May 15. PubMed
Combinatorial CRISPR-Cas9 screens for de novo mapping of genetic interactions. Shen JP, Zhao D, Sasik R, Luebeck J, Birmingham A, Bojorquez-Gomez A, Licon K, Klepper K, Pekin D, Beckett AN, Sanchez KS, Thomas A, Kuo CC, Du D, Roguev A, Lewis NE, Chang AN, Kreisberg JF, Krogan N, Qi L, Ideker T, Mali P. Nat Methods. 2017 Jun;14(6):573-576. doi: 10.1038/nmeth.4225. Epub 2017 Mar 20. PubMed
Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening. Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F. Nat Protoc. 2017 Apr;12(4):828-863. doi: 10.1038/nprot.2017.016. Epub 2017 Mar 23. PubMed Associated Plasmids lenti sgRNA(MS2)_puro optimized backbone lentiSAMv2 lentiMPH v2
USP9X regulates centrosome duplication and promotes breast carcinogenesis. Li X, Song N, Liu L, Liu X, Ding X, Song X, Yang S, Shan L, Zhou X, Su D, Wang Y, Zhang Q, Cao C, Ma S, Yu N, Yang F, Wang Y, Yao Z, Shang Y, Shi L. Nat Commun. 2017 Mar 31;8:14866. doi: 10.1038/ncomms14866. PubMed
Pyroptosis and Apoptosis Pathways Engage in Bidirectional Crosstalk in Monocytes and Macrophages. Taabazuing CY, Okondo MC, Bachovchin DA. Cell Chem Biol. 2017 Apr 20;24(4):507-514.e4. doi: 10.1016/j.chembiol.2017.03.009. Epub 2017 Apr 6. PubMed
Epstein-Barr virus ensures B cell survival by uniquely modulating apoptosis at early and late times after infection. Price AM, Dai J, Bazot Q, Patel L, Nikitin PA, Djavadian R, Winter PS, Salinas CA, Barry AP, Wood KC, Johannsen EC, Letai A, Allday MJ, Luftig MA. Elife. 2017 Apr 20;6. pii: e22509. doi: 10.7554/eLife.22509. PubMed
Proteomic mapping of cytosol-facing outer mitochondrial and ER membranes in living human cells by proximity biotinylation. Hung V, Lam SS, Udeshi ND, Svinkina T, Guzman G, Mootha VK, Carr SA, Ting AY. Elife. 2017 Apr 25;6. pii: e24463. doi: 10.7554/eLife.24463. PubMed Associated Plasmids pLX_TRC208-SYNJ2BP-V5 pcDNA4 HisMax-V5-GFP-RRBP1 lentiGuide-RRBP1-1 lentiGuide-RRBP1-2 pLX304-Flag-APEX2-NES More ...
High-content analysis screening for cell cycle regulators using arrayed synthetic crRNA libraries. Strezoska Z, Perkett MR, Chou ET, Maksimova E, Anderson EM, McClelland S, Kelley ML, Vermeulen A, Smith AVB. J Biotechnol. 2017 Jun 10;251:189-200. doi: 10.1016/j.jbiotec.2017.04.017. Epub 2017 Apr 23. PubMed
Genome-wide association study of red blood cell traits in Hispanics/Latinos: The Hispanic Community Health Study/Study of Latinos. Hodonsky CJ, Jain D, Schick UM, Morrison JV, Brown L, McHugh CP, Schurmann C, Chen DD, Liu YM, Auer PL, Laurie CA, Taylor KD, Browning BL, Li Y, Papanicolaou G, Rotter JI, Kurita R, Nakamura Y, Browning SR, Loos RJF, North KE, Laurie CC, Thornton TA, Pankratz N, Bauer DE, Sofer T, Reiner AP. PLoS Genet. 2017 Apr 28;13(4):e1006760. doi: 10.1371/journal.pgen.1006760. eCollection 2017 Apr. PubMed
CRISPR/Cas9 Screens Reveal Epstein-Barr Virus-Transformed B Cell Host Dependency Factors. Ma Y, Walsh MJ, Bernhardt K, Ashbaugh CW, Trudeau SJ, Ashbaugh IY, Jiang S, Jiang C, Zhao B, Root DE, Doench JG, Gewurz BE. Cell Host Microbe. 2017 May 10;21(5):580-591.e7. doi: 10.1016/j.chom.2017.04.005. PubMed
LDB1-mediated enhancer looping can be established independent of mediator and cohesin. Krivega I, Dean A. Nucleic Acids Res. 2017 May 18. doi: 10.1093/nar/gkx433. PubMed
Complementary information derived from CRISPR Cas9 mediated gene deletion and suppression. Rosenbluh J, Xu H, Harrington W, Gill S, Wang X, Vazquez F, Root DE, Tsherniak A, Hahn WC. Nat Commun. 2017 May 23;8:15403. doi: 10.1038/ncomms15403. PubMed Associated Plasmids pLX_311-KRAB-dCas9
The ubiquitin ligase STUB1 regulates stability and activity of RUNX1 and RUNX1-RUNX1T1. Yonezawa T, Takahashi H, Shikata S, Liu X, Tamura M, Asada S, Fukushima T, Fukuyama T, Tanaka Y, Sawasaki T, Kitamura T, Goyama S. J Biol Chem. 2017 Jul 28;292(30):12528-12541. doi: 10.1074/jbc.M117.785675. Epub 2017 May 23. PubMed
RNA-Guided Activation of Pluripotency Genes in Human Fibroblasts. Xiong K, Zhou Y, Blichfeld KA, Hyttel P, Bolund L, Freude KK, Luo Y. Cell Reprogram. 2017 Jun;19(3):189-198. doi: 10.1089/cell.2017.0006. PubMed
Aldolase B Overexpression is Associated with Poor Prognosis and Promotes Tumor Progression by Epithelial-Mesenchymal Transition in Colorectal Adenocarcinoma. Li Q, Li Y, Xu J, Wang S, Xu Y, Li X, Cai S. Cell Physiol Biochem. 2017;42(1):397-406. doi: 10.1159/000477484. Epub 2017 May 25. PubMed
Seed-effect modeling improves the consistency of genome-wide loss-of-function screens and identifies synthetic lethal vulnerabilities in cancer cells. Jaiswal A, Peddinti G, Akimov Y, Wennerberg K, Kuznetsov S, Tang J, Aittokallio T. Genome Med. 2017 Jun 1;9(1):51. doi: 10.1186/s13073-017-0440-2. PubMed
Chromatin accessibility and guide sequence secondary structure affect CRISPR-Cas9 gene editing efficiency. Jensen KT, Floe L, Petersen TS, Huang J, Xu F, Bolund L, Luo Y, Lin L. FEBS Lett. 2017 Jul;591(13):1892-1901. doi: 10.1002/1873-3468.12707. Epub 2017 Jun 28. PubMed
CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion. Mou H, Smith JL, Peng L, Yin H, Moore J, Zhang XO, Song CQ, Sheel A, Wu Q, Ozata DM, Li Y, Anderson DG, Emerson CP, Sontheimer EJ, Moore MJ, Weng Z, Xue W. Genome Biol. 2017 Jun 14;18(1):108. doi: 10.1186/s13059-017-1237-8. PubMed
Optogenetic activation of Plexin-B1 reveals contact repulsion between osteoclasts and osteoblasts. Deb Roy A, Yin T, Choudhary S, Rodionov V, Pilbeam CC, Wu YI. Nat Commun. 2017 Jun 21;8:15831. doi: 10.1038/ncomms15831. PubMed
A module for Rac temporal signal integration revealed with optogenetics. Graziano BR, Gong D, Anderson KE, Pipathsouk A, Goldberg AR, Weiner OD. J Cell Biol. 2017 Aug 7;216(8):2515-2531. doi: 10.1083/jcb.201604113. Epub 2017 Jul 7. PubMed
CRISPR/Cas9-Mediated Scanning for Regulatory Elements Required for HPRT1 Expression via Thousands of Large, Programmed Genomic Deletions. Gasperini M, Findlay GM, McKenna A, Milbank JH, Lee C, Zhang MD, Cusanovich DA, Shendure J. Am J Hum Genet. 2017 Aug 3;101(2):192-205. doi: 10.1016/j.ajhg.2017.06.010. Epub 2017 Jul 14. PubMed
An erythroid-specific ATP2B4 enhancer mediates red blood cell hydration and malaria susceptibility. Lessard S, Gatof ES, Beaudoin M, Schupp PG, Sher F, Ali A, Prehar S, Kurita R, Nakamura Y, Baena E, Ledoux J, Oceandy D, Bauer DE, Lettre G. J Clin Invest. 2017 Aug 1;127(8):3065-3074. doi: 10.1172/JCI94378. Epub 2017 Jul 17. PubMed
Evaluating Synthetic Activation and Repression of Neuropsychiatric-Related Genes in hiPSC-Derived NPCs, Neurons, and Astrocytes. Ho SM, Hartley BJ, Flaherty E, Rajarajan P, Abdelaal R, Obiorah I, Barretto N, Muhammad H, Phatnani HP, Akbarian S, Brennand KJ. Stem Cell Reports. 2017 Aug 8;9(2):615-628. doi: 10.1016/j.stemcr.2017.06.012. Epub 2017 Jul 27. PubMed Associated Plasmids lenti-EF1a-dCas9-VP64-Puro lenti-EF1a-dCas9-KRAB-Puro lenti-EF1a-dCas9-VPR-Puro lentiGuide-Hygro-mTagBFP2 lentiGuide-Hygro-eGFP More ...
Technical considerations for the use of CRISPR/Cas9 in hematology research. Gundry MC, Dever DP, Yudovich D, Bauer DE, Haas S, Wilkinson AC, Singbrant S. Exp Hematol. 2017 Oct;54:4-11. doi: 10.1016/j.exphem.2017.07.006. Epub 2017 Jul 27. PubMed
Mitotic progression following DNA damage enables pattern recognition within micronuclei. Harding SM, Benci JL, Irianto J, Discher DE, Minn AJ, Greenberg RA. Nature. 2017 Aug 24;548(7668):466-470. doi: 10.1038/nature23470. Epub 2017 Jul 31. PubMed
Decomposing Oncogenic Transcriptional Signatures to Generate Maps of Divergent Cellular States. Kim JW, Abudayyeh OO, Yeerna H, Yeang CH, Stewart M, Jenkins RW, Kitajima S, Konieczkowski DJ, Medetgul-Ernar K, Cavazos T, Mah C, Ting S, Van Allen EM, Cohen O, Mcdermott J, Damato E, Aguirre AJ, Liang J, Liberzon A, Alexe G, Doench J, Ghandi M, Vazquez F, Weir BA, Tsherniak A, Subramanian A, Meneses-Cime K, Park J, Clemons P, Garraway LA, Thomas D, Boehm JS, Barbie DA, Hahn WC, Mesirov JP, Tamayo P. Cell Syst. 2017 Aug 23;5(2):105-118.e9. doi: 10.1016/j.cels.2017.08.002. PubMed
Combinatorial CRISPR-Cas9 and RNA Interference Attack on HIV-1 DNA and RNA Can Lead to Cross-Resistance. Zhao N, Wang G, Das AT, Berkhout B. Antimicrob Agents Chemother. 2017 Nov 22;61(12). pii: AAC.01486-17. doi: 10.1128/AAC.01486-17. Print 2017 Dec. PubMed
BRCA2 suppresses replication stress-induced mitotic and G1 abnormalities through homologous recombination. Feng W, Jasin M. Nat Commun. 2017 Sep 13;8(1):525. doi: 10.1038/s41467-017-00634-0. PubMed
Genome-Wide CRISPR Screen for Essential Cell Growth Mediators in Mutant KRAS Colorectal Cancers. Yau EH, Kummetha IR, Lichinchi G, Tang R, Zhang Y, Rana TM. Cancer Res. 2017 Nov 15;77(22):6330-6339. doi: 10.1158/0008-5472.CAN-17-2043. Epub 2017 Sep 27. PubMed
CRISPR Genome-Wide Screening Identifies Dependence on the Proteasome Subunit PSMC6 for Bortezomib Sensitivity in Multiple Myeloma. Shi CX, Kortum KM, Zhu YX, Bruins LA, Jedlowski P, Votruba PG, Luo M, Stewart RA, Ahmann J, Braggio E, Stewart AK. Mol Cancer Ther. 2017 Dec;16(12):2862-2870. doi: 10.1158/1535-7163.MCT-17-0130. Epub 2017 Sep 27. PubMed
Somatic Superenhancer Duplications and Hotspot Mutations Lead to Oncogenic Activation of the KLF5 Transcription Factor. Zhang X, Choi PS, Francis JM, Gao GF, Campbell JD, Ramachandran A, Mitsuishi Y, Ha G, Shih J, Vazquez F, Tsherniak A, Taylor AM, Zhou J, Wu Z, Berger AC, Giannakis M, Hahn WC, Cherniack AD, Meyerson M. Cancer Discov. 2018 Jan;8(1):108-125. doi: 10.1158/2159-8290.CD-17-0532. Epub 2017 Sep 29. PubMed
The Glia-Neuron Lactate Shuttle and Elevated ROS Promote Lipid Synthesis in Neurons and Lipid Droplet Accumulation in Glia via APOE/D. Liu L, MacKenzie KR, Putluri N, Maletic-Savatic M, Bellen HJ. Cell Metab. 2017 Nov 7;26(5):719-737.e6. doi: 10.1016/j.cmet.2017.08.024. Epub 2017 Sep 28. PubMed
Genetic and Functional Drivers of Diffuse Large B Cell Lymphoma. Reddy A, Zhang J, Davis NS, Moffitt AB, Love CL, Waldrop A, Leppa S, Pasanen A, Meriranta L, Karjalainen-Lindsberg ML, Norgaard P, Pedersen M, Gang AO, Hogdall E, Heavican TB, Lone W, Iqbal J, Qin Q, Li G, Kim SY, Healy J, Richards KL, Fedoriw Y, Bernal-Mizrachi L, Koff JL, Staton AD, Flowers CR, Paltiel O, Goldschmidt N, Calaminici M, Clear A, Gribben J, Nguyen E, Czader MB, Ondrejka SL, Collie A, Hsi ED, Tse E, Au-Yeung RKH, Kwong YL, Srivastava G, Choi WWL, Evens AM, Pilichowska M, Sengar M, Reddy N, Li S, Chadburn A, Gordon LI, Jaffe ES, Levy S, Rempel R, Tzeng T, Happ LE, Dave T, Rajagopalan D, Datta J, Dunson DB, Dave SS. Cell. 2017 Oct 5;171(2):481-494.e15. doi: 10.1016/j.cell.2017.09.027. PubMed
CRISPR/Cas9 screening using unique molecular identifiers. Schmierer B, Botla SK, Zhang J, Turunen M, Kivioja T, Taipale J. Mol Syst Biol. 2017 Oct 9;13(10):945. doi: 10.15252/msb.20177834. PubMed Associated Plasmids lentiGuide-Puro.3xBsmBI lentiGuide-Puro-Chen-tracrRNA_2xBsmBI lenti-Cas9-sgHPRT1
The Epstein-Barr Virus Regulome in Lymphoblastoid Cells. Jiang S, Zhou H, Liang J, Gerdt C, Wang C, Ke L, Schmidt SCS, Narita Y, Ma Y, Wang S, Colson T, Gewurz B, Li G, Kieff E, Zhao B. Cell Host Microbe. 2017 Oct 11;22(4):561-573.e4. doi: 10.1016/j.chom.2017.09.001. PubMed
CRISPR/Cas9-mediated gene knockout is insensitive to target copy number but is dependent on guide RNA potency and Cas9/sgRNA threshold expression level. Yuen G, Khan FJ, Gao S, Stommel JM, Batchelor E, Wu X, Luo J. Nucleic Acids Res. 2017 Nov 16;45(20):12039-12053. doi: 10.1093/nar/gkx843. PubMed
Systematic identification of regulatory variants associated with cancer risk. Liu S, Liu Y, Zhang Q, Wu J, Liang J, Yu S, Wei GH, White KP, Wang X. Genome Biol. 2017 Oct 23;18(1):194. doi: 10.1186/s13059-017-1322-z. PubMed
TUBB3 overexpression has a negligible effect on the sensitivity to taxol in cultured cell lines. Tame MA, Manjon AG, Belokhvostova D, Raaijmakers JA, Medema RH. Oncotarget. 2017 May 10;8(42):71536-71547. doi: 10.18632/oncotarget.17740. eCollection 2017 Sep 22. PubMed
Growing and Genetically Manipulating Human Umbilical Cord Blood-Derived Erythroid Progenitor (HUDEP) Cell Lines. Vinjamur DS, Bauer DE. Methods Mol Biol. 2018;1698:275-284. doi: 10.1007/978-1-4939-7428-3_17. PubMed
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Ephrin receptor A2 is a functional entry receptor for Epstein-Barr virus. Chen J, Sathiyamoorthy K, Zhang X, Schaller S, Perez White BE, Jardetzky TS, Longnecker R. Nat Microbiol. 2018 Feb;3(2):172-180. doi: 10.1038/s41564-017-0081-7. Epub 2018 Jan 1. PubMed
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PERK-mediated translational control is required for collagen secretion in chondrocytes. Hisanaga S, Miyake M, Taniuchi S, Oyadomari M, Morimoto M, Sato R, Hirose J, Mizuta H, Oyadomari S. Sci Rep. 2018 Jan 15;8(1):773. doi: 10.1038/s41598-017-19052-9. PubMed
In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR-dCas9-activator transgenic mice. Zhou H, Liu J, Zhou C, Gao N, Rao Z, Li H, Hu X, Li C, Yao X, Shen X, Sun Y, Wei Y, Liu F, Ying W, Zhang J, Tang C, Zhang X, Xu H, Shi L, Cheng L, Huang P, Yang H. Nat Neurosci. 2018 Jan 15. pii: 10.1038/s41593-017-0060-6. doi: 10.1038/s41593-017-0060-6. PubMed Associated Plasmids CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyA dSV40-NLS-dCas9-HA-NLS-NLS-10xGCN4 EF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyA AAV-hSyn-mCherry-P2A-Cre-WPRE AAV-aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA
Human Pancreatic Tumor Organoids Reveal Loss of Stem Cell Niche Factor Dependence during Disease Progression. Seino T, Kawasaki S, Shimokawa M, Tamagawa H, Toshimitsu K, Fujii M, Ohta Y, Matano M, Nanki K, Kawasaki K, Takahashi S, Sugimoto S, Iwasaki E, Takagi J, Itoi T, Kitago M, Kitagawa Y, Kanai T, Sato T. Cell Stem Cell. 2018 Mar 1;22(3):454-467.e6. doi: 10.1016/j.stem.2017.12.009. Epub 2018 Jan 11. PubMed
Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells. Abdelfattah NS, Mullally A. J Vis Exp. 2018 Jan 5;(131). doi: 10.3791/56726. PubMed
Inhibition of Dpp8/9 Activates the Nlrp1b Inflammasome. Okondo MC, Rao SD, Taabazuing CY, Chui AJ, Poplawski SE, Johnson DC, Bachovchin DA. Cell Chem Biol. 2018 Mar 15;25(3):262-267.e5. doi: 10.1016/j.chembiol.2017.12.013. Epub 2018 Jan 27. PubMed
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Next generation high throughput DNA damage detection platform for genotoxic compound screening. Sykora P, Witt KL, Revanna P, Smith-Roe SL, Dismukes J, Lloyd DG, Engelward BP, Sobol RW. Sci Rep. 2018 Feb 9;8(1):2771. doi: 10.1038/s41598-018-20995-w. PubMed
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Inhibition of TRPC1-Dependent Store-Operated Calcium Entry Improves Synaptic Stability and Motor Performance in a Mouse Model of Huntington's Disease. Wu J, Ryskamp D, Birnbaumer L, Bezprozvanny I. J Huntingtons Dis. 2018;7(1):35-50. doi: 10.3233/JHD-170266. PubMed
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PI4KIIalpha regulates insulin secretion and glucose homeostasis via a PKD-dependent pathway. Zhang L, Li J, Zhang P, Gao Z, Zhao Y, Qiao X, Chen C. Biophys Rep. 2018;4(1):25-38. doi: 10.1007/s41048-018-0049-z. Epub 2018 Mar 7. PubMed
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Genome-wide CRISPR/Cas9 Screen Identifies Host Factors Essential for Influenza Virus Replication. Han J, Perez JT, Chen C, Li Y, Benitez A, Kandasamy M, Lee Y, Andrade J, tenOever B, Manicassamy B. Cell Rep. 2018 Apr 10;23(2):596-607. doi: 10.1016/j.celrep.2018.03.045. PubMed
Reciprocal Network between Cancer Stem-Like Cells and Macrophages Facilitates the Progression and Androgen Deprivation Therapy Resistance of Prostate Cancer. Huang H, Wang C, Liu F, Li HZ, Peng G, Gao X, Dong KQ, Wang HR, Kong DP, Qu M, Dai LH, Wang KJ, Zhou Z, Yang J, Yang ZY, Cheng YQ, Tian QQ, Liu D, Xu CL, Xu DF, Cui XG, Sun YH. Clin Cancer Res. 2018 Sep 15;24(18):4612-4626. doi: 10.1158/1078-0432.CCR-18-0461. Epub 2018 Apr 24. PubMed
PHF5A Epigenetically Inhibits Apoptosis to Promote Breast Cancer Progression. Zheng YZ, Xue MZ, Shen HJ, Li XG, Ma D, Gong Y, Liu YR, Qiao F, Xie HY, Lian B, Sun WL, Zhao HY, Yao L, Zuo WJ, Li DQ, Wang P, Hu X, Shao ZM. Cancer Res. 2018 Jun 15;78(12):3190-3206. doi: 10.1158/0008-5472.CAN-17-3514. Epub 2018 Apr 26. PubMed
PDLIM7 and CDH18 regulate the turnover of MDM2 during CDK4/6 inhibitor therapy-induced senescence. Klein ME, Dickson MA, Antonescu C, Qin LX, Dooley SJ, Barlas A, Manova K, Schwartz GK, Crago AM, Singer S, Koff A, Tap WD. Oncogene. 2018 Sep;37(37):5066-5078. doi: 10.1038/s41388-018-0332-y. Epub 2018 May 23. PubMed
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iTAP, a novel iRhom interactor, controls TNF secretion by policing the stability of iRhom/TACE. Oikonomidi I, Burbridge E, Cavadas M, Sullivan G, Collis B, Naegele H, Clancy D, Brezinova J, Hu T, Bileck A, Gerner C, Bolado A, von Kriegsheim A, Martin SJ, Steinberg F, Strisovsky K, Adrain C. Elife. 2018 Jun 13;7. pii: 35032. doi: 10.7554/eLife.35032. PubMed
Producing anti-inflammatory macrophages by nanoparticle-triggered clustering of mannose receptors. Gan J, Dou Y, Li Y, Wang Z, Wang L, Liu S, Li Q, Yu H, Liu C, Han C, Huang Z, Zhang J, Wang C, Dong L. Biomaterials. 2018 Sep;178:95-108. doi: 10.1016/j.biomaterials.2018.06.015. Epub 2018 Jun 14. PubMed
Identification, characterization and application of a new peptide against anterior gradient homolog 2 (AGR2). Garri C, Howell S, Tiemann K, Tiffany A, Jalali-Yazdi F, Alba MM, Katz JE, Takahashi TT, Landgraf R, Gross ME, Roberts RW, Kani K. Oncotarget. 2018 Jun 8;9(44):27363-27379. doi: 10.18632/oncotarget.25221. eCollection 2018 Jun 8. PubMed
CK1alpha and IRF4 are essential and independent effectors of immunomodulatory drugs in primary effusion lymphoma. Patil A, Manzano M, Gottwein E. Blood. 2018 Aug 9;132(6):577-586. doi: 10.1182/blood-2018-01-828418. Epub 2018 Jun 28. PubMed Associated Plasmids pLCE-P2A-Puro pLC-Flag-CSNK1A1 WT-Puro pLC-Flag-CSNK1A1 G40N-Puro pLC-Flag-CSNK1A1 G40N-P2A-Blast pLC-ZsGreen-P2A-Blast More ...
Expression of Long Noncoding RNA YIYA Promotes Glycolysis in Breast Cancer. Xing Z, Zhang Y, Liang K, Yan L, Xiang Y, Li C, Hu Q, Jin F, Putluri V, Putluri N, Coarfa C, Sreekumar A, Park PK, Nguyen TK, Wang S, Zhou J, Zhou Y, Marks JR, Hawke DH, Hung MC, Yang L, Han L, Ying H, Lin C. Cancer Res. 2018 Aug 15;78(16):4524-4532. doi: 10.1158/0008-5472.CAN-17-0385. Epub 2018 Jul 2. PubMed
DPP8/DPP9 inhibitor-induced pyroptosis for treatment of acute myeloid leukemia. Johnson DC, Taabazuing CY, Okondo MC, Chui AJ, Rao SD, Brown FC, Reed C, Peguero E, de Stanchina E, Kentsis A, Bachovchin DA. Nat Med. 2018 Aug;24(8):1151-1156. doi: 10.1038/s41591-018-0082-y. Epub 2018 Jul 2. PubMed
CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions. Zimmermann M, Murina O, Reijns MAM, Agathanggelou A, Challis R, Tarnauskaite Z, Muir M, Fluteau A, Aregger M, McEwan A, Yuan W, Clarke M, Lambros MB, Paneesha S, Moss P, Chandrashekhar M, Angers S, Moffat J, Brunton VG, Hart T, de Bono J, Stankovic T, Jackson AP, Durocher D. Nature. 2018 Jul;559(7713):285-289. doi: 10.1038/s41586-018-0291-z. Epub 2018 Jul 4. PubMed
Mutant ASXL1 cooperates with BAP1 to promote myeloid leukaemogenesis. Asada S, Goyama S, Inoue D, Shikata S, Takeda R, Fukushima T, Yonezawa T, Fujino T, Hayashi Y, Kawabata KC, Fukuyama T, Tanaka Y, Yokoyama A, Yamazaki S, Kozuka-Hata H, Oyama M, Kojima S, Kawazu M, Mano H, Kitamura T. Nat Commun. 2018 Jul 16;9(1):2733. doi: 10.1038/s41467-018-05085-9. PubMed
The shieldin complex mediates 53BP1-dependent DNA repair. Noordermeer SM, Adam S, Setiaputra D, Barazas M, Pettitt SJ, Ling AK, Olivieri M, Alvarez-Quilon A, Moatti N, Zimmermann M, Annunziato S, Krastev DB, Song F, Brandsma I, Frankum J, Brough R, Sherker A, Landry S, Szilard RK, Munro MM, McEwan A, Goullet de Rugy T, Lin ZY, Hart T, Moffat J, Gingras AC, Martin A, van Attikum H, Jonkers J, Lord CJ, Rottenberg S, Durocher D. Nature. 2018 Aug;560(7716):117-121. doi: 10.1038/s41586-018-0340-7. Epub 2018 Jul 18. PubMed Associated Plasmids pcDNA5-FRT/TO-FLAG-SHLD1 pCW-eGFP-SHLD1 pcDNA5-FRT/TO-FLAG-SHLD2 pGLUE-SHLD2 pCW-eGFP-SHLD2 More ...
Risk SNP-Mediated Promoter-Enhancer Switching Drives Prostate Cancer through lncRNA PCAT19. Hua JT, Ahmed M, Guo H, Zhang Y, Chen S, Soares F, Lu J, Zhou S, Wang M, Li H, Larson NB, McDonnell SK, Patel PS, Liang Y, Yao CQ, van der Kwast T, Lupien M, Feng FY, Zoubeidi A, Tsao MS, Thibodeau SN, Boutros PC, He HH. Cell. 2018 Jul 26;174(3):564-575.e18. doi: 10.1016/j.cell.2018.06.014. Epub 2018 Jul 19. PubMed
A novel enhancer regulates MGMT expression and promotes temozolomide resistance in glioblastoma. Chen X, Zhang M, Gan H, Wang H, Lee JH, Fang D, Kitange GJ, He L, Hu Z, Parney IF, Meyer FB, Giannini C, Sarkaria JN, Zhang Z. Nat Commun. 2018 Jul 27;9(1):2949. doi: 10.1038/s41467-018-05373-4. PubMed
Hippo signaling dysfunction induces cancer cell addiction to YAP. Han H, Yang B, Nakaoka HJ, Yang J, Zhao Y, Le Nguyen K, Bishara AT, Mandalia TK, Wang W. Oncogene. 2018 Dec;37(50):6414-6424. doi: 10.1038/s41388-018-0419-5. Epub 2018 Aug 1. PubMed
Reconstruction of the cell entry pathway of an extinct virus. Robinson-McCarthy LR, McCarthy KR, Raaben M, Piccinotti S, Nieuwenhuis J, Stubbs SH, Bakkers MJG, Whelan SPJ. PLoS Pathog. 2018 Aug 6;14(8):e1007123. doi: 10.1371/journal.ppat.1007123. eCollection 2018 Aug. PubMed
Nrf2 negatively regulates STING indicating a link between antiviral sensing and metabolic reprogramming. Olagnier D, Brandtoft AM, Gunderstofte C, Villadsen NL, Krapp C, Thielke AL, Laustsen A, Peri S, Hansen AL, Bonefeld L, Thyrsted J, Bruun V, Iversen MB, Lin L, Artegoitia VM, Su C, Yang L, Lin R, Balachandran S, Luo Y, Nyegaard M, Marrero B, Goldbach-Mansky R, Motwani M, Ryan DG, Fitzgerald KA, O'Neill LA, Hollensen AK, Damgaard CK, de Paoli FV, Bertram HC, Jakobsen MR, Poulsen TB, Holm CK. Nat Commun. 2018 Aug 29;9(1):3506. doi: 10.1038/s41467-018-05861-7. PubMed
Wnt Effector TCF4 Is Dispensable for Wnt Signaling in Human Cancer Cells. Hrckulak D, Janeckova L, Lanikova L, Kriz V, Horazna M, Babosova O, Vojtechova M, Galuskova K, Sloncova E, Korinek V. Genes (Basel). 2018 Sep 1;9(9). pii: genes9090439. doi: 10.3390/genes9090439. PubMed
PLK1 targets CtIP to promote microhomology-mediated end joining. Wang H, Qiu Z, Liu B, Wu Y, Ren J, Liu Y, Zhao Y, Wang Y, Hao S, Li Z, Peng B, Xu X. Nucleic Acids Res. 2018 Nov 16;46(20):10724-10739. doi: 10.1093/nar/gky810. PubMed
Transaminase Inhibition by 2-Hydroxyglutarate Impairs Glutamate Biosynthesis and Redox Homeostasis in Glioma. McBrayer SK, Mayers JR, DiNatale GJ, Shi DD, Khanal J, Chakraborty AA, Sarosiek KA, Briggs KJ, Robbins AK, Sewastianik T, Shareef SJ, Olenchock BA, Parker SJ, Tateishi K, Spinelli JB, Islam M, Haigis MC, Looper RE, Ligon KL, Bernstein BE, Carrasco RD, Cahill DP, Asara JM, Metallo CM, Yennawar NH, Vander Heiden MG, Kaelin WG Jr. Cell. 2018 Sep 20;175(1):101-116.e25. doi: 10.1016/j.cell.2018.08.038. Epub 2018 Sep 13. PubMed
Systematic genetic mapping of necroptosis identifies SLC39A7 as modulator of death receptor trafficking. Fauster A, Rebsamen M, Willmann KL, Cesar-Razquin A, Girardi E, Bigenzahn JW, Schischlik F, Scorzoni S, Bruckner M, Konecka J, Hormann K, Heinz LX, Boztug K, Superti-Furga G. Cell Death Differ. 2018 Sep 20. pii: 10.1038/s41418-018-0192-6. doi: 10.1038/s41418-018-0192-6. PubMed
Changes in mRNA abundance drive shuttling of RNA binding proteins, linking cytoplasmic RNA degradation to transcription. Gilbertson S, Federspiel JD, Hartenian E, Cristea IM, Glaunsinger B. Elife. 2018 Oct 3;7. pii: 37663. doi: 10.7554/eLife.37663. PubMed Associated Plasmids GFP-muSOX Thy1.1-muSOX Thy1.1-GFP Thy1.1-muSOX D219A
Compound C inhibits nonsense-mediated RNA decay independently of AMPK. Cheruiyot A, Li S, Nickless A, Roth R, Fitzpatrick JAJ, You Z. PLoS One. 2018 Oct 5;13(10):e0204978. doi: 10.1371/journal.pone.0204978. eCollection 2018. PubMed
Human DPP9 represses NLRP1 inflammasome and protects against autoinflammatory diseases via both peptidase activity and FIIND domain binding. Zhong FL, Robinson K, Teo DET, Tan KY, Lim C, Harapas CR, Yu CH, Xie WH, Sobota RM, Au VB, Hopkins R, D'Osualdo A, Reed JC, Connolly JE, Masters SL, Reversade B. J Biol Chem. 2018 Dec 7;293(49):18864-18878. doi: 10.1074/jbc.RA118.004350. Epub 2018 Oct 5. PubMed
Regulation of the Hippo Pathway by Phosphatidic Acid-Mediated Lipid-Protein Interaction. Han H, Qi R, Zhou JJ, Ta AP, Yang B, Nakaoka HJ, Seo G, Guan KL, Luo R, Wang W. Mol Cell. 2018 Oct 18;72(2):328-340.e8. doi: 10.1016/j.molcel.2018.08.038. Epub 2018 Oct 4. PubMed
Trichomonas vaginalis Induces NLRP3 Inflammasome Activation and Pyroptotic Cell Death in Human Macrophages. Riestra AM, Valderrama JA, Patras KA, Booth SD, Quek XY, Tsai CM, Nizet V. J Innate Immun. 2019;11(1):86-98. doi: 10.1159/000493585. Epub 2018 Nov 2. PubMed
Inhibition of Thioredoxin/Thioredoxin Reductase Induces Synthetic Lethality in Lung Cancers with Compromised Glutathione Homeostasis. Yan X, Zhang X, Wang L, Zhang R, Pu X, Wu S, Li L, Tong P, Wang J, Meng QH, Jensen VB, Girard L, Minna JD, Roth JA, Swisher SG, Heymach JV, Fang B. Cancer Res. 2019 Jan 1;79(1):125-132. doi: 10.1158/0008-5472.CAN-18-1938. Epub 2018 Nov 6. PubMed
PRC2 loss induces chemoresistance by repressing apoptosis in T cell acute lymphoblastic leukemia. Aries IM, Bodaar K, Karim SA, Chonghaile TN, Hinze L, Burns MA, Pfirrmann M, Degar J, Landrigan JT, Balbach S, Peirs S, Menten B, Isenhart R, Stevenson KE, Neuberg DS, Devidas M, Loh ML, Hunger SP, Teachey DT, Rabin KR, Winter SS, Dunsmore KP, Wood BL, Silverman LB, Sallan SE, Van Vlierberghe P, Orkin SH, Knoechel B, Letai AG, Gutierrez A. J Exp Med. 2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570. PubMed Associated Plasmids pLenti CMV Puro DEST (w118-1) IGF1R pLenti CMV Puro DEST (w118-1) MXRA7 pLenti CMV Puro DEST (w118-1) SDC3 pLenti CMV Puro DEST (w118-1) TSPAN9 pLenti CMV Puro DEST (w118-1) PHLDB1 More ...
DEP domain containing 1 predicts prognosis of hepatocellular carcinoma patients and regulates tumor proliferation and metastasis. Qu D, Cui F, Lu D, Yang Y, Xu Y. Cancer Sci. 2019 Jan;110(1):157-165. doi: 10.1111/cas.13867. Epub 2018 Dec 13. PubMed
Fibroblast Growth Factor 2 lethally sensitizes cancer cells to stress-targeted therapeutic inhibitors. Dias MH, Fonseca CS, Zeidler JD, Albuquerque LL, da Silva MS, Cararo-Lopes E, Reis MS, Noel V, Dos Santos EO, Prior IA, Armelin HA. Mol Oncol. 2019 Feb;13(2):290-306. doi: 10.1002/1878-0261.12402. Epub 2018 Dec 3. PubMed
Sigma-2 Receptor/TMEM97 and PGRMC-1 Increase the Rate of Internalization of LDL by LDL Receptor through the Formation of a Ternary Complex. Riad A, Zeng C, Weng CC, Winters H, Xu K, Makvandi M, Metz T, Carlin S, Mach RH. Sci Rep. 2018 Nov 15;8(1):16845. doi: 10.1038/s41598-018-35430-3. PubMed
Genome-wide CRISPR Screens in Primary Human T Cells Reveal Key Regulators of Immune Function. Shifrut E, Carnevale J, Tobin V, Roth TL, Woo JM, Bui CT, Li PJ, Diolaiti ME, Ashworth A, Marson A. Cell. 2018 Dec 13;175(7):1958-1971.e15. doi: 10.1016/j.cell.2018.10.024. Epub 2018 Nov 15. PubMed
DYNLL1 binds to MRE11 to limit DNA end resection in BRCA1-deficient cells. He YJ, Meghani K, Caron MC, Yang C, Ronato DA, Bian J, Sharma A, Moore J, Niraj J, Detappe A, Doench JG, Legube G, Root DE, D'Andrea AD, Drane P, De S, Konstantinopoulos PA, Masson JY, Chowdhury D. Nature. 2018 Nov;563(7732):522-526. doi: 10.1038/s41586-018-0670-5. Epub 2018 Oct 31. PubMed
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FOXA1 mutations alter pioneering activity, differentiation and prostate cancer phenotypes. Adams EJ, Karthaus WR, Hoover E, Liu D, Gruet A, Zhang Z, Cho H, DiLoreto R, Chhangawala S, Liu Y, Watson PA, Davicioni E, Sboner A, Barbieri CE, Bose R, Leslie CS, Sawyers CL. Nature. 2019 Jul;571(7765):408-412. doi: 10.1038/s41586-019-1318-9. Epub 2019 Jun 26. PubMed
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Genome-wide CRISPR screens reveal genetic mediators of cereblon modulator toxicity in primary effusion lymphoma. Patil A, Manzano M, Gottwein E. Blood Adv. 2019 Jul 23;3(14):2105-2117. doi: 10.1182/bloodadvances.2019031732. PubMed Associated Plasmids pLC-Flag-UBE2G1 sg2R-P2A-Hygro pLC-Flag-SENP8 sg2R-P2A-Hygro pLC-ZsGreen-P2A-Hygro pLC-ZsGreen-P2A-Puro pLC-Flag-CRBN-P2A-Hygro More ...
Network pharmacology modeling identifies synergistic Aurora B and ZAK interaction in triple-negative breast cancer. Tang J, Gautam P, Gupta A, He L, Timonen S, Akimov Y, Wang W, Szwajda A, Jaiswal A, Turei D, Yadav B, Kankainen M, Saarela J, Saez-Rodriguez J, Wennerberg K, Aittokallio T. NPJ Syst Biol Appl. 2019 Jul 8;5:20. doi: 10.1038/s41540-019-0098-z. eCollection 2019. PubMed
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Construction of a mavs-inactivated MDCK cell line for facilitating the propagation of canine distemper virus (CDV). Cai C, Wang X, Zhao Y, Yi C, Jin Z, Zhang A, Han L. Mol Immunol. 2019 Oct;114:133-138. doi: 10.1016/j.molimm.2019.06.013. Epub 2019 Jul 25. PubMed
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Fascin Controls Metastatic Colonization and Mitochondrial Oxidative Phosphorylation by Remodeling Mitochondrial Actin Filaments. Lin S, Huang C, Gunda V, Sun J, Chellappan SP, Li Z, Izumi V, Fang B, Koomen J, Singh PK, Hao J, Yang S. Cell Rep. 2019 Sep 10;28(11):2824-2836.e8. doi: 10.1016/j.celrep.2019.08.011. PubMed
Iron chelation and 2-oxoglutarate-dependent dioxygenase inhibition suppress mantle cell lymphoma's cyclin D1. Babosova O, Kapralova K, Raskova Kafkova L, Korinek V, Divoky V, Prchal JT, Lanikova L. J Cell Mol Med. 2019 Nov;23(11):7785-7795. doi: 10.1111/jcmm.14655. Epub 2019 Sep 13. PubMed
Optimized CRISPR guide RNA design for two high-fidelity Cas9 variants by deep learning. Wang D, Zhang C, Wang B, Li B, Wang Q, Liu D, Wang H, Zhou Y, Shi L, Lan F, Wang Y. Nat Commun. 2019 Sep 19;10(1):4284. doi: 10.1038/s41467-019-12281-8. PubMed
HIF-independent synthetic lethality between CDK4/6 inhibition and VHL loss across species. Nicholson HE, Tariq Z, Housden BE, Jennings RB, Stransky LA, Perrimon N, Signoretti S, Kaelin WG Jr. Sci Signal. 2019 Oct 1;12(601). pii: 12/601/eaay0482. doi: 10.1126/scisignal.aay0482. PubMed Associated Plasmids pLenti-EF1a-CDK6-mutPAM-PGK-puro pLX304-EV-IRES-GFP pLX304-VHL-IRES-GFP pLX304-VHLdeltaB-IRES-GFP pLX304-VHL-IRES-Tdtomato
Cell confinement reveals a branched-actin independent circuit for neutrophil polarity. Graziano BR, Town JP, Sitarska E, Nagy TL, Fosnaric M, Penic S, Iglic A, Kralj-Iglic V, Gov NS, Diz-Munoz A, Weiner OD. PLoS Biol. 2019 Oct 10;17(10):e3000457. doi: 10.1371/journal.pbio.3000457. eCollection 2019 Oct. PubMed
A one-step tRNA-CRISPR system for genome-wide genetic interaction mapping in mammalian cells. Zhao Y, Tyrishkin K, Sjaarda C, Khanal P, Stafford J, Rauh M, Liu X, Babak T, Yang X. Sci Rep. 2019 Oct 10;9(1):14499. doi: 10.1038/s41598-019-51090-3. PubMed
Mutational Analysis of Sigma-1 Receptor's Role in Synaptic Stability. Ryskamp DA, Zhemkov V, Bezprozvanny I. Front Neurosci. 2019 Sep 19;13:1012. doi: 10.3389/fnins.2019.01012. eCollection 2019. PubMed
Genome-wide CRISPR/Cas9 library screening identified PHGDH as a critical driver for Sorafenib resistance in HCC. Wei L, Lee D, Law CT, Zhang MS, Shen J, Chin DW, Zhang A, Tsang FH, Wong CL, Ng IO, Wong CC, Wong CM. Nat Commun. 2019 Oct 15;10(1):4681. doi: 10.1038/s41467-019-12606-7. PubMed
The ADP/ATP translocase drives mitophagy independent of nucleotide exchange. Hoshino A, Wang WJ, Wada S, McDermott-Roe C, Evans CS, Gosis B, Morley MP, Rathi KS, Li J, Li K, Yang S, McManus MJ, Bowman C, Potluri P, Levin M, Damrauer S, Wallace DC, Holzbaur ELF, Arany Z. Nature. 2019 Nov;575(7782):375-379. doi: 10.1038/s41586-019-1667-4. Epub 2019 Oct 16. PubMed
Nuclear receptors regulate alternative lengthening of telomeres through a novel noncanonical FANCD2 pathway. Xu M, Qin J, Wang L, Lee HJ, Kao CY, Liu D, Songyang Z, Chen J, Tsai MJ, Tsai SY. Sci Adv. 2019 Oct 9;5(10):eaax6366. doi: 10.1126/sciadv.aax6366. eCollection 2019 Oct. PubMed
Search-and-replace genome editing without double-strand breaks or donor DNA. Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, Chen PJ, Wilson C, Newby GA, Raguram A, Liu DR. Nature. 2019 Oct 21. pii: 10.1038/s41586-019-1711-4. doi: 10.1038/s41586-019-1711-4. PubMed Associated Plasmids pCMV-PE1 pCMV-PE2 pCMV-PE2-P2A-GFP pU6-pegRNA-GG-acceptor pU6-Sp-pegRNA-HEK3_CTT_ins More ...
PIK3CA Cooperates with KRAS to Promote MYC Activity and Tumorigenesis via the Bromodomain Protein BRD9. Bell CM, Raffeiner P, Hart JR, Vogt PK. Cancers (Basel). 2019 Oct 24;11(11). pii: cancers11111634. doi: 10.3390/cancers11111634. PubMed
Antitumor immunity augments the therapeutic effects of p53 activation on acute myeloid leukemia. Hayashi Y, Goyama S, Liu X, Tamura M, Asada S, Tanaka Y, Fukuyama T, Wunderlich M, O'Brien E, Mizukawa B, Yamazaki S, Matsumoto A, Yamasaki S, Shibata T, Matsuda K, Sashida G, Takizawa H, Kitamura T. Nat Commun. 2019 Oct 25;10(1):4869. doi: 10.1038/s41467-019-12555-1. PubMed
Cholesterol accessibility at the ciliary membrane controls hedgehog signaling. Kinnebrew M, Iverson EJ, Patel BB, Pusapati GV, Kong JH, Johnson KA, Luchetti G, Eckert KM, McDonald JG, Covey DF, Siebold C, Radhakrishnan A, Rohatgi R. Elife. 2019 Oct 30;8. pii: 50051. doi: 10.7554/eLife.50051. PubMed
Biological plasticity rescues target activity in CRISPR knock outs. Smits AH, Ziebell F, Joberty G, Zinn N, Mueller WF, Clauder-Munster S, Eberhard D, Falth Savitski M, Grandi P, Jakob P, Michon AM, Sun H, Tessmer K, Burckstummer T, Bantscheff M, Steinmetz LM, Drewes G, Huber W. Nat Methods. 2019 Nov;16(11):1087-1093. doi: 10.1038/s41592-019-0614-5. Epub 2019 Oct 28. PubMed
Inducible Exoc7/Exo70 knockout reveals a critical role of the exocyst in insulin-regulated GLUT4 exocytosis. Wang S, Crisman L, Miller J, Datta I, Gulbranson DR, Tian Y, Yin Q, Yu H, Shen J. J Biol Chem. 2019 Dec 27;294(52):19988-19996. doi: 10.1074/jbc.RA119.010821. Epub 2019 Nov 18. PubMed Associated Plasmids SHC003BSD-EXOC7 SHC003BSD SHC003BSD-DelGFP TLCV2-DelGFP TLCV2-Exoc7-Ex5&10
GPR146 Deficiency Protects against Hypercholesterolemia and Atherosclerosis. Yu H, Rimbert A, Palmer AE, Toyohara T, Xia Y, Xia F, Ferreira LMR, Chen Z, Chen T, Loaiza N, Horwitz NB, Kacergis MC, Zhao L, Soukas AA, Kuivenhoven JA, Kathiresan S, Cowan CA. Cell. 2019 Nov 27;179(6):1276-1288.e14. doi: 10.1016/j.cell.2019.10.034. PubMed
Elucidation of WW domain ligand binding specificities in the Hippo pathway reveals STXBP4 as YAP inhibitor. Vargas RE, Duong VT, Han H, Ta AP, Chen Y, Zhao S, Yang B, Seo G, Chuc K, Oh S, El Ali A, Razorenova OV, Chen J, Luo R, Li X, Wang W. EMBO J. 2020 Jan 2;39(1):e102406. doi: 10.15252/embj.2019102406. Epub 2019 Nov 29. PubMed
Genome-wide CRISPR screen identifies ELP5 as a determinant of gemcitabine sensitivity in gallbladder cancer. Xu S, Zhan M, Jiang C, He M, Yang L, Shen H, Huang S, Huang X, Lin R, Shi Y, Liu Q, Chen W, Mohan M, Wang J. Nat Commun. 2019 Dec 2;10(1):5492. doi: 10.1038/s41467-019-13420-x. PubMed
Somatic inflammatory gene mutations in human ulcerative colitis epithelium. Nanki K, Fujii M, Shimokawa M, Matano M, Nishikori S, Date S, Takano A, Toshimitsu K, Ohta Y, Takahashi S, Sugimoto S, Ishimaru K, Kawasaki K, Nagai Y, Ishii R, Yoshida K, Sasaki N, Hibi T, Ishihara S, Kanai T, Sato T. Nature. 2020 Jan;577(7789):254-259. doi: 10.1038/s41586-019-1844-5. Epub 2019 Dec 18. PubMed
PrP is a central player in toxicity mediated by soluble aggregates of neurodegeneration-causing proteins. Corbett GT, Wang Z, Hong W, Colom-Cadena M, Rose J, Liao M, Asfaw A, Hall TC, Ding L, DeSousa A, Frosch MP, Collinge J, Harris DA, Perkinton MS, Spires-Jones TL, Young-Pearse TL, Billinton A, Walsh DM. Acta Neuropathol. 2020 Mar;139(3):503-526. doi: 10.1007/s00401-019-02114-9. Epub 2019 Dec 18. PubMed
LINC02273 drives breast cancer metastasis by epigenetically increasing AGR2 transcription. Xiu B, Chi Y, Liu L, Chi W, Zhang Q, Chen J, Guo R, Si J, Li L, Xue J, Shao ZM, Wu ZH, Huang S, Wu J. Mol Cancer. 2019 Dec 19;18(1):187. doi: 10.1186/s12943-019-1115-y. PubMed
WBP11 is required for splicing the TUBGCP6 pre-mRNA to promote centriole duplication. Park EM, Scott PM, Clutario K, Cassidy KB, Zhan K, Gerber SA, Holland AJ. J Cell Biol. 2020 Jan 6;219(1). pii: 133543. doi: 10.1083/jcb.201904203. PubMed
RNA isoform screens uncover the essentiality and tumor-suppressor activity of ultraconserved poison exons. Thomas JD, Polaski JT, Feng Q, De Neef EJ, Hoppe ER, McSharry MV, Pangallo J, Gabel AM, Belleville AE, Watson J, Nkinsi NT, Berger AH, Bradley RK. Nat Genet. 2020 Jan;52(1):84-94. doi: 10.1038/s41588-019-0555-z. Epub 2020 Jan 7. PubMed Associated Plasmids Human CRISPR Poison Exon Knockout Library
IQGAP1 restrains T-cell cosignaling mediated by OX40. Okuyama Y, Nagashima H, Ushio-Fukai M, Croft M, Ishii N, So T. FASEB J. 2020 Jan;34(1):540-554. doi: 10.1096/fj.201900879RR. Epub 2019 Nov 25. PubMed
Rapid non-uniform adaptation to conformation-specific KRAS(G12C) inhibition. Xue JY, Zhao Y, Aronowitz J, Mai TT, Vides A, Qeriqi B, Kim D, Li C, de Stanchina E, Mazutis L, Risso D, Lito P. Nature. 2020 Jan;577(7790):421-425. doi: 10.1038/s41586-019-1884-x. Epub 2020 Jan 8. PubMed
Genome-wide CRISPR screen identifies host dependency factors for influenza A virus infection. Li B, Clohisey SM, Chia BS, Wang B, Cui A, Eisenhaure T, Schweitzer LD, Hoover P, Parkinson NJ, Nachshon A, Smith N, Regan T, Farr D, Gutmann MU, Bukhari SI, Law A, Sangesland M, Gat-Viks I, Digard P, Vasudevan S, Lingwood D, Dockrell DH, Doench JG, Baillie JK, Hacohen N. Nat Commun. 2020 Jan 9;11(1):164. doi: 10.1038/s41467-019-13965-x. PubMed
Genome-Wide CRISPR Screen Reveals Cancer Cell Resistance to NK Cells Induced by NK-Derived IFN-gamma. Zhuang X, Veltri DP, Long EO. Front Immunol. 2019 Dec 11;10:2879. doi: 10.3389/fimmu.2019.02879. eCollection 2019. PubMed
Diverse AR Gene Rearrangements Mediate Resistance to Androgen Receptor Inhibitors in Metastatic Prostate Cancer. Li Y, Yang R, Henzler CM, Ho Y, Passow C, Auch B, Carreira S, Nava Rodrigues D, Bertan C, Hwang TH, Quigley DA, Dang HX, Morrissey C, Fraser M, Plymate SR, Maher CA, Feng FY, de Bono JS, Dehm SM. Clin Cancer Res. 2020 Apr 15;26(8):1965-1976. doi: 10.1158/1078-0432.CCR-19-3023. Epub 2020 Jan 13. PubMed
Epigenetic Silencing of CDR1as Drives IGF2BP3-Mediated Melanoma Invasion and Metastasis. Hanniford D, Ulloa-Morales A, Karz A, Berzoti-Coelho MG, Moubarak RS, Sanchez-Sendra B, Kloetgen A, Davalos V, Imig J, Wu P, Vasudevaraja V, Argibay D, Lilja K, Tabaglio T, Monteagudo C, Guccione E, Tsirigos A, Osman I, Aifantis I, Hernando E. Cancer Cell. 2020 Jan 13;37(1):55-70.e15. doi: 10.1016/j.ccell.2019.12.007. PubMed
Dynamic post-translational modification profiling of Mycobacterium tuberculosis-infected primary macrophages. Budzik JM, Swaney DL, Jimenez-Morales D, Johnson JR, Garelis NE, Repasy T, Roberts AW, Popov LM, Parry TJ, Pratt D, Ideker T, Krogan NJ, Cox JS. Elife. 2020 Jan 17;9. pii: 51461. doi: 10.7554/eLife.51461. PubMed
Extracellular NAD(+) enhances PARP-dependent DNA repair capacity independently of CD73 activity. Wilk A, Hayat F, Cunningham R, Li J, Garavaglia S, Zamani L, Ferraris DM, Sykora P, Andrews J, Clark J, Davis A, Chaloin L, Rizzi M, Migaud M, Sobol RW. Sci Rep. 2020 Jan 20;10(1):651. doi: 10.1038/s41598-020-57506-9. PubMed Associated Plasmids pLentiGuide-Puro/CD73-gRNA4 pLV-CMV-hXRCC1-mCherry-Hygro
IL-4 controls activated neutrophil FcgammaR2b expression and migration into inflamed joints. Panda SK, Wigerblad G, Jiang L, Jimenez-Andrade Y, Iyer VS, Shen Y, Boddul SV, Guerreiro-Cacais AO, Raposo B, Kasza Z, Wermeling F. Proc Natl Acad Sci U S A. 2020 Jan 24. pii: 1914186117. doi: 10.1073/pnas.1914186117. PubMed Associated Plasmids LentiGuide Puro-P2A-EGFP LentiGuide-Puro-P2A-EGFP_mRFPstuf
Interrogation of enhancer function by enhancer-targeting CRISPR epigenetic editing. Li K, Liu Y, Cao H, Zhang Y, Gu Z, Liu X, Yu A, Kaphle P, Dickerson KE, Ni M, Xu J. Nat Commun. 2020 Jan 24;11(1):485. doi: 10.1038/s41467-020-14362-5. PubMed Associated Plasmids pHR_TRE3G-p300-dCas9-P2A-mCherry Lenti_MCP-LSD1_Hygro Lenti_MCP-VP64_Hygro Lenti_MCP-p300_Hygro Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1 More ...
Cooperation of the NEIL3 and Fanconi anemia/BRCA pathways in interstrand crosslink repair. Li N, Wang J, Wallace SS, Chen J, Zhou J, D'Andrea AD. Nucleic Acids Res. 2020 Apr 6;48(6):3014-3028. doi: 10.1093/nar/gkaa038. PubMed
The mechanism of how CD95/Fas activates the Type I IFN/STAT1 axis, driving cancer stemness in breast cancer. Qadir AS, Stults AM, Murmann AE, Peter ME. Sci Rep. 2020 Jan 28;10(1):1310. doi: 10.1038/s41598-020-58211-3. PubMed
The ARK Assay Is a Sensitive and Versatile Method for the Global Detection of DNA-Protein Crosslinks. Hu Q, Klages-Mundt N, Wang R, Lynn E, Kuma Saha L, Zhang H, Srivastava M, Shen X, Tian Y, Kim H, Ye Y, Paull T, Takeda S, Chen J, Li L. Cell Rep. 2020 Jan 28;30(4):1235-1245.e4. doi: 10.1016/j.celrep.2019.12.067. PubMed
Identification of functional regulatory elements in the human genome using pooled CRISPR screens. Borys SM, Younger ST. BMC Genomics. 2020 Jan 31;21(1):107. doi: 10.1186/s12864-020-6497-0. PubMed
Cancer Cell-Derived Matrisome Proteins Promote Metastasis in Pancreatic Ductal Adenocarcinoma. Tian C, Ohlund D, Rickelt S, Lidstrom T, Huang Y, Hao L, Zhao RT, Franklin O, Bhatia SN, Tuveson DA, Hynes RO. Cancer Res. 2020 Apr 1;80(7):1461-1474. doi: 10.1158/0008-5472.CAN-19-2578. Epub 2020 Feb 6. PubMed
A combination strategy targeting enhancer plasticity exerts synergistic lethality against BETi-resistant leukemia cells. Guo L, Li J, Zeng H, Guzman AG, Li T, Lee M, Zhou Y, Goodell MA, Stephan C, Davies PJA, Dawson MA, Sun D, Huang Y. Nat Commun. 2020 Feb 6;11(1):740. doi: 10.1038/s41467-020-14604-6. PubMed
Cell fitness screens reveal a conflict between LINE-1 retrotransposition and DNA replication. Ardeljan D, Steranka JP, Liu C, Li Z, Taylor MS, Payer LM, Gorbounov M, Sarnecki JS, Deshpande V, Hruban RH, Boeke JD, Fenyo D, Wu PH, Smogorzewska A, Holland AJ, Burns KH. Nat Struct Mol Biol. 2020 Feb;27(2):168-178. doi: 10.1038/s41594-020-0372-1. Epub 2020 Feb 10. PubMed Associated Plasmids pDA007 pDA025 pDA027 pDA034 pDA056 More ...
Glycerol-3-phosphate is an FGF23 regulator derived from the injured kidney. Simic P, Kim W, Zhou W, Pierce KA, Chang W, Sykes DB, Aziz NB, Elmariah S, Ngo D, Pajevic PD, Govea N, Kestenbaum BR, de Boer IH, Cheng Z, Christov M, Chun J, Leaf DE, Waikar SS, Tager AM, Gerszten RE, Thadhani RI, Clish CB, Juppner H, Wein MN, Rhee EP. J Clin Invest. 2020 Mar 2;130(3):1513-1526. doi: 10.1172/JCI131190. PubMed
Local rewiring of genome-nuclear lamina interactions by transcription. Brueckner L, Zhao PA, van Schaik T, Leemans C, Sima J, Peric-Hupkes D, Gilbert DM, van Steensel B. EMBO J. 2020 Mar 16;39(6):e103159. doi: 10.15252/embj.2019103159. Epub 2020 Feb 21. PubMed
Systematic analysis of the Hippo pathway organization and oncogenic alteration in evolution. Chen Y, Han H, Seo G, Vargas RE, Yang B, Chuc K, Zhao H, Wang W. Sci Rep. 2020 Feb 21;10(1):3173. doi: 10.1038/s41598-020-60120-4. PubMed
Protein Translocation Acquires Substrate Selectivity Through ER Stress-Induced Reassembly of Translocon Auxiliary Components. Lee S, Shin Y, Kim K, Song Y, Kim Y, Kang SW. Cells. 2020 Feb 24;9(2). pii: cells9020518. doi: 10.3390/cells9020518. PubMed
A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA. de Jong OG, Murphy DE, Mager I, Willms E, Garcia-Guerra A, Gitz-Francois JJ, Lefferts J, Gupta D, Steenbeek SC, van Rheenen J, El Andaloussi S, Schiffelers RM, Wood MJA, Vader P. Nat Commun. 2020 Feb 28;11(1):1113. doi: 10.1038/s41467-020-14977-8. PubMed
Acetylation of MORC2 by NAT10 regulates cell-cycle checkpoint control and resistance to DNA-damaging chemotherapy and radiotherapy in breast cancer. Liu HY, Liu YY, Yang F, Zhang L, Zhang FL, Hu X, Shao ZM, Li DQ. Nucleic Acids Res. 2020 Apr 17;48(7):3638-3656. doi: 10.1093/nar/gkaa130. PubMed
Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5'G-extended sgRNAs. Kulcsar PI, Talas A, Toth E, Nyeste A, Ligeti Z, Welker Z, Welker E. Nat Commun. 2020 Mar 6;11(1):1223. doi: 10.1038/s41467-020-15021-5. PubMed Associated Plasmids pX330-Flag-WT_SpCas9 (without sgRNA; with silent mutations) pX330-Flag-eSpCas9 (without sgRNA; with silent mutations) pX330-Flag-SpCas9-HF1 (without sgRNA; with silent mutations) pX330-Flag-HypaSpCas9 (without sgRNA; with silent mutations) pX330-Flag-evoSpCas9 (without sgRNA; with silent mutations) More ...
CRISPR/Cas9-targeting of CD40 in hematopoietic stem cells limits immune activation mediated by anti-CD40. Wang R, Graham S, Sun N, McCarthy D, Peng R, Erickson J, Oconnor L, Zhu X, Wurbel M, Dunstan R, Westmoreland S, Chung N, Ghayur T, Gu J. PLoS One. 2020 Mar 10;15(3):e0228221. doi: 10.1371/journal.pone.0228221. eCollection 2020. PubMed
Transgenesis and Genome Editing of Mouse Spermatogonial Stem Cells by Lentivirus Pseudotyped with Sendai Virus F Protein. Shinohara T, Kanatsu-Shinohara M. Stem Cell Reports. 2020 Mar 10;14(3):447-461. doi: 10.1016/j.stemcr.2020.02.001. PubMed
Genetic Manipulation of Human Intestinal Enteroids Demonstrates the Necessity of a Functional Fucosyltransferase 2 Gene for Secretor-Dependent Human Norovirus Infection. Haga K, Ettayebi K, Tenge VR, Karandikar UC, Lewis MA, Lin SC, Neill FH, Ayyar BV, Zeng XL, Larson G, Ramani S, Atmar RL, Estes MK. mBio. 2020 Mar 17;11(2). pii: mBio.00251-20. doi: 10.1128/mBio.00251-20. PubMed
CRISPR-Cas9 Dual-gRNA Attack Causes Mutation, Excision and Inversion of the HIV-1 Proviral DNA. Binda CS, Klaver B, Berkhout B, Das AT. Viruses. 2020 Mar 18;12(3). pii: v12030330. doi: 10.3390/v12030330. PubMed
CD20 is dispensable for B-cell receptor signaling but is required for proper actin polymerization, adhesion and migration of malignant B cells. Kozlova V, Ledererova A, Ladungova A, Peschelova H, Janovska P, Slusarczyk A, Domagala J, Kopcil P, Vakulova V, Oppelt J, Bryja V, Doubek M, Mayer J, Pospisilova S, Smida M. PLoS One. 2020 Mar 25;15(3):e0229170. doi: 10.1371/journal.pone.0229170. eCollection 2020. PubMed
Heparan sulfate is a clearance receptor for aberrant extracellular proteins. Itakura E, Chiba M, Murata T, Matsuura A. J Cell Biol. 2020 Mar 2;219(3). pii: 133807. doi: 10.1083/jcb.201911126. PubMed
Targeting clinical epigenetic reprogramming for chemoprevention of metabolic and viral hepatocellular carcinoma. Juhling F, Hamdane N, Crouchet E, Li S, El Saghire H, Mukherji A, Fujiwara N, Oudot MA, Thumann C, Saviano A, Roca Suarez AA, Goto K, Masia R, Sojoodi M, Arora G, Aikata H, Ono A, Tabrizian P, Schwartz M, Polyak SJ, Davidson I, Schmidl C, Bock C, Schuster C, Chayama K, Pessaux P, Tanabe KK, Hoshida Y, Zeisel MB, Duong FH, Fuchs BC, Baumert TF. Gut. 2021 Jan;70(1):157-169. doi: 10.1136/gutjnl-2019-318918. Epub 2020 Mar 26. PubMed
Performance of anti-CD19 chimeric antigen receptor T cells in genetically defined classes of chronic lymphocytic leukemia. Mancikova V, Peschelova H, Kozlova V, Ledererova A, Ladungova A, Verner J, Loja T, Folber F, Mayer J, Pospisilova S, Smida M. J Immunother Cancer. 2020 Mar;8(1). pii: jitc-2019-000471. doi: 10.1136/jitc-2019-000471. PubMed
CD21 (Complement Receptor 2) Is the Receptor for Epstein-Barr Virus Entry into T Cells. Smith NA, Coleman CB, Gewurz BE, Rochford R. J Virol. 2020 May 18;94(11). pii: JVI.00428-20. doi: 10.1128/JVI.00428-20. Print 2020 May 18. PubMed
Loss of N-Glycanase 1 Alters Transcriptional and Translational Regulation in K562 Cell Lines. Mueller WF, Jakob P, Sun H, Clauder-Munster S, Ghidelli-Disse S, Ordonez D, Boesche M, Bantscheff M, Collier P, Haase B, Benes V, Paulsen M, Sehr P, Lewis J, Drewes G, Steinmetz LM. G3 (Bethesda). 2020 May 4;10(5):1585-1597. doi: 10.1534/g3.119.401031. PubMed
Genome-Wide CRISPR Screen Identifies Semaphorin 6A and 6B as Receptors for Paeniclostridium sordellii Toxin TcsL. Tian S, Liu Y, Wu H, Liu H, Zeng J, Choi MY, Chen H, Gerhard R, Dong M. Cell Host Microbe. 2020 May 13;27(5):782-792.e7. doi: 10.1016/j.chom.2020.03.007. Epub 2020 Apr 16. PubMed
Endocrine-Exocrine Signaling Drives Obesity-Associated Pancreatic Ductal Adenocarcinoma. Chung KM, Singh J, Lawres L, Dorans KJ, Garcia C, Burkhardt DB, Robbins R, Bhutkar A, Cardone R, Zhao X, Babic A, Vayrynen SA, Dias Costa A, Nowak JA, Chang DT, Dunne RF, Hezel AF, Koong AC, Wilhelm JJ, Bellin MD, Nylander V, Gloyn AL, McCarthy MI, Kibbey RG, Krishnaswamy S, Wolpin BM, Jacks T, Fuchs CS, Muzumdar MD. Cell. 2020 May 14;181(4):832-847.e18. doi: 10.1016/j.cell.2020.03.062. Epub 2020 Apr 17. PubMed
Distinct roles of BRCA2 in replication fork protection in response to hydroxyurea and DNA interstrand cross-links. Rickman KA, Noonan RJ, Lach FP, Sridhar S, Wang AT, Abhyankar A, Huang A, Kelly M, Auerbach AD, Smogorzewska A. Genes Dev. 2020 Jun 1;34(11-12):832-846. doi: 10.1101/gad.336446.120. Epub 2020 Apr 30. PubMed
Hdac3 is an epigenetic inhibitor of the cytotoxicity program in CD8 T cells. Tay RE, Olawoyin O, Cejas P, Xie Y, Meyer CA, Weng QY, Fisher DE, Long HW, Brown M, Kim HJ, Wucherpfennig KW. J Exp Med. 2020 Jul 6;217(7). pii: 151741. doi: 10.1084/jem.20191453. PubMed
Biochemical characteristics of the chondrocyte-enriched SNORC protein and its transcriptional regulation by SOX9. Jaiswal PK, Aljebali L, Gaumond MH, Oh CD, Yasuda H, Moffatt P. Sci Rep. 2020 May 8;10(1):7790. doi: 10.1038/s41598-020-64640-x. PubMed
LINC00261 Is Differentially Expressed in Pancreatic Cancer Subtypes and Regulates a Pro-Epithelial Cell Identity. Dorn A, Glass M, Neu CT, Heydel B, Huttelmaier S, Gutschner T, Haemmerle M. Cancers (Basel). 2020 May 13;12(5). pii: cancers12051227. doi: 10.3390/cancers12051227. PubMed
Direct Antiviral Activity of IFN-Stimulated Genes Is Responsible for Resistance to Paramyxoviruses in ISG15-Deficient Cells. Holthaus D, Vasou A, Bamford CGG, Andrejeva J, Paulus C, Randall RE, McLauchlan J, Hughes DJ. J Immunol. 2020 Jul 1;205(1):261-271. doi: 10.4049/jimmunol.1901472. Epub 2020 May 18. PubMed
Sense-antisense miRNA pairs constitute an elaborate reciprocal regulatory circuit. Song Y, Li L, Yang W, Fu Q, Chen W, Fang Z, Li W, Gu N, Zhang R. Genome Res. 2020 May;30(5):661-672. doi: 10.1101/gr.257121.119. Epub 2020 May 18. PubMed
DNA methylation enzymes and PRC1 restrict B-cell Epstein-Barr virus oncoprotein expression. Guo R, Zhang Y, Teng M, Jiang C, Schineller M, Zhao B, Doench JG, O'Reilly RJ, Cesarman E, Giulino-Roth L, Gewurz BE. Nat Microbiol. 2020 Aug;5(8):1051-1063. doi: 10.1038/s41564-020-0724-y. Epub 2020 May 18. PubMed
Loss of heterozygosity of essential genes represents a widespread class of potential cancer vulnerabilities. Nichols CA, Gibson WJ, Brown MS, Kosmicki JA, Busanovich JP, Wei H, Urbanski LM, Curimjee N, Berger AC, Gao GF, Cherniack AD, Dhe-Paganon S, Paolella BR, Beroukhim R. Nat Commun. 2020 May 20;11(1):2517. doi: 10.1038/s41467-020-16399-y. PubMed
Mitochondrial phosphatase PGAM5 modulates cellular senescence by regulating mitochondrial dynamics. Yu B, Ma J, Li J, Wang D, Wang Z, Wang S. Nat Commun. 2020 May 21;11(1):2549. doi: 10.1038/s41467-020-16312-7. PubMed
Targeting E. coli invasion of the blood-brain barrier for investigating the pathogenesis and therapeutic development of E. coli meningitis. Zhu N, Liu W, Prakash A, Zhang C, Kim KS. Cell Microbiol. 2020 May 24:e13231. doi: 10.1111/cmi.13231. PubMed
HRP2-DPF3a-BAF complex coordinates histone modification and chromatin remodeling to regulate myogenic gene transcription. Zhu X, Lan B, Yi X, He C, Dang L, Zhou X, Lu Y, Sun Y, Liu Z, Bai X, Zhang K, Li B, Li MJ, Chen Y, Zhang L. Nucleic Acids Res. 2020 Jul 9;48(12):6563-6582. doi: 10.1093/nar/gkaa441. PubMed
CRISPR screen in regulatory T cells reveals modulators of Foxp3. Cortez JT, Montauti E, Shifrut E, Gatchalian J, Zhang Y, Shaked O, Xu Y, Roth TL, Simeonov DR, Zhang Y, Chen S, Li Z, Woo JM, Ho J, Vogel IA, Prator GY, Zhang B, Lee Y, Sun Z, Ifergan I, Van Gool F, Hargreaves DC, Bluestone JA, Marson A, Fang D. Nature. 2020 Jun;582(7812):416-420. doi: 10.1038/s41586-020-2246-4. Epub 2020 Apr 29. PubMed
LRRK2 Kinase Inhibition Rescues Deficits in Lysosome Function Due to Heterozygous GBA1 Expression in Human iPSC-Derived Neurons. Sanyal A, Novis HS, Gasser E, Lin S, LaVoie MJ. Front Neurosci. 2020 May 15;14:442. doi: 10.3389/fnins.2020.00442. eCollection 2020. PubMed
Tumor Interferon Signaling Is Regulated by a lncRNA INCR1 Transcribed from the PD-L1 Locus. Mineo M, Lyons SM, Zdioruk M, von Spreckelsen N, Ferrer-Luna R, Ito H, Alayo QA, Kharel P, Giantini Larsen A, Fan WY, Auduong S, Grauwet K, Passaro C, Khalsa JK, Shah K, Reardon DA, Ligon KL, Beroukhim R, Nakashima H, Ivanov P, Anderson PJ, Lawler SE, Chiocca EA. Mol Cell. 2020 Jun 18;78(6):1207-1223.e8. doi: 10.1016/j.molcel.2020.05.015. Epub 2020 Jun 5. PubMed
Direct-seq: programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen. Song Q, Ni K, Liu M, Li Y, Wang L, Wang Y, Liu Y, Yu Z, Qi Y, Lu Z, Ma L. Genome Biol. 2020 Jun 8;21(1):136. doi: 10.1186/s13059-020-02044-w. PubMed Associated Plasmids Direct-seq_lentiGuide-Puro-Tail-8A8G Direct-seq_lentiGuide-Puro-Tetra Loop-8A8G Direct-seq_lentiGuide-Puro-Loop2-8A8G Direct-seq_lentiGuide-Puro-tRNA-Tail-8A8G Direct-seq_lentiGuide-Puro-tRNA-Tetra Loop-8A8G More ...
Massively parallel Cas13 screens reveal principles for guide RNA design. Wessels HH, Mendez-Mancilla A, Guo X, Legut M, Daniloski Z, Sanjana NE. Nat Biotechnol. 2020 Jun;38(6):722-727. doi: 10.1038/s41587-020-0456-9. Epub 2020 Mar 16. PubMed Associated Plasmids pLentiRNACRISPR_001 - hU6-DR_BsmBI-EFS-PguCas13b-NLS-2A-Puro-WPRE pLentiRNACRISPR_002 - hU6-DR_BsmBI-EFS-PguCas13b-NES-2A-Puro-WPRE pLentiRNACRISPR_003 - hU6-DR_BsmBI-EFS-PspCas13b-NLS-2A-Puro-WPRE pLentiRNACRISPR_004 - hU6-DR_BsmBI-EFS-PspCas13b-NES-2A-Puro-WPRE pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE More ...
