pcDNA4 HisMax-V5-GFP-RRBP1
(Plasmid #92150)

• Purpose: Expresses V5- and EGFP-tagged human RRBP1 (with 54 decapeptide repeats) in mammalian cells.
• Depositing Lab: Alice Ting
• Publication: Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi: 10.7554/eLife.24463.

Backbone

• Vector backbone: pcDNA4 HisMax
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5300
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: RRBP1
• Alt name: p180
• Species: H. sapiens (human)
• Insert Size (bp): 4620
• Mutation: In RRBP1: N951S and T1329L. E1279 is also missing.
• Entrez Gene: RRBP1 (a.k.a. ES/130, ES130, RRp, hES, p180)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Calreticulin signal peptide (N terminal on insert)
  • V5 (N terminal on insert)
  • EGFP (A207K mutation) (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMVf
• 3′ sequencing primer: BGHr

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: pcDNA4 HisMax-GFP-RRBP1 was obtained from Alexander F. Palazzo (University of Toronto).
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry