Genome-scale pan-cancer interrogation of lncRNA dependencies using CasRx.
Montero JJ, Trozzo R, Sugden M, Ollinger R, Belka A, Zhigalova E, Waetzig P, Engleitner T, Schmidt-Supprian M, Saur D, Rad R
Nat Methods. 2024 Feb 26. doi: 10.1038/s41592-024-02190-0. PubMed Article
Montero JJ, Trozzo R, Sugden M, Ollinger R, Belka A, Zhigalova E, Waetzig P, Engleitner T, Schmidt-Supprian M, Saur D, Rad R
Nat Methods. 2024 Feb 26. doi: 10.1038/s41592-024-02190-0. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 212961 | pLKO5.U6.DR130(CasRX)_Empty.EFS.tRFP657 | Lentiviral plasmid for the cloning of mature gRNAs of the RfxCas13d (CasRx) CRISPR system. As a marker, the plasmid also encodes the tRFP657 fluorescence protein. |
| 212962 | pLKO5.U6.DR130(CasRX)_Non-targeting sgRNA.EFS.tRFP657 | Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein. |
| 212963 | pLKO5.U6.DR130(CasRX)_GFP-targeting sgRNA.EFS.tRFP657 | Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein. |
| 212965 | pTransposon.CAG.CasRX-P2A-Blast | Episomal plasmid encoding CasRx-P2A under the control of the CAG promoter. The construct is flanked by PB and SB repeats for integration into the cell genome. |
| 212966 | Pan-cancer CasRx lncRNA Albarossa library | The human CasRx CRISPR lentiviral library encodes 75,725 gRNA arrays (two gRNAs per array) targeting 24,171 lncRNAs. |