A Genome-Wide CRISPR-Cas9 Screen Reveals the Requirement of Host Cell Sulfation for Schmallenberg Virus Infection. Thamamongood T, Aebischer A, Wagner V, Chang MW, Elling R, Benner C, Garcia-Sastre A, Kochs G, Beer M, Schwemmle M. J Virol. 2020 Aug 17;94(17). pii: JVI.00752-20. doi: 10.1128/JVI.00752-20. Print 2020 Aug 17. PubMed
The Tumour Suppressor TMEM127 Is a Nedd4-Family E3 Ligase Adaptor Required by Salmonella SteD to Ubiquitinate and Degrade MHC Class II Molecules. Alix E, Godlee C, Cerny O, Blundell S, Tocci R, Matthews S, Liu M, Pruneda JN, Swatek KN, Komander D, Sleap T, Holden DW. Cell Host Microbe. 2020 Jul 8;28(1):54-68.e7. doi: 10.1016/j.chom.2020.04.024. Epub 2020 Jun 10. PubMed
Targeting FTO Suppresses Cancer Stem Cell Maintenance and Immune Evasion. Su R, Dong L, Li Y, Gao M, Han L, Wunderlich M, Deng X, Li H, Huang Y, Gao L, Li C, Zhao Z, Robinson S, Tan B, Qing Y, Qin X, Prince E, Xie J, Qin H, Li W, Shen C, Sun J, Kulkarni P, Weng H, Huang H, Chen Z, Zhang B, Wu X, Olsen MJ, Muschen M, Marcucci G, Salgia R, Li L, Fathi AT, Li Z, Mulloy JC, Wei M, Horne D, Chen J. Cancer Cell. 2020 Jul 13;38(1):79-96.e11. doi: 10.1016/j.ccell.2020.04.017. Epub 2020 Jun 11. PubMed
Repurposing type I-F CRISPR-Cas system as a transcriptional activation tool in human cells. Chen Y, Liu J, Zhi S, Zheng Q, Ma W, Huang J, Liu Y, Liu D, Liang P, Songyang Z. Nat Commun. 2020 Jun 19;11(1):3136. doi: 10.1038/s41467-020-16880-8. PubMed Associated Plasmids pCsy1-Csy2 pCsy3-VPR-Csy4 pCsy-crRNA-EV
MAP4K Interactome Reveals STRN4 as a Key STRIPAK Complex Component in Hippo Pathway Regulation. Seo G, Han H, Vargas RE, Yang B, Li X, Wang W. Cell Rep. 2020 Jul 7;32(1):107860. doi: 10.1016/j.celrep.2020.107860. PubMed
A Genetic Map of the Response to DNA Damage in Human Cells. Olivieri M, Cho T, Alvarez-Quilon A, Li K, Schellenberg MJ, Zimmermann M, Hustedt N, Rossi SE, Adam S, Melo H, Heijink AM, Sastre-Moreno G, Moatti N, Szilard RK, McEwan A, Ling AK, Serrano-Benitez A, Ubhi T, Feng S, Pawling J, Delgado-Sainz I, Ferguson MW, Dennis JW, Brown GW, Cortes-Ledesma F, Williams RS, Martin A, Xu D, Durocher D. Cell. 2020 Jul 23;182(2):481-496.e21. doi: 10.1016/j.cell.2020.05.040. Epub 2020 Jul 9. PubMed
SARS-CoV-2 Infection Depends on Cellular Heparan Sulfate and ACE2. Clausen TM, Sandoval DR, Spliid CB, Pihl J, Painter CD, Thacker BE, Glass CA, Narayanan A, Majowicz SA, Zhang Y, Torres JL, Golden GJ, Porell R, Garretson AF, Laubach L, Feldman J, Yin X, Pu Y, Hauser B, Caradonna TM, Kellman BP, Martino C, Gordts PLSM, Leibel SL, Chanda SK, Schmidt AG, Godula K, Jose J, Corbett KD, Ward AB, Carlin AF, Esko JD. bioRxiv. 2020 Jul 14. doi: 10.1101/2020.07.14.201616. PubMed
TRAIL-conjugated silver nanoparticles sensitize glioblastoma cells to TRAIL by regulating CHK1 in the DNA repair pathway. Sur-Erdem I, Muslu K, Pinarbasi N, Altunbek M, Seker-Polat F, Cingoz A, Aydin SO, Kahraman M, Culha M, Solaroglu I, Bagci-Onder T. Neurol Res. 2020 Dec;42(12):1061-1069. doi: 10.1080/01616412.2020.1796378. Epub 2020 Jul 25. PubMed
DNA methylation repels binding of hypoxia-inducible transcription factors to maintain tumor immunotolerance. D'Anna F, Van Dyck L, Xiong J, Zhao H, Berrens RV, Qian J, Bieniasz-Krzywiec P, Chandra V, Schoonjans L, Matthews J, De Smedt J, Minnoye L, Amorim R, Khorasanizadeh S, Yu Q, Zhao L, De Borre M, Savvides SN, Simon MC, Carmeliet P, Reik W, Rastinejad F, Mazzone M, Thienpont B, Lambrechts D. Genome Biol. 2020 Jul 27;21(1):182. doi: 10.1186/s13059-020-02087-z. PubMed
ABHD11 Is Critical for Embryonic Stem Cell Expansion, Differentiation and Lipid Metabolic Homeostasis. Liu G, Ruan Y, Zhang J, Wang X, Wu W, He P, Wang J, Xiong J, Cheng Y, Liu L, Yang Y, Tian Y, Jian R. Front Cell Dev Biol. 2020 Jul 7;8:570. doi: 10.3389/fcell.2020.00570. eCollection 2020. PubMed
Genetic evidence for an inhibitory role of tomosyn in insulin-stimulated GLUT4 exocytosis. Wang S, Liu Y, Crisman L, Wan C, Miller J, Yu H, Shen J. Traffic. 2020 Oct;21(10):636-646. doi: 10.1111/tra.12760. PubMed
Cell Cycle Checkpoints Cooperate to Suppress DNA- and RNA-Associated Molecular Pattern Recognition and Anti-Tumor Immune Responses. Chen J, Harding SM, Natesan R, Tian L, Benci JL, Li W, Minn AJ, Asangani IA, Greenberg RA. Cell Rep. 2020 Sep 1;32(9):108080. doi: 10.1016/j.celrep.2020.108080. PubMed
A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis. Zhang Q, Cao C, Gong W, Bao K, Wang Q, Wang Y, Bi L, Ma S, Zhao J, Liu L, Tian S, Zhang K, Yang J, Yao Z, Song N, Shi L. Theranostics. 2020 Aug 29;10(23):10769-10790. doi: 10.7150/thno.46878. eCollection 2020. PubMed
SARS-CoV-2 Infection Depends on Cellular Heparan Sulfate and ACE2. Clausen TM, Sandoval DR, Spliid CB, Pihl J, Perrett HR, Painter CD, Narayanan A, Majowicz SA, Kwong EM, McVicar RN, Thacker BE, Glass CA, Yang Z, Torres JL, Golden GJ, Bartels PL, Porell RN, Garretson AF, Laubach L, Feldman J, Yin X, Pu Y, Hauser BM, Caradonna TM, Kellman BP, Martino C, Gordts PLSM, Chanda SK, Schmidt AG, Godula K, Leibel SL, Jose J, Corbett KD, Ward AB, Carlin AF, Esko JD. Cell. 2020 Nov 12;183(4):1043-1057.e15. doi: 10.1016/j.cell.2020.09.033. Epub 2020 Sep 14. PubMed
A web tool for the design of prime-editing guide RNAs. Chow RD, Chen JS, Shen J, Chen S. Nat Biomed Eng. 2021 Feb;5(2):190-194. doi: 10.1038/s41551-020-00622-8. Epub 2020 Sep 28. PubMed
A saturating mutagenesis CRISPR-Cas9-mediated functional genomic screen identifies cis- and trans-regulatory elements of Oct4 in murine ESCs. Canver MC, Tripathi P, Bullen MJ, Olshansky M, Kumar Y, Wong LH, Turner SJ, Lessard S, Pinello L, Orkin SH, Das PP. J Biol Chem. 2020 Nov 20;295(47):15797-15809. doi: 10.1074/jbc.RA120.013772. Epub 2020 Sep 29. PubMed
Functional interrogation of HOXA9 regulome in MLLr leukemia via reporter-based CRISPR/Cas9 screen. Zhang H, Zhang Y, Zhou X, Wright S, Hyle J, Zhao L, An J, Zhao X, Shao Y, Xu B, Lee HM, Chen T, Zhou Y, Chen X, Lu R, Li C. Elife. 2020 Oct 1;9. pii: 57858. doi: 10.7554/eLife.57858. PubMed Associated Plasmids Lenti-human-TF-gRNA-puromycin
BRD4 modulates vulnerability of triple-negative breast cancer to targeting of integrin-dependent signaling pathways. Zhang Y, Xu B, Shi J, Li J, Lu X, Xu L, Yang H, Hamad N, Wang C, Napier D, He S, Liu C, Liu Z, Qian H, Chen L, Wei X, Zheng X, Huang JA, Thibault O, Craven R, Wei D, Pan Y, Zhou BP, Wu Y, Yang XH. Cell Oncol (Dordr). 2020 Dec;43(6):1049-1066. doi: 10.1007/s13402-020-00537-1. Epub 2020 Oct 2. PubMed
FBXO11 is a candidate tumor suppressor in the leukemic transformation of myelodysplastic syndrome. Schieber M, Marinaccio C, Bolanos LC, Haffey WD, Greis KD, Starczynowski DT, Crispino JD. Blood Cancer J. 2020 Oct 6;10(10):98. doi: 10.1038/s41408-020-00362-7. PubMed
Truncated HDAC9 identified by integrated genome-wide screen as the key modulator for paclitaxel resistance in triple-negative breast cancer. Lian B, Pei YC, Jiang YZ, Xue MZ, Li DQ, Li XG, Zheng YZ, Liu XY, Qiao F, Sun WL, Ling H, He M, Yao L, Hu X, Shao ZM. Theranostics. 2020 Sep 2;10(24):11092-11109. doi: 10.7150/thno.44997. eCollection 2020. PubMed
Endogenous retroviruses drive KRAB zinc-finger protein family expression for tumor suppression. Ito J, Kimura I, Soper A, Coudray A, Koyanagi Y, Nakaoka H, Inoue I, Turelli P, Trono D, Sato K. Sci Adv. 2020 Oct 21;6(43). pii: 6/43/eabc3020. doi: 10.1126/sciadv.abc3020. Print 2020 Oct. PubMed
Type I interferon signaling mediates Mycobacterium tuberculosis-induced macrophage death. Zhang L, Jiang X, Pfau D, Ling Y, Nathan CF. J Exp Med. 2021 Feb 1;218(2). pii: 211515. doi: 10.1084/jem.20200887. PubMed
Genome-wide CRISPR Screens Reveal Host Factors Critical for SARS-CoV-2 Infection. Wei J, Alfajaro MM, DeWeirdt PC, Hanna RE, Lu-Culligan WJ, Cai WL, Strine MS, Zhang SM, Graziano VR, Schmitz CO, Chen JS, Mankowski MC, Filler RB, Ravindra NG, Gasque V, de Miguel FJ, Patil A, Chen H, Oguntuyo KY, Abriola L, Surovtseva YV, Orchard RC, Lee B, Lindenbach BD, Politi K, van Dijk D, Kadoch C, Simon MD, Yan Q, Doench JG, Wilen CB. Cell. 2021 Jan 7;184(1):76-91.e13. doi: 10.1016/j.cell.2020.10.028. Epub 2020 Oct 20. PubMed Associated Plasmids Green monkey (Chlorocebus sabaeus) sgRNA library
G9a Promotes Breast Cancer Recurrence through Repression of a Pro-inflammatory Program. Mabe NW, Garcia NMG, Wolery SE, Newcomb R, Meingasner RC, Vilona BA, Lupo R, Lin CC, Chi JT, Alvarez JV. Cell Rep. 2020 Nov 3;33(5):108341. doi: 10.1016/j.celrep.2020.108341. PubMed
The hexosamine biosynthesis pathway is a targetable liability in KRAS/LKB1 mutant lung cancer. Kim J, Lee HM, Cai F, Ko B, Yang C, Lieu EL, Muhammad N, Rhyne S, Li K, Haloul M, Gu W, Faubert B, Kaushik AK, Cai L, Kasiri S, Marriam U, Nham K, Girard L, Wang H, Sun X, Kim J, Minna JD, Unsal-Kacmaz K, DeBerardinis RJ. Nat Metab. 2020 Dec;2(12):1401-1412. doi: 10.1038/s42255-020-00316-0. Epub 2020 Nov 30. PubMed
Functional characterization of uveal melanoma oncogenes. Ma J, Weng L, Bastian BC, Chen X. Oncogene. 2021 Jan;40(4):806-820. doi: 10.1038/s41388-020-01569-5. Epub 2020 Dec 1. PubMed
CRISPR screen identifies genes that sensitize AML cells to double-negative T-cell therapy. Soares F, Chen B, Lee JB, Ahmed M, Ly D, Tin E, Kang H, Zeng Y, Akhtar N, Minden MD, He HH, Zhang L. Blood. 2021 Apr 22;137(16):2171-2181. doi: 10.1182/blood.2019004108. PubMed
Muscle progenitor specification and myogenic differentiation are associated with changes in chromatin topology. Zhang N, Mendieta-Esteban J, Magli A, Lilja KC, Perlingeiro RCR, Marti-Renom MA, Tsirigos A, Dynlacht BD. Nat Commun. 2020 Dec 4;11(1):6222. doi: 10.1038/s41467-020-19999-w. PubMed
The long non-coding RNA LUCAT1 is a negative feedback regulator of interferon responses in humans. Agarwal S, Vierbuchen T, Ghosh S, Chan J, Jiang Z, Kandasamy RK, Ricci E, Fitzgerald KA. Nat Commun. 2020 Dec 11;11(1):6348. doi: 10.1038/s41467-020-20165-5. PubMed
TMEM41B Is a Pan-flavivirus Host Factor. Hoffmann HH, Schneider WM, Rozen-Gagnon K, Miles LA, Schuster F, Razooky B, Jacobson E, Wu X, Yi S, Rudin CM, MacDonald MR, McMullan LK, Poirier JT, Rice CM. Cell. 2021 Jan 7;184(1):133-148.e20. doi: 10.1016/j.cell.2020.12.005. Epub 2020 Dec 9. PubMed
A stress-induced tyrosine-tRNA depletion response mediates codon-based translational repression and growth suppression. Huh D, Passarelli MC, Gao J, Dusmatova SN, Goin C, Fish L, Pinzaru AM, Molina H, Ren Z, McMillan EA, Asgharian H, Goodarzi H, Tavazoie SF. EMBO J. 2021 Jan 15;40(2):e106696. doi: 10.15252/embj.2020106696. Epub 2020 Dec 21. PubMed
Manipulation of the human tRNA pool reveals distinct tRNA sets that act in cellular proliferation or cell cycle arrest. Aharon-Hefetz N, Frumkin I, Mayshar Y, Dahan O, Pilpel Y, Rak R. Elife. 2020 Dec 24;9. pii: 58461. doi: 10.7554/eLife.58461. PubMed
TMEM70 forms oligomeric scaffolds within mitochondrial cristae promoting in situ assembly of mammalian ATP synthase proton channel. Bahri H, Buratto J, Rojo M, Dompierre JP, Salin B, Blancard C, Cuvellier S, Rose M, Ben Ammar Elgaaied A, Tetaud E, di Rago JP, Devin A, Duvezin-Caubet S. Biochim Biophys Acta Mol Cell Res. 2021 Apr;1868(4):118942. doi: 10.1016/j.bbamcr.2020.118942. Epub 2020 Dec 23. PubMed
UFMylation inhibits the proinflammatory capacity of interferon-gamma-activated macrophages. Balce DR, Wang YT, McAllaster MR, Dunlap BF, Orvedahl A, Hykes BL Jr, Droit L, Handley SA, Wilen CB, Doench JG, Orchard RC, Stallings CL, Virgin HW. Proc Natl Acad Sci U S A. 2021 Jan 5;118(1). pii: 2011763118. doi: 10.1073/pnas.2011763118. PubMed
Genome-Scale Identification of SARS-CoV-2 and Pan-coronavirus Host Factor Networks. Schneider WM, Luna JM, Hoffmann HH, Sanchez-Rivera FJ, Leal AA, Ashbrook AW, Le Pen J, Ricardo-Lax I, Michailidis E, Peace A, Stenzel AF, Lowe SW, MacDonald MR, Rice CM, Poirier JT. Cell. 2021 Jan 7;184(1):120-132.e14. doi: 10.1016/j.cell.2020.12.006. Epub 2020 Dec 9. PubMed
Xpo7 negatively regulates Hedgehog signaling by exporting Gli2 from the nucleus. Markiewicz L, Uspienski T, Baran B, Niedziolka SM, Niewiadomski P. Cell Signal. 2021 Apr;80:109907. doi: 10.1016/j.cellsig.2020.109907. Epub 2020 Dec 28. PubMed
Functional Genomics Identify Distinct and Overlapping Genes Mediating Resistance to Different Classes of Heterobifunctional Degraders of Oncoproteins. Shirasaki R, Matthews GM, Gandolfi S, de Matos Simoes R, Buckley DL, Raja Vora J, Sievers QL, Bruggenthies JB, Dashevsky O, Poarch H, Tang H, Bariteau MA, Sheffer M, Hu Y, Downey-Kopyscinski SL, Hengeveld PJ, Glassner BJ, Dhimolea E, Ott CJ, Zhang T, Kwiatkowski NP, Laubach JP, Schlossman RL, Richardson PG, Culhane AC, Groen RWJ, Fischer ES, Vazquez F, Tsherniak A, Hahn WC, Levy J, Auclair D, Licht JD, Keats JJ, Boise LH, Ebert BL, Bradner JE, Gray NS, Mitsiades CS. Cell Rep. 2021 Jan 5;34(1):108532. doi: 10.1016/j.celrep.2020.108532. PubMed
Direct Intracellular Visualization of Ebola Virus-Receptor Interaction by In Situ Proximity Ligation. Mittler E, Alkutkar T, Jangra RK, Chandran K. mBio. 2021 Jan 12;12(1). pii: mBio.03100-20. doi: 10.1128/mBio.03100-20. PubMed
m(6)A RNA methylation regulates the fate of endogenous retroviruses. Chelmicki T, Roger E, Teissandier A, Dura M, Bonneville L, Rucli S, Dossin F, Fouassier C, Lameiras S, Bourc'his D. Nature. 2021 Mar;591(7849):312-316. doi: 10.1038/s41586-020-03135-1. Epub 2021 Jan 13. PubMed
Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying functional circRNAs. Zhang Y, Nguyen TM, Zhang XO, Wang L, Phan T, Clohessy JG, Pandolfi PP. Genome Biol. 2021 Jan 21;22(1):41. doi: 10.1186/s13059-021-02263-9. PubMed
G3BPs tether the TSC complex to lysosomes and suppress mTORC1 signaling. Prentzell MT, Rehbein U, Cadena Sandoval M, De Meulemeester AS, Baumeister R, Brohee L, Berdel B, Bockwoldt M, Carroll B, Chowdhury SR, von Deimling A, Demetriades C, Figlia G, de Araujo MEG, Heberle AM, Heiland I, Holzwarth B, Huber LA, Jaworski J, Kedra M, Kern K, Kopach A, Korolchuk VI, van 't Land-Kuper I, Macias M, Nellist M, Palm W, Pusch S, Ramos Pittol JM, Reil M, Reintjes A, Reuter F, Sampson JR, Scheldeman C, Siekierska A, Stefan E, Teleman AA, Thomas LE, Torres-Quesada O, Trump S, West HD, de Witte P, Woltering S, Yordanov TE, Zmorzynska J, Opitz CA, Thedieck K. Cell. 2021 Feb 4;184(3):655-674.e27. doi: 10.1016/j.cell.2020.12.024. Epub 2021 Jan 25. PubMed
CRISPRi enables isoform-specific loss-of-function screens and identification of gastric cancer-specific isoform dependencies. Davies R, Liu L, Taotao S, Tuano N, Chaturvedi R, Huang KK, Itman C, Mandoli A, Qamra A, Hu C, Powell D, Daly RJ, Tan P, Rosenbluh J. Genome Biol. 2021 Jan 26;22(1):47. doi: 10.1186/s13059-021-02266-6. PubMed
SARS-CoV-2 triggers an MDA-5-dependent interferon response which is unable to control replication in lung epithelial cells. Rebendenne A, Valadao ALC, Tauziet M, Maarifi G, Bonaventure B, McKellar J, Planes R, Nisole S, Arnaud-Arnould M, Moncorge O, Goujon C. J Virol. 2021 Jan 29. pii: JVI.02415-20. doi: 10.1128/JVI.02415-20. PubMed Associated Plasmids RRL.sin.cPPT.SFFV/Ace2.IRES-puro.WPRE (MT126) RRL.sin.cPPT.SFFV/Ace2.IRES-neo.WPRE (MT129) RRL.sin.cPPT.SFFV/Ace2.WPRE (MT136) RRL.sin.cPPT.SFFV/TMPRSS2 (variant 1).IRES-neo.WPRE (MT130)
LETR1 is a lymphatic endothelial-specific lncRNA governing cell proliferation and migration through KLF4 and SEMA3C. Ducoli L, Agrawal S, Sibler E, Kouno T, Tacconi C, Hon CC, Berger SD, Mullhaupt D, He Y, Kim J, D'Addio M, Dieterich LC, Carninci P, de Hoon MJL, Shin JW, Detmar M. Nat Commun. 2021 Feb 10;12(1):925. doi: 10.1038/s41467-021-21217-0. PubMed
Advanced single-cell pooled CRISPR screening identifies C19orf53 required for cell proliferation based on mTORC1 regulators. Tang Y, Liao S, Liu G, Xiong X, Liu H, Li F, Tan Z, Kong X, Yin Y, Tan B. Cell Biol Toxicol. 2021 Feb 13. pii: 10.1007/s10565-021-09586-0. doi: 10.1007/s10565-021-09586-0. PubMed
Modulating HSF1 levels impacts expression of the estrogen receptor alpha and antiestrogen response. Silveira MA, Tav C, Berube-Simard FA, Cuppens T, Leclercq M, Fournier E, Cote MC, Droit A, Bilodeau S. Life Sci Alliance. 2021 Feb 16;4(5). pii: 4/5/e202000811. doi: 10.26508/lsa.202000811. Print 2021 May. PubMed
Functional interrogation of DNA damage response variants with base editing screens. Cuella-Martin R, Hayward SB, Fan X, Chen X, Huang JW, Taglialatela A, Leuzzi G, Zhao J, Rabadan R, Lu C, Shen Y, Ciccia A. Cell. 2021 Feb 18;184(4):1081-1097.e19. doi: 10.1016/j.cell.2021.01.041. PubMed
Susceptibility-Associated Genetic Variation in NEDD9 Contributes to Prostate Cancer Initiation and Progression. Han D, Owiredu JN, Healy BM, Li M, Labaf M, Steinfeld JS, Patalano S, Gao S, Liu M, Macoska JA, Zarringhalam K, Siegfried KR, Yuan X, Rebbeck TR, Cai C. Cancer Res. 2021 Jul 15;81(14):3766-3776. doi: 10.1158/0008-5472.CAN-20-3042. Epub 2021 Feb 25. PubMed
In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8(+) T cell fate decisions. Huang H, Zhou P, Wei J, Long L, Shi H, Dhungana Y, Chapman NM, Fu G, Saravia J, Raynor JL, Liu S, Palacios G, Wang YD, Qian C, Yu J, Chi H. Cell. 2021 Mar 4;184(5):1245-1261.e21. doi: 10.1016/j.cell.2021.02.021. Epub 2021 Feb 25. PubMed
PlasmidHawk improves lab of origin prediction of engineered plasmids using sequence alignment. Wang Q, Kille B, Liu TR, Elworth RAL, Treangen TJ. Nat Commun. 2021 Feb 26;12(1):1167. doi: 10.1038/s41467-021-21180-w. PubMed
Transcription factor competition at the gamma-globin promoters controls hemoglobin switching. Liu N, Xu S, Yao Q, Zhu Q, Kai Y, Hsu JY, Sakon P, Pinello L, Yuan GC, Bauer DE, Orkin SH. Nat Genet. 2021 Apr;53(4):511-520. doi: 10.1038/s41588-021-00798-y. Epub 2021 Mar 1. PubMed
Engineered IgG1-Fc Molecules Define Valency Control of Cell Surface Fcgamma Receptor Inhibition and Activation in Endosomes. Bailey EM, Choudhury A, Vuppula H, Ortiz DF, Schaeck J, Manning AM, Bosques CJ, Hoppe AD. Front Immunol. 2021 Feb 15;11:617767. doi: 10.3389/fimmu.2020.617767. eCollection 2020. PubMed
Identification of pathogenic variants in cancer genes using base editing screens with editing efficiency correction. Huang C, Li G, Wu J, Liang J, Wang X. Genome Biol. 2021 Mar 10;22(1):80. doi: 10.1186/s13059-021-02305-2. PubMed
SARS-CoV-2 hijacks folate and one-carbon metabolism for viral replication. Zhang Y, Guo R, Kim SH, Shah H, Zhang S, Liang JH, Fang Y, Gentili M, Leary CNO, Elledge SJ, Hung DT, Mootha VK, Gewurz BE. Nat Commun. 2021 Mar 15;12(1):1676. doi: 10.1038/s41467-021-21903-z. PubMed
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RNA-binding protein ELAVL4/HuD ameliorates Alzheimer's disease-related molecular changes in human iPSC-derived neurons. van der Linden RJ, Gerritsen JS, Liao M, Widomska J, Pearse RV 2nd, White FM, Franke B, Young-Pearse TL, Poelmans G. Prog Neurobiol. 2022 Oct;217:102316. doi: 10.1016/j.pneurobio.2022.102316. Epub 2022 Jul 14. PubMed
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Cytoplasmic keratins couple with and maintain nuclear envelope integrity in colonic epithelial cells. Stenvall CA, Nystrom JH, Butler-Hallissey C, Jansson T, Heikkila TRH, Adam SA, Foisner R, Goldman RD, Ridge KM, Toivola DM. Mol Biol Cell. 2022 Nov 1;33(13):ar121. doi: 10.1091/mbc.E20-06-0387. Epub 2022 Aug 24. PubMed
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ABL kinases regulate translation in HER2+ cells through Y-box-binding protein 1 to facilitate colonization of the brain. McKernan CM, Khatri A, Hannigan M, Child J, Chen Q, Mayro B, Snyder D, Nicchitta CV, Pendergast AM. Cell Rep. 2022 Aug 30;40(9):111268. doi: 10.1016/j.celrep.2022.111268. PubMed
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A translational genomics approach identifies IL10RB as the top candidate gene target for COVID-19 susceptibility. Voloudakis G, Vicari JM, Venkatesh S, Hoffman GE, Dobrindt K, Zhang W, Beckmann ND, Higgins CA, Argyriou S, Jiang S, Hoagland D, Gao L, Corvelo A, Cho K, Lee KM, Bian J, Lee JS, Iyengar SK, Luoh SW, Akbarian S, Striker R, Assimes TL, Schadt EE, Lynch JA, Merad M, tenOever BR, Charney AW, Brennand KJ, Fullard JF, Roussos P. NPJ Genom Med. 2022 Sep 5;7(1):52. doi: 10.1038/s41525-022-00324-x. PubMed
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