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Lenti-iCas9(D10A)-Neo
(Plasmid #263221)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 263221 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    Lenti-iCas9-neo (Addgene #85400)
  • Modifications to backbone
    D10A point mutation in spCas9 sequence
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Neomycin (select with G418) ; EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Flag-iCas9(D10A)-P2A-GFP
  • Species
    Synthetic
  • Insert Size (bp)
    5019
  • Mutation
    D10A
  • Tag / Fusion Protein
    • FLAG

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Qin Yan Lab (Addgene Plasmid #85400)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lenti-iCas9(D10A)-Neo was a gift from Ylli Doksani (Addgene plasmid # 263221 ; http://n2t.net/addgene:263221 ; RRID:Addgene_263221)

  • For your References section:

    Damage-induced i-loops generate eccDNA from repetitive elements. Zanella E, Giannattasio M, Bisi S, Nicassio F, Doksani Y. Mol Cell. 2026 May 21;86(10):1856-1869.e11. doi: 10.1016/j.molcel.2026.04.003. Epub 2026 Apr 30. 10.1016/j.molcel.2026.04.003 PubMed 42066753
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