Skip to main content
Addgene
Open Menu

α-satellite pSP73
(Plasmid #263227)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 263227 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart

Backbone

  • Vector backbone
    pSP73
  • Backbone size w/o insert (bp) 2456
  • Total vector size (bp) 2772
  • Modifications to backbone
    alpha-satellite repeats were amplified by PCR on gDNA extracted from HT1080 cell line and cloned in the EcoRV site of the backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    alpha-satellite repeats
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    316
  • GenBank ID
    M81230.1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The insert has a dimeric α-satellite unit (two monomers, 171 bp and ~168 bp, 82% identical, with an intact CENP-B box in the second monomer) cloned at the EcoRV site downstream of the SP6 promoter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    α-satellite pSP73 was a gift from Ylli Doksani (Addgene plasmid # 263227 ; http://n2t.net/addgene:263227 ; RRID:Addgene_263227)

  • For your References section:

    Damage-induced i-loops generate eccDNA from repetitive elements. Zanella E, Giannattasio M, Bisi S, Nicassio F, Doksani Y. Mol Cell. 2026 May 21;86(10):1856-1869.e11. doi: 10.1016/j.molcel.2026.04.003. Epub 2026 Apr 30. 10.1016/j.molcel.2026.04.003 PubMed 42066753
Related items: