Damage-induced i-loops generate eccDNA from repetitive elements.
Zanella E, Giannattasio M, Bisi S, Nicassio F, Doksani Y
Mol Cell. 2026 May 21;86(10):1856-1869.e11. doi: 10.1016/j.molcel.2026.04.003. Epub 2026 Apr 30.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 263221 | Lenti-iCas9(D10A)-Neo | Lentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9(D10A), separated by a P2A self-cleavage sequence |
| 263222 | Lenti-iCas9(H840A)-Neo | Lentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9(H840A), separated by a P2A self-cleavage sequence |
| 263223 | Teloguide-Puro | lentiGuide-Puro backbone with cloned target sequence for mammalian telomere. The insert is: 5´- GTTAGGGTTAGGGTTAGGGTTA-3´. |
| 263224 | Alphaguide-Puro | lentiGuide-Puro backbone with cloned target sequence for human alpha-satellite repeats. The insert is: 5´-GAATCTGCAAGTGGATATTG-3´. |
| 263227 | α-satellite pSP73 | pSP73 backbone carrying 316 bp of alpha-satellite DNA obtained from HT1080 cell line by PCR |