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Image-based, pooled phenotyping reveals multidimensional, disease-specific variant effects.

Pendyala S, Partington K, Bradley N, McEwen AE, Straub G, Kim HJ, Fayer S, Holmes DL, Sitko KA, Vandi AJ, Powell RL, Friedman CE, McDermot E, Kishore N, Roth FP, Rubin AF, Yang KC, Starita LM, Noble WS, Fowler DM
bioRxiv [Preprint]. 2025 Jul 5:2025.07.03.663081. doi: 10.1101/2025.07.03.663081. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
252243PB-circBC-UCOE-EF1a-mEGFP(NTag)-IRES-PuroR-WPREVIS-seq expression plasmid
252244PB-circBC-UCOE-EF1a-mEGFP(CTag)-IRES-PuroR-WPREVIS-seq expression plasmid
252245PB-circBC-UCOE-CAG-mEGFP(NTag)-IRES-PuroR-WPREVIS-seq expression plasmid

Antibodies from Article