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PurposeEpisomal plasmid encoding CasRx-P2A under the control of the CAG promoter. The construct is flanked by PB and SB repeats for integration into the cell genome.
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Depositing Lab
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Depositing OrganizationKlinikum der Technischen Universität München (TUM Klinikum)
Located in Germany -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 212965 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 212965-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 212965-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepA3
- Total vector size (bp) 10329
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Vector typeMammalian Expression ; transposon
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCasRx
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Alt nameCas13d Ruminococcus flavefaciens XPD3002
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SpeciesRuminococcus flavefaciens XPD3002
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Insert Size (bp)3456
- Promoter CAGGS
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Tag
/ Fusion Protein
- P2A-Blasticidin resistance (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 212965-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 212965-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTransposon.CAG.CasRX-P2A-Blast was a gift from Roland Rad (Addgene plasmid # 212965 ; http://n2t.net/addgene:212965 ; RRID:Addgene_212965) -
For your References section:
Genome-scale pan-cancer interrogation of lncRNA dependencies using CasRx. Montero JJ, Trozzo R, Sugden M, Ollinger R, Belka A, Zhigalova E, Waetzig P, Engleitner T, Schmidt-Supprian M, Saur D, Rad R. Nat Methods. 2024 Feb 26. doi: 10.1038/s41592-024-02190-0. 10.1038/s41592-024-02190-0 PubMed 38409225
