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PB-circBC-UCOE-EF1a-mEGFP(CTag)-IRES-PuroR-WPRE
(Plasmid #252244)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252244 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    p051_PB_tornado
  • Backbone manufacturer
    Jay Shendure, JB Lalanne, Sam Regalado
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin
  • Tag / Fusion Protein
    • mEGFP (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • mEGFP (C terminal on backbone)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A FISSEQ capable barcode must first be cloned into this plasmid via BsmBI/Esp3I and a gene or ORF library must be cloned in via SapI before transfection. This vector must be co-transfected with another vector containing the piggybac transposase in order to integrate into the genome.

Please visit https://www.biorxiv.org/content/10.1101/2025.07.03.663081v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-circBC-UCOE-EF1a-mEGFP(CTag)-IRES-PuroR-WPRE was a gift from Douglas Fowler (Addgene plasmid # 252244 ; http://n2t.net/addgene:252244 ; RRID:Addgene_252244)

  • For your References section:

    Image-based, pooled phenotyping reveals multidimensional, disease-specific variant effects. Pendyala S, Partington K, Bradley N, McEwen AE, Straub G, Kim HJ, Fayer S, Holmes DL, Sitko KA, Vandi AJ, Powell RL, Friedman CE, McDermot E, Kishore N, Roth FP, Rubin AF, Yang KC, Starita LM, Noble WS, Fowler DM. bioRxiv [Preprint]. 2025 Jul 5:2025.07.03.663081. doi: 10.1101/2025.07.03.663081. 10.1101/2025.07.03.663081 PubMed 40631108
